{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T21:13:00Z","timestamp":1768511580801,"version":"3.49.0"},"posted":{"date-parts":[[2024,3,30]]},"group-title":"Genetics","reference-count":20,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,3,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  CRISPR\/Cas9 methods are a powerful\n                  <jats:italic>in vivo<\/jats:italic>\n                  approach to edit the genome of\n                  <jats:italic>Drosophila melanogaster<\/jats:italic>\n                  . To convert existing\n                  <jats:italic>Drosophila GAL4<\/jats:italic>\n                  lines to\n                  <jats:italic>LexA<\/jats:italic>\n                  driver lines in a secondary school classroom setting, we applied the CRISPR-based genetic approach to a collection of\n                  <jats:italic>Gal4<\/jats:italic>\n                  \u2018driver\u2019 lines. The integration of the\n                  <jats:italic>yellow<\/jats:italic>\n                  <jats:sup>+<\/jats:sup>\n                  coat color marker into homology-assisted CRISPR knock-in (HACK) enabled visual selection of\n                  <jats:italic>Gal4-to-LexA<\/jats:italic>\n                  conversions using brightfield stereo-microscopy available in a broader set of standard classrooms. Here, we report the successful conversion of eleven\n                  <jats:italic>Gal4<\/jats:italic>\n                  lines with expression in neuropeptide-expressing cells into corresponding, novel\n                  <jats:italic>LexA<\/jats:italic>\n                  drivers. The conversion was confirmed by\n                  <jats:italic>LexA-<\/jats:italic>\n                  and\n                  <jats:italic>Gal4-<\/jats:italic>\n                  specific GFP reporter gene expression. This curriculum was successfully implemented in a summer course running 16 hours\/week for seven weeks. The modularity, flexibility, and compactness of this course should enable development of similar classes in secondary schools and undergraduate curricula, to provide opportunities for experience-based science instruction, and university-secondary school collaborations that simultaneously fulfill research needs in the community of science.\n                <\/jats:p>","DOI":"10.1101\/2024.03.28.587232","type":"posted-content","created":{"date-parts":[[2024,3,30]],"date-time":"2024-03-30T23:55:20Z","timestamp":1711842920000},"source":"Crossref","is-referenced-by-count":0,"title":["CRISPR\/Cas9 gene editing in\n                  <i>Drosophila<\/i>\n                  via visual selection in a summer classroom"],"prefix":"10.64898","author":[{"given":"Lutz","family":"Kockel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jenna","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clara","family":"Gulick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madeleine 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