{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T21:00:31Z","timestamp":1768510831200,"version":"3.49.0"},"posted":{"date-parts":[[2024,4,5]]},"group-title":"Immunology","reference-count":44,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,4,5]],"date-time":"2024-04-05T00:00:00Z","timestamp":1712275200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"NIH","award":["R01 AI151167"],"award-info":[{"award-number":["R01 AI151167"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,9,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Lung-resident B cells are increasingly recognized as key contributors to protective immunity against respiratory viruses, yet the mechanisms that govern their generation and specialization remain poorly understood. Here, we identify B cell-intrinsic \u03b1v integrin as a critical negative regulator of germinal center (GC) dynamics and memory B cell formation in the lung following influenza A virus (IAV) infection. Using B cell-specific \u03b1v knockout mice, we show that loss of B cell \u03b1v integrin leads to persistent GC activity within the inducible bronchus-associated lymphoid tissue and expansion of lung-resident memory B cells, including IgA\n                  <jats:sup>+<\/jats:sup>\n                  and cross-reactive B cells capable of recognizing heterologous influenza variants. Single-cell transcriptomic and B cell receptor (BCR) sequence analyses reveal that \u03b1v restricts clonal expansion and antigenic diversification of GC and memory B cells in the lung, but not in draining lymph nodes, indicating a spatially restricted mechanism of mucosal B cell regulation. These findings position \u03b1v integrin as a key checkpoint that constrains local mucosal B cell evolution and suggest new strategies to improve mucosal vaccine efficacy by enhancing GC activity directly in the lung.\n                <\/jats:p>","DOI":"10.1101\/2024.04.03.587969","type":"posted-content","created":{"date-parts":[[2024,4,5]],"date-time":"2024-04-05T20:45:17Z","timestamp":1712349917000},"source":"Crossref","is-referenced-by-count":0,"title":["B cell \u03b1v integrin regulates tissue specialization and clonal expansion of lung germinal center and memory B cells after viral infection"],"prefix":"10.64898","author":[{"given":"Andrea","family":"Montiel-Armendariz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelsey","family":"Roe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan","family":"Lagos-Orellana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura Veronica","family":"Martinez-Castro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew J","family":"Dufort","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver","family":"Harrison","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam","family":"Lacy-Hulbert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mridu","family":"Acharya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025090915450884000_2024.04.03.587969v3.1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1801208"},{"key":"2025090915450884000_2024.04.03.587969v3.2","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-018-0260-6"},{"key":"2025090915450884000_2024.04.03.587969v3.3","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1115369109"},{"key":"2025090915450884000_2024.04.03.587969v3.4","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.abj5129"},{"key":"2025090915450884000_2024.04.03.587969v3.5","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2009.126"},{"key":"2025090915450884000_2024.04.03.587969v3.6","doi-asserted-by":"publisher","DOI":"10.1172\/JCI43796"},{"key":"2025090915450884000_2024.04.03.587969v3.7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1212597109"},{"key":"2025090915450884000_2024.04.03.587969v3.8","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0707421104"},{"key":"2025090915450884000_2024.04.03.587969v3.9","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.5.2610"},{"key":"2025090915450884000_2024.04.03.587969v3.10","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2682"},{"key":"2025090915450884000_2024.04.03.587969v3.11","doi-asserted-by":"publisher","DOI":"10.1038\/343170a0"},{"key":"2025090915450884000_2024.04.03.587969v3.12","doi-asserted-by":"crossref","unstructured":"Schrock, D.C. , et al., Pivotal role for alphaV integrins in sustained Tfh support of the germinal center response for long-lived plasma cell generation. 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