{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T06:00:47Z","timestamp":1782280847328,"version":"3.54.5"},"posted":{"date-parts":[[2024,4,9]]},"group-title":"Biochemistry","reference-count":25,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,4,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Polyamines are polycationic molecules that are crucial in a wide array of cellular functions. Their biosynthesis is mediated by aminopropyl transferases (APTs), promising targets in antimicrobial, antineoplastic and antineurodegenerative therapies. A major limitation, however, is the lack of high-throughput assays to measure their activity. We developed the first fluorescence-based assay, DAB-APT, for measurement of APT activity using 1,2-diacetyl benzene, which forms fluorescent conjugates with putrescine, spermidine and spermine with fluorescence intensity increasing with increasing carbon chain length. The assay has been validated using APT enzymes from\n                  <jats:italic>S. cerevisiae<\/jats:italic>\n                  and\n                  <jats:italic>P. falciparum<\/jats:italic>\n                  and is suitable for high-throughput screening of large chemical libraries. Given the importance of APTs in infectious diseases, cancer and neurobiology, our DAB-APT assay has broad applications, holding promise for advancing research and drug discovery efforts.\n                <\/jats:p>","DOI":"10.1101\/2024.04.09.588734","type":"posted-content","created":{"date-parts":[[2024,4,10]],"date-time":"2024-04-10T01:40:23Z","timestamp":1712713223000},"source":"Crossref","is-referenced-by-count":0,"title":["DAB-APT: a Fluorescence-Based Assay for Determining Aminopropyl Transferase Activity and Inhibition"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2318-5960","authenticated-orcid":false,"given":"Pallavi","family":"Singh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae-Yeon","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5028-1400","authenticated-orcid":false,"given":"Choukri Ben","family":"Mamoun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2024041109300737000_2024.04.09.588734v1.1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2018.00010"},{"key":"2024041109300737000_2024.04.09.588734v1.2","unstructured":"Cohen, S. 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