{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"medRxiv"}],"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T02:02:56Z","timestamp":1783735376635,"version":"3.55.0"},"posted":{"date-parts":[[2024,5,1]]},"group-title":"Infectious Diseases (except HIV\/AIDS)","reference-count":84,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Cross-reactive antibodies (Abs) to epitopes that span envelope proteins on the virion surface are hypothesized to protect against dengue. Here, we measured Abs targeting the quaternary envelope dimer epitope (EDE) as well as neutralizing and binding Abs and evaluate their association with dengue virus (DENV) infection, vaccine response, and disease outcome in dengue vaccinated and unvaccinated children (n=252) within a longitudinal cohort in Cebu, Philippines (n=2,996). Abs targeting EDE were prevalent and strongly associated with broad neutralization of DENV1-4 in those with baseline multitypic immunity. Subsequent natural infection and vaccination boosted EDE-like, neutralizing, and binding Abs. EDE-like Abs were associated with reduced dengue risk and mediated the protective effect of binding and neutralizing Abs on symptomatic and severe dengue. Thus, Abs targeting quaternary epitopes help explain broad cross protection in those with multiple prior DENV exposures, making them useful for evaluation and development of future vaccines and therapeutics.<\/jats:p>","DOI":"10.1101\/2024.04.30.24306574","type":"posted-content","created":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T02:15:20Z","timestamp":1714616120000},"source":"Crossref","is-referenced-by-count":2,"title":["Envelope-dimer epitope-like broadly protective antibodies against dengue in children following natural infection and vaccination"],"prefix":"10.64898","author":[{"given":"Patrick I.","family":"Mpingabo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Ylade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosemary A.","family":"Aogo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Vinna","family":"Crisostomo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devina J.","family":"Thiono","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jedas Veronica","family":"Daag","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristal-An","family":"Agrupis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Coello","family":"Escoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo L.","family":"Raimundi-Rodriguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila D.","family":"Odio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Abad","family":"Fernandez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"White","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aravinda M.","family":"de Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline","family":"Deen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1033-6758","authenticated-orcid":false,"given":"Leah C.","family":"Katzelnick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2024050606150498000_2024.04.30.24306574v1.1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001760"},{"key":"2024050606150498000_2024.04.30.24306574v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2019.12.002"},{"key":"2024050606150498000_2024.04.30.24306574v1.3","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0476-8"},{"key":"2024050606150498000_2024.04.30.24306574v1.4","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aax4144"},{"key":"2024050606150498000_2024.04.30.24306574v1.5","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1800820"},{"key":"2024050606150498000_2024.04.30.24306574v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2020.06.079"},{"key":"2024050606150498000_2024.04.30.24306574v1.7","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciac418"},{"key":"2024050606150498000_2024.04.30.24306574v1.8","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciac808"},{"key":"2024050606150498000_2024.04.30.24306574v1.9","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciab864"},{"key":"2024050606150498000_2024.04.30.24306574v1.10","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiac424"},{"key":"2024050606150498000_2024.04.30.24306574v1.11","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0009258"},{"key":"2024050606150498000_2024.04.30.24306574v1.12","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-023-00658-2"},{"key":"2024050606150498000_2024.04.30.24306574v1.13","doi-asserted-by":"publisher","DOI":"10.1016\/s1473-3099(20)30767-2"},{"key":"2024050606150498000_2024.04.30.24306574v1.14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002451"},{"key":"2024050606150498000_2024.04.30.24306574v1.15","doi-asserted-by":"publisher","DOI":"10.1186\/s41182-016-0004-y"},{"key":"2024050606150498000_2024.04.30.24306574v1.16","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1522136113"},{"key":"2024050606150498000_2024.04.30.24306574v1.17","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005554"},{"key":"2024050606150498000_2024.04.30.24306574v1.18","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201745302"},{"key":"2024050606150498000_2024.04.30.24306574v1.19","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abg4084"},{"key":"2024050606150498000_2024.04.30.24306574v1.20","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2463"},{"key":"2024050606150498000_2024.04.30.24306574v1.21","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1812055115"},{"key":"2024050606150498000_2024.04.30.24306574v1.22","doi-asserted-by":"publisher","DOI":"10.1126\/science.1185181"},{"key":"2024050606150498000_2024.04.30.24306574v1.23","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-031210-101315"},{"key":"2024050606150498000_2024.04.30.24306574v1.24","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.55"},{"key":"2024050606150498000_2024.04.30.24306574v1.25","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00871-13"},{"key":"2024050606150498000_2024.04.30.24306574v1.26","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00316-08"},{"key":"2024050606150498000_2024.04.30.24306574v1.27","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02273-12"},{"key":"2024050606150498000_2024.04.30.24306574v1.28","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00873-13"},{"key":"2024050606150498000_2024.04.30.24306574v1.29","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/152.9.793"},{"key":"2024050606150498000_2024.04.30.24306574v1.30","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001188"},{"key":"2024050606150498000_2024.04.30.24306574v1.31","doi-asserted-by":"publisher","DOI":"10.1126\/science.aan6836"},{"key":"2024050606150498000_2024.04.30.24306574v1.32","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3515"},{"key":"2024050606150498000_2024.04.30.24306574v1.33","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.AID-0022-2014"},{"key":"2024050606150498000_2024.04.30.24306574v1.34","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01205-17"},{"key":"2024050606150498000_2024.04.30.24306574v1.35","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2020.04.007"},{"key":"2024050606150498000_2024.04.30.24306574v1.36","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2017.07.045"},{"key":"2024050606150498000_2024.04.30.24306574v1.37","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003230"},{"key":"2024050606150498000_2024.04.30.24306574v1.38","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix609"},{"key":"2024050606150498000_2024.04.30.24306574v1.39","doi-asserted-by":"crossref","first-page":"297","DOI":"10.3390\/vaccines8020297","article-title":"A Simple and High-Throughput ELISA-Based Neutralization Assay for the Determination of Anti-Flavivirus Neutralizing Antibodies","volume":"8","year":"2020","journal-title":"Vaccines"},{"key":"2024050606150498000_2024.04.30.24306574v1.40","doi-asserted-by":"crossref","first-page":"e0005554","DOI":"10.1371\/journal.pntd.0005554","article-title":"Dissecting the human serum antibody response to secondary dengue virus infections","volume":"11","year":"2017","journal-title":"PLoS neglected tropical diseases"},{"key":"2024050606150498000_2024.04.30.24306574v1.41","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00273-15"},{"key":"2024050606150498000_2024.04.30.24306574v1.42","doi-asserted-by":"publisher","DOI":"10.1038\/nature14130"},{"key":"2024050606150498000_2024.04.30.24306574v1.43","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15411"},{"key":"2024050606150498000_2024.04.30.24306574v1.44","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3058"},{"key":"2024050606150498000_2024.04.30.24306574v1.45","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.52384"},{"key":"2024050606150498000_2024.04.30.24306574v1.46","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-016-0003-3"},{"key":"2024050606150498000_2024.04.30.24306574v1.47","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.11.010"},{"key":"2024050606150498000_2024.04.30.24306574v1.48","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006934"},{"key":"2024050606150498000_2024.04.30.24306574v1.49","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.00556-18"},{"key":"2024050606150498000_2024.04.30.24306574v1.50","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3849"},{"key":"2024050606150498000_2024.04.30.24306574v1.51","doi-asserted-by":"publisher","DOI":"10.1038\/nature18938"},{"key":"2024050606150498000_2024.04.30.24306574v1.52","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01461-15"},{"key":"2024050606150498000_2024.04.30.24306574v1.53","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(20)30392-2"},{"key":"2024050606150498000_2024.04.30.24306574v1.54","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2021.07.042"},{"key":"2024050606150498000_2024.04.30.24306574v1.55","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007280"},{"key":"2024050606150498000_2024.04.30.24306574v1.56","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.20-0937"},{"key":"2024050606150498000_2024.04.30.24306574v1.57","doi-asserted-by":"publisher","DOI":"10.2139\/ssrm.4411430"},{"key":"2024050606150498000_2024.04.30.24306574v1.58","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1200566109"},{"key":"2024050606150498000_2024.04.30.24306574v1.59","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001568"},{"key":"2024050606150498000_2024.04.30.24306574v1.60","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.6.2793-2797.2005"},{"key":"2024050606150498000_2024.04.30.24306574v1.61","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00918-23"},{"key":"2024050606150498000_2024.04.30.24306574v1.62","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2019.1643676"},{"key":"2024050606150498000_2024.04.30.24306574v1.63","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-021-01392-9"},{"key":"2024050606150498000_2024.04.30.24306574v1.64","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa304"},{"key":"2024050606150498000_2024.04.30.24306574v1.65","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.adi1734"},{"key":"2024050606150498000_2024.04.30.24306574v1.66","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2020.07.056"},{"key":"2024050606150498000_2024.04.30.24306574v1.67","doi-asserted-by":"publisher","DOI":"10.1172\/JCI147066"},{"key":"2024050606150498000_2024.04.30.24306574v1.68","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiw576"},{"key":"2024050606150498000_2024.04.30.24306574v1.69","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaw9066"},{"key":"2024050606150498000_2024.04.30.24306574v1.70","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-44330-8"},{"key":"2024050606150498000_2024.04.30.24306574v1.71","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abm3151"},{"key":"2024050606150498000_2024.04.30.24306574v1.72","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(24)00099-9"},{"key":"2024050606150498000_2024.04.30.24306574v1.73","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002952"},{"key":"2024050606150498000_2024.04.30.24306574v1.74","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2009.08-0625"},{"key":"2024050606150498000_2024.04.30.24306574v1.75","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0157-4"},{"key":"2024050606150498000_2024.04.30.24306574v1.76","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.19-0270"},{"key":"2024050606150498000_2024.04.30.24306574v1.77","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1704984114"},{"key":"2024050606150498000_2024.04.30.24306574v1.78","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02125"},{"key":"2024050606150498000_2024.04.30.24306574v1.79","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2016.06.022"},{"key":"2024050606150498000_2024.04.30.24306574v1.80","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.00386-22"},{"key":"2024050606150498000_2024.04.30.24306574v1.81","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006862"},{"key":"2024050606150498000_2024.04.30.24306574v1.82","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.10895"},{"key":"2024050606150498000_2024.04.30.24306574v1.83","doi-asserted-by":"publisher","DOI":"10.1037\/0022-3514.51.6.1173"},{"key":"2024050606150498000_2024.04.30.24306574v1.84","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiz618"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2024.04.30.24306574","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T19:59:44Z","timestamp":1768507184000},"score":1,"resource":{"primary":{"URL":"http:\/\/medrxiv.org\/lookup\/doi\/10.1101\/2024.04.30.24306574"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,1]]},"references-count":84,"URL":"https:\/\/doi.org\/10.1101\/2024.04.30.24306574","relation":{},"subject":[],"published":{"date-parts":[[2024,5,1]]},"subtype":"preprint"}}