{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T17:58:23Z","timestamp":1780423103502,"version":"3.54.1"},"posted":{"date-parts":[[2024,4,30]]},"group-title":"Microbiology","reference-count":50,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,4,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Reducing malaria transmission has been a major pillar of control programmes and is considered crucial for achieving malaria elimination. Gametocytes, the transmissible forms of the\n                  <jats:italic>P. falciparum<\/jats:italic>\n                  parasite, arise during the blood stage of the parasite and develop through 5 morphologically distinct stages. Immature gametocytes (stage I-IV) sequester and develop in the extravascular niche of the bone marrow and possibly spleen. Only mature stage V gametocytes re-enter peripheral circulation to be taken up by mosquitoes for successful onward transmission. We have recently shown that immature, but not mature gametocytes are targets of host immune responses and identified putative target surface antigens. We hypothesize that these antigens play a role in gametocyte sequestration and contribute to acquired transmission-reducing immunity. Here we demonstrate that surface antigen expression, serum reactivity by human IgG, and opsonic phagocytosis by macrophages all show similar dynamics during gametocyte maturation, i.e., on in immature and off in mature gametocytes. Moreover, the switch in surface reactivity coincides with reversal in phosphatidylserine (PS) surface exposure, a marker for red blood cell age and clearance. PS is exposed on the surface of immature gametocytes (as well as in late asexual stages) but is removed from the surface in later gametocyte stages (IV-V). Using parasite reverse genetics and drug perturbations, we confirm that parasite protein export into the host cell and phospholipid scramblase activity are required for the observed surface modifications in asexual and sexual\n                  <jats:italic>P. falciparum<\/jats:italic>\n                  stages. These findings suggest that the dynamic surface remodelling allows (i) immature gametocyte sequestration in bone marrow and (ii) mature gametocyte release into peripheral circulation and immune evasion, therefore contributing to mature gametocyte survival\n                  <jats:italic>in vivo<\/jats:italic>\n                  and onward transmission to mosquitoes. Importantly, blocking scramblase activity during gametocyte maturation results in efficient clearance of mature gametocytes, revealing a potential path for transmission blocking interventions. Our studies have important implications for our understanding of parasite biology and form a starting point for novel intervention strategies to simultaneously reduce parasite burden and transmission.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>IMPORTANT<\/jats:title>\n                  <jats:list list-type=\"bullet\">\n                    <jats:list-item>\n                      <jats:p>Manuscripts submitted to Review Commons are peer reviewed in a journal-agnostic way.<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>Upon transfer of the peer reviewed preprint to a journal, the referee reports will be available in full to the handling editor.<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>The identity of the referees will NOT be communicated to the authors unless the reviewers choose to sign their report.<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>The identity of the referee will be confidentially disclosed to any affiliate journals to which the manuscript is transferred.<\/jats:p>\n                    <\/jats:list-item>\n                  <\/jats:list>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>GUIDELINES<\/jats:title>\n                  <jats:list list-type=\"bullet\">\n                    <jats:list-item>\n                      <jats:p>\n                        For reviewers:\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/www.reviewcommons.org\/reviewers\">https:\/\/www.reviewcommons.org\/reviewers<\/jats:ext-link>\n                      <\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>\n                        For authors:\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/www.reviewcommons.org\/authors\">https:\/\/www.reviewcommons.org\/authors<\/jats:ext-link>\n                      <\/jats:p>\n                    <\/jats:list-item>\n                  <\/jats:list>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>CONTACT<\/jats:title>\n                  <jats:p>\n                    The Review Commons office can be contacted directly at:\n                    <jats:email>office@reviewcommons.org<\/jats:email>\n                  <\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2024.04.30.591837","type":"posted-content","created":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T13:45:43Z","timestamp":1714484743000},"source":"Crossref","is-referenced-by-count":3,"title":["Reversible host cell surface remodelling limits immune recognition and maximizes transmission of\n                  <i>Plasmodium falciparum<\/i>\n                  gametocytes"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3140-5847","authenticated-orcid":false,"given":"Priscilla","family":"Ngotho","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen Dantzler","family":"Press","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan","family":"Peedell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Muasya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian Roy","family":"Omondi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanley E.","family":"Otoboh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl B.","family":"Seydel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0321-7128","authenticated-orcid":false,"given":"Melissa","family":"Kapulu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Laufer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7416-3986","authenticated-orcid":false,"given":"Terrie","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2666-094X","authenticated-orcid":false,"given":"Teun","family":"Bousema","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1040-9566","authenticated-orcid":false,"given":"Matthias","family":"Marti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2024050309200447000_2024.04.30.591837v1.1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2012.01786.x"},{"key":"2024050309200447000_2024.04.30.591837v1.2","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1186\/1475-2875-7-101","article-title":"A 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