{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T09:40:10Z","timestamp":1770543610481,"version":"3.49.0"},"posted":{"date-parts":[[2024,5,6]]},"group-title":"Biophysics","reference-count":52,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,5,6]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                <jats:p>Binding of autoantibodies to keratinocyte surface antigens, primarily desmoglein 3 (Dsg3) of the desmosomal complex, leads to the dissociation of cell-cell adhesion in the blistering disorder pemphigus vulgaris (PV). After the initial disassembly of desmosomes, cell-cell adhesions actively remodel in association with the cytoskeleton and focal adhesions. Growing evidence highlights the role of adhesion mechanics and mechanotransduction at cell-cell adhesions in this remodeling process, as their active participation may direct autoimmune pathogenicity. However, a large part of the biophysical transformations after antibody binding remains underexplored. Specifically, it is unclear how tension in desmosomes and cell-cell adhesions changes in response to antibodies, and how the altered tensional states translate to cellular responses. Here, we showed a tension loss at Dsg3 using fluorescence resonance energy transfer (FRET)-based tension sensors, a tension loss at the entire cell-cell adhesion, and a potentially compensatory increase in junctional traction force at cell-extracellular matrix adhesions after PV antibody binding. Further, our data indicate that this tension loss is mediated by the inhibition of RhoA at cell-cell contacts, and the extent of RhoA inhibition may be crucial in determining the severity of pathogenicity among different PV antibodies. More importantly, this tension loss can be partially restored by altering actomyosin based cell contractility. Collectively, these findings provide previously unattainable details in our understanding of the mechanisms that govern cell-cell interactions under physiological and autoimmune conditions, which may open the window to entirely new therapeutics aimed at restoring physiological balance to tension dynamics that regulates the maintenance of cell-cell adhesion.<\/jats:p>","DOI":"10.1101\/2024.05.03.592394","type":"posted-content","created":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T14:50:18Z","timestamp":1715007018000},"source":"Crossref","is-referenced-by-count":3,"title":["Desmosomal Cadherin Tension Loss in Pemphigus Vulgaris Mediated by the Inhibition of Active RhoA at Cell-Cell Adhesions"],"prefix":"10.64898","author":[{"given":"Xiaowei","family":"Jin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jordan","family":"Rosenbohm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amir Ostadi","family":"Moghaddam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eunju","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristina","family":"Seiffert-Sinha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Merced","family":"Leiker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiwei","family":"Zhai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sindora R.","family":"Baddam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grayson","family":"Minnick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yucheng","family":"Huo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bahareh Tajvidi","family":"Safa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"James K.","family":"Wahl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fanben","family":"Meng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changjin","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung Yul","family":"Lim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel E.","family":"Conway","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Animesh A.","family":"Sinha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruiguo","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024050904200787000_2024.05.03.592394v1.1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00692"},{"key":"2024050904200787000_2024.05.03.592394v1.2","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.3389\/fimmu.2019.01418","article-title":"Pemphigus: current and future therapeutic strategies","volume":"10","year":"2019","journal-title":"Frontiers in immunology"},{"key":"2024050904200787000_2024.05.03.592394v1.3","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf6756"},{"key":"2024050904200787000_2024.05.03.592394v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5701260"},{"key":"2024050904200787000_2024.05.03.592394v1.5","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200605125"},{"key":"2024050904200787000_2024.05.03.592394v1.6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0101824"},{"key":"2024050904200787000_2024.05.03.592394v1.7","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1038\/s41467-022-35741-0","article-title":"Apremilast prevents blistering in human epidermis and stabilizes keratinocyte adhesion in pemphigus","volume":"14","year":"2023","journal-title":"nature communications"},{"key":"2024050904200787000_2024.05.03.592394v1.8","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.228031"},{"key":"2024050904200787000_2024.05.03.592394v1.9","doi-asserted-by":"crossref","first-page":"884067","DOI":"10.3389\/fimmu.2022.884067","article-title":"Mechanisms causing acantholysis in pemphigus-lessons from human skin","volume":"13","year":"2022","journal-title":"Front. 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