{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T21:16:54Z","timestamp":1768511814576,"version":"3.49.0"},"posted":{"date-parts":[[2024,5,9]]},"group-title":"Microbiology","reference-count":47,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,5,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  <jats:italic>Streptococcus mutans,<\/jats:italic>\n                  the causative agent of human dental caries, expresses a cell wall attached Serotype\n                  <jats:italic>c-<\/jats:italic>\n                  specific Carbohydrate (SCC) that is critical for cell viability. SCC consists of a repeating \u21923)\u03b1-Rha(1\u21922)\u03b1-Rha(1\u2192 polyrhamnose backbone, with glucose (Glc) side-chains and glycerol phosphate (GroP) decorations. This study reveals that SCC has one major and two minor Glc modifications. The major Glc modification, \u03b1-Glc, attached to position 2 of 3-rhamnose, is installed by SccN and SccM glycosyltransferases and is the site of the GroP addition. The minor Glc modifications are \u03b2-Glc linked to position 4 of 3-rhamnose installed by SccP and SccQ glycosyltransferases, and \u03b1-Glc attached to position 4 of 2-rhamnose installed by SccN working in tandem with an unknown enzyme. Both the major and the minor \u03b2-Glc modifications control bacterial morphology, but only the GroP and major Glc modifications are critical for biofilm formation.\n                <\/jats:p>","DOI":"10.1101\/2024.05.09.593426","type":"posted-content","created":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T01:20:13Z","timestamp":1715304013000},"source":"Crossref","is-referenced-by-count":1,"title":["Structure and mechanism of biosynthesis of\n                  <i>Streptococcus mutans<\/i>\n                  cell wall polysaccharide"],"prefix":"10.64898","author":[{"given":"Jeffrey S.","family":"Rush","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9971-4211","authenticated-orcid":false,"given":"Svetlana","family":"Zamakhaeva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7263-7193","authenticated-orcid":false,"given":"Nicholas R.","family":"Murner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2974-7389","authenticated-orcid":false,"given":"Pan","family":"Deng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1910-4865","authenticated-orcid":false,"given":"Andrew J.","family":"Morris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cameron W.","family":"Kenner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7075-7517","authenticated-orcid":false,"given":"Ian","family":"Black","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1118-8953","authenticated-orcid":false,"given":"Christian","family":"Heiss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6166-9432","authenticated-orcid":false,"given":"Parastoo","family":"Azadi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2182-6843","authenticated-orcid":false,"given":"Konstantin V.","family":"Korotkov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8303-4481","authenticated-orcid":false,"given":"G\u00f6ran","family":"Widmalm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8696-4892","authenticated-orcid":false,"given":"Natalia","family":"Korotkova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024051313250718000_2024.05.09.593426v1.1","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuw006"},{"key":"2024051313250718000_2024.05.09.593426v1.2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002756"},{"key":"2024051313250718000_2024.05.09.593426v1.3","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1038\/s41589-019-0251-4","article-title":"Discovery of glycerol phosphate modification on streptococcal rhamnose polysaccharides","volume":"15","year":"2019","journal-title":"Nature chemical biology"},{"key":"2024051313250718000_2024.05.09.593426v1.4","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1038\/s41589-021-00803-9","article-title":"Modification of cell wall polysaccharide guides cell division in Streptococcus mutans","volume":"17","year":"2021","journal-title":"Nature chemical biology"},{"key":"2024051313250718000_2024.05.09.593426v1.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.09.018"},{"key":"2024051313250718000_2024.05.09.593426v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.05.009"},{"key":"2024051313250718000_2024.05.09.593426v1.7","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s10719-017-9798-z","article-title":"Investigating the candidacy of the serotype specific rhamnan polysaccharide based glycoconjugates to prevent disease caused by the dental pathogen Streptococcus mutans","volume":"35","year":"2018","journal-title":"Glycoconjugate journal"},{"key":"2024051313250718000_2024.05.09.593426v1.8","doi-asserted-by":"publisher","DOI":"10.1086\/498618"},{"key":"2024051313250718000_2024.05.09.593426v1.9","doi-asserted-by":"crossref","unstructured":"Di Benedetto R , et al. 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