{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T05:47:14Z","timestamp":1782280034247,"version":"3.54.5"},"posted":{"date-parts":[[2024,5,12]]},"group-title":"Developmental Biology","reference-count":89,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,5,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Vitamin D is a hormone regulating gene transcription. Prenatal vitamin D has been linked to immune and vascular function in the placenta, a key organ of pregnancy. To date, studies of vitamin D and placental gene expression have focused on a limited number of candidate genes. Transcriptome-wide RNA sequencing can provide a more complete representation of the placental effects of vitamin D.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>We investigated the association between prenatal vitamin D levels and placental gene expression in a large, prospective pregnancy cohort.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Participants were recruited in Shelby County, Tennessee in the Conditions Affecting Neurocognitive Development and Learning in Early childhood (CANDLE) study. Vitamin D level (plasma total 25-hydroxyvitatmin D, [25(OH)D]) was measured at mid-pregnancy (16-28 weeks\u2019 gestation) and delivery. Placenta samples were collected at birth. RNA was isolated and sequenced. We identified differentially expressed genes (DEGs) using adjusted linear regression models. We also conducted weighted gene co-expression network analysis (WGCNA).<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>\n                    The median 25(OH)D of participants was 21.8 ng\/mL at mid-pregnancy (\n                    <jats:italic>N<\/jats:italic>\n                    =774, IQR: 15.4-26.5 ng\/mL) and 23.6 ng\/mL at delivery (\n                    <jats:italic>N<\/jats:italic>\n                    =753, IQR: 16.8-29.1 ng\/mL). Placental expression of 25 DEGs was associated with 25(OH)D at mid-pregnancy, but no DEG was associated with 25(OH)D at delivery. DEGs were related to energy metabolism, cytoskeletal function, and RNA transcription. Using WGCNA, we identified 2 gene modules whose expression was associated with 25(OH)D at mid-pregnancy and 1 module associated with 25(OH)D at delivery. These modules were enriched for genes related to mitochondrial and cytoskeletal function, and were regulated by transcription factors including\n                    <jats:italic>ARNT2<\/jats:italic>\n                    ,\n                    <jats:italic>BHLHE40<\/jats:italic>\n                    ,\n                    <jats:italic>FOSL2<\/jats:italic>\n                    ,\n                    <jats:italic>JUND<\/jats:italic>\n                    , and\n                    <jats:italic>NFKB1<\/jats:italic>\n                    .\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Our results indicate that 25(OH)D during mid-pregnancy, but not at delivery, is associated with placental gene expression at birth. Future research is needed to investigate a potential role of vitamin D in programming placental mitochondrial metabolism, intracellular transport, and transcriptional regulation during pregnancy.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2024.05.10.593571","type":"posted-content","created":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T13:50:12Z","timestamp":1715521812000},"source":"Crossref","is-referenced-by-count":1,"title":["Associations Between Prenatal Vitamin D and Placental Gene Expression"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4052-176X","authenticated-orcid":false,"given":"Mariana","family":"Parenti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa M.","family":"Melough","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha","family":"Lapehn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"MacDonald","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theo","family":"Bammler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evan J.","family":"Firsick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyo Young","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen J.","family":"Derefinko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel A.","family":"Enquobahrie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kecia N.","family":"Carroll","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaja Z.","family":"LeWinn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole R.","family":"Bush","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheela","family":"Sathyanarayana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison G.","family":"Paquette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2024051512051432000_2024.05.10.593571v1.1","doi-asserted-by":"crossref","unstructured":"Wagner CL , Hollis BW. The Implications of Vitamin D Status During Pregnancy on Mother and her Developing Child. Frontiers in Endocrinology [Internet] Frontiers Media SA; 2018 [cited 2024 Feb 15];9. Available from: https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6127214\/","DOI":"10.3389\/fendo.2018.00500"},{"key":"2024051512051432000_2024.05.10.593571v1.2","doi-asserted-by":"crossref","unstructured":"Wilson RL , Leviton AJ , Leemaqz SY , Anderson PH , Grieger JA , Grzeskowiak LE , Verburg PE , McCowan L , Dekker GA , Bianco-Miotto T , et al. Vitamin D levels in an Australian and New Zealand cohort and the association with pregnancy outcome. BMC Pregnancy and Childbirth [Internet] BMC; 2018 [cited 2024 Feb 16];18. 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