{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T14:10:41Z","timestamp":1776003041287,"version":"3.50.1"},"posted":{"date-parts":[[2024,5,14]]},"group-title":"Biochemistry","reference-count":52,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,5,14]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  <jats:italic>Capsaspora owczarzaki<\/jats:italic>\n                  is a protozoan that may both reveal aspects of animal evolution and also curtail the spread of schistosomiasis, a neglected tropical disease.\n                  <jats:italic>Capsaspora<\/jats:italic>\n                  exhibits a chemically regulated aggregative behavior that resembles cellular aggregation in some animals. This behavior may have played a key role in the evolution of animal multicellularity. Additionally, this aggregative behavior may be important for\n                  <jats:italic>Capsaspora<\/jats:italic>\n                  \u2019s ability to colonize the intermediate host of parasitic schistosomes and potentially prevent the spread of schistosomiasis. Both applications demand elucidation of the molecular mechanism of\n                  <jats:italic>Capsaspora<\/jats:italic>\n                  aggregation. Toward this goal, we first determined the necessary chemical properties of lipid cues that activate aggregation. We found that a wide range of abundant zwitterionic lipids induced aggregation, revealing that the aggregative behavior could be activated by diverse lipid-rich conditions. Furthermore, we demonstrated that aggregation in\n                  <jats:italic>Capsaspora<\/jats:italic>\n                  requires clathrin-mediated endocytosis, highlighting the potential significance of endocytosis-linked cellular signaling in recent animal ancestors. Finally, we found that aggregation was initiated by post-translational activation of cell-cell adhesion\u2014not transcriptional regulation of cellular adhesion machinery. Our findings illuminate the chemical, molecular and cellular mechanisms that regulate\n                  <jats:italic>Capsaspora<\/jats:italic>\n                  aggregative behavior\u2014with implications for the evolution of animal multicellularity and the transmission of parasites.\n                <\/jats:p>","DOI":"10.1101\/2024.05.14.593945","type":"posted-content","created":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T01:05:20Z","timestamp":1715735120000},"source":"Crossref","is-referenced-by-count":2,"title":["Exogenous lipid vesicles induce endocytosis-mediated cellular aggregation in a close unicellular relative of animals"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2518-2811","authenticated-orcid":false,"given":"Ria Q.","family":"Kidner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eleanor B.","family":"Goldstone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry J.","family":"Rodefeld","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorin P.","family":"Brokaw","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aria M.","family":"Gonzalez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0897-0186","authenticated-orcid":false,"given":"N\u00faria","family":"Ros-Rocher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0375-4110","authenticated-orcid":false,"given":"Joseph P.","family":"Gerdt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024051808350423000_2024.05.14.593945v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.03.034"},{"key":"2024051808350423000_2024.05.14.593945v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3325"},{"key":"2024051808350423000_2024.05.14.593945v1.3","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2011(79)90149-6"},{"key":"2024051808350423000_2024.05.14.593945v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(02)00066-8"},{"key":"2024051808350423000_2024.05.14.593945v1.5","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2011(80)90079-8"},{"key":"2024051808350423000_2024.05.14.593945v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2017.05.074"},{"key":"2024051808350423000_2024.05.14.593945v1.7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2216668120"},{"key":"2024051808350423000_2024.05.14.593945v1.8","doi-asserted-by":"publisher","DOI":"10.1101\/2024.01.31.578218"},{"key":"2024051808350423000_2024.05.14.593945v1.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2017.09.016"},{"key":"2024051808350423000_2024.05.14.593945v1.10","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-032421-120023"},{"key":"2024051808350423000_2024.05.14.593945v1.11","doi-asserted-by":"publisher","DOI":"10.3390\/genes12040506"},{"key":"2024051808350423000_2024.05.14.593945v1.12","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-019-0349-7"},{"key":"2024051808350423000_2024.05.14.593945v1.13","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2012.10.028"},{"key":"2024051808350423000_2024.05.14.593945v1.14","doi-asserted-by":"publisher","DOI":"10.1007\/BF00910738"},{"key":"2024051808350423000_2024.05.14.593945v1.15","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.08.005"},{"key":"2024051808350423000_2024.05.14.593945v1.16","doi-asserted-by":"publisher","DOI":"10.1101\/2024.03.25.586565"},{"key":"2024051808350423000_2024.05.14.593945v1.17","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2016.09.019"},{"key":"2024051808350423000_2024.05.14.593945v1.18","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.R036095"},{"key":"2024051808350423000_2024.05.14.593945v1.19","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2018.07.012"},{"key":"2024051808350423000_2024.05.14.593945v1.20","doi-asserted-by":"crossref","unstructured":"Marsh D . 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