{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T20:49:35Z","timestamp":1768510175593,"version":"3.49.0"},"posted":{"date-parts":[[2024,5,21]]},"group-title":"Developmental Biology","reference-count":74,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,5,21]],"date-time":"2024-05-21T00:00:00Z","timestamp":1716249600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,3,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  The trigeminal ganglion emerges from the condensation of two distinct precursor cell populations, cranial placodes and neural crest. While its dual cellular origin is well understood, the molecules underlying its formation remain relatively obscure. Trigeminal ganglion assembly is mediated, in part, by neural cadherin (N-cadherin), which is initially expressed by placodal neurons and required for their proper coalescence with neural crest cells. Axon outgrowth first occurs from placodal neurons, but as gangliogenesis proceeds, neural crest cells also differentiate into N-cadherin-expressing neurons, and both extend axons toward targets. However, the role of N-cadherin in axon outgrowth and target innervation has not been explored. Our data show that N-cadherin knockdown in chick trigeminal placode cells decreases trigeminal ganglion size, nerve growth, and target innervation\n                  <jats:italic>in vivo<\/jats:italic>\n                  , and reduces neurite complexity of neural crest-derived neurons\n                  <jats:italic>in vitro.<\/jats:italic>\n                  Furthermore, blocking N-cadherin-mediated adhesion prevents axon extension in most placodal neurons\n                  <jats:italic>in vitro<\/jats:italic>\n                  . Collectively, these findings reveal cell- and non-cell autonomous functions for N-cadherin, highlighting its critical role in mediating reciprocal interactions between neural crest- and placode-derived neurons throughout trigeminal ganglion development.\n                <\/jats:p>","DOI":"10.1101\/2024.05.20.594965","type":"posted-content","created":{"date-parts":[[2024,5,21]],"date-time":"2024-05-21T15:30:12Z","timestamp":1716305412000},"source":"Crossref","is-referenced-by-count":2,"title":["N-cadherin facilitates trigeminal sensory neuron outgrowth and target tissue innervation"],"prefix":"10.64898","author":[{"given":"Caroline A.","family":"Halmi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carrie E.","family":"Leonard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7764-3608","authenticated-orcid":false,"given":"Alec 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