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In detecting intracellular H\n                  <jats:sub>2<\/jats:sub>\n                  S, 4-azido-(\u03b1-CF\n                  <jats:sub>3<\/jats:sub>\n                  )-benzyl resorufin offers significantly faster signal generation and improved sensitivity compared to 4-azidobenzyl resorufin. Computed free energy profiles for the 1,6-elimination process support the hypothesis that a benzylic CF\n                  <jats:sub>3<\/jats:sub>\n                  group can reduce the activation energy barrier toward probe activation. This novel probe design allows for near-real-time detection of H\n                  <jats:sub>2<\/jats:sub>\n                  S in HeLa cells under stimulation conditions.\n                <\/jats:p>","DOI":"10.1101\/2024.05.30.596105","type":"posted-content","created":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T14:25:16Z","timestamp":1717251916000},"source":"Crossref","is-referenced-by-count":0,"title":["Benzylic Trifluoromethyl Accelerates 1,6-Elimination Toward Rapid Probe 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