{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T20:41:49Z","timestamp":1782160909037,"version":"3.54.5"},"posted":{"date-parts":[[2024,6,1]]},"group-title":"Synthetic Biology","reference-count":38,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,5,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  An\n                  <jats:italic>in vitro<\/jats:italic>\n                  model of human meiosis would accelerate research into this important reproductive process and development of therapies for infertility. We have developed a method to induce meiosis starting from male or female human pluripotent stem cells. We demonstrate that DNMT1 inhibition, retinoid signaling activation, and overexpression of regulatory factors (anti-apoptotic BCL2, and pro-meiotic HOXB5, BOLL, or MEIOC) rapidly activates meiosis, with leptonema beginning at 6 days, zygonema at 9 days, and pachynema at 12 days. Immunofluorescence microscopy shows key aspects of meiosis, including chromosome synapsis and sex body formation. The meiotic cells express genes similar to meiotic oogonia\n                  <jats:italic>in vivo<\/jats:italic>\n                  , including all synaptonemal complex components and machinery for meiotic recombination. These findings establish an accessible system for inducing human meiosis\n                  <jats:italic>in vitro<\/jats:italic>\n                  .\n                <\/jats:p>","DOI":"10.1101\/2024.05.31.596483","type":"posted-content","created":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T14:10:14Z","timestamp":1717251014000},"source":"Crossref","is-referenced-by-count":2,"title":["Induction of Meiosis from Human Pluripotent Stem Cells"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5816-7098","authenticated-orcid":false,"given":"Merrick","family":"Pierson Smela","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Adams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7702-5749","authenticated-orcid":false,"given":"Laura","family":"Breimann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ursula","family":"Widocki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshi","family":"Shioda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6232-9969","authenticated-orcid":false,"given":"George M.","family":"Church","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2024060313001497000_2024.05.31.596483v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2016.07.019"},{"key":"2024060313001497000_2024.05.31.596483v1.2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201796875"},{"key":"2024060313001497000_2024.05.31.596483v1.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2016.01.017"},{"key":"2024060313001497000_2024.05.31.596483v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/nature20104"},{"key":"2024060313001497000_2024.05.31.596483v1.5","doi-asserted-by":"crossref","unstructured":"A. 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