{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T21:40:08Z","timestamp":1768513208644,"version":"3.49.0"},"posted":{"date-parts":[[2024,6,25]]},"group-title":"Immunology","reference-count":55,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,6,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Ebolavirus disease (EVD) is caused by multiple species of\n                  <jats:italic>Ebolavirus<\/jats:italic>\n                  . Monoclonal antibodies (mAbs) against the virus glycoprotein (GP) are the only class of therapeutic approved for treatment of EVD caused by\n                  <jats:italic>Zaire ebolavirus<\/jats:italic>\n                  (EBOV). Therefore, mAbs targeting multiple\n                  <jats:italic>Ebolavirus<\/jats:italic>\n                  species may represent the next generation of EVD therapeutics. Broadly reactive anti-GP mAbs were produced; among these, mAbs 11886 and 11883 were broadly neutralizing\n                  <jats:italic>in vitro<\/jats:italic>\n                  . A 3.0 \u00c5 cryo-electron microscopy structure of EBOV GP bound to both mAbs shows that 11886 binds a novel epitope bridging the glycan cap (GC), 3\n                  <jats:sub>10<\/jats:sub>\n                  pocket and GP2 N-terminus, whereas 11883 binds the receptor binding region (RBR) and GC.\n                  <jats:italic>In vitro<\/jats:italic>\n                  , 11886 synergized with a range of mAbs with epitope specificities spanning the RBR\/GC, including 11883. Notably, 11886 increased the breadth of neutralization by partner mAbs against different\n                  <jats:italic>Ebolavirus<\/jats:italic>\n                  species. These data provide a strategic route to design improved mAb-based next-generation EVD therapeutics.\n                <\/jats:p>","DOI":"10.1101\/2024.06.21.600001","type":"posted-content","created":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T18:15:12Z","timestamp":1719339312000},"source":"Crossref","is-referenced-by-count":0,"title":["A broadly-neutralizing antibody against\n                  <i>Ebolavirus<\/i>\n                  glycoprotein that potentiates the breadth and neutralization potency of other antibodies"],"prefix":"10.64898","author":[{"given":"Francesca R.","family":"Donnellan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vamseedhar","family":"Rayaprolu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pramila","family":"Rijal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoria","family":"O\u2019Dowd","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amar","family":"Parvate","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heather","family":"Callaway","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chitra","family":"Hariharan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dipti","family":"Parekh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sean","family":"Hui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelly","family":"Shaffer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruben Diaz","family":"Avalos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathryn","family":"Hastie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Schimanski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helena","family":"M\u00fcller-Kr\u00e4uter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Strecker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ariane","family":"Balaram","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Halfmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erica Ollmann","family":"Saphire","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel J.","family":"Lightwood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alain R.","family":"Townsend","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon J.","family":"Draper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024062615050642000_2024.06.21.600001v1.1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiw207"},{"key":"2024062615050642000_2024.06.21.600001v1.2","doi-asserted-by":"crossref","unstructured":"Formenty, P. , Hatz, C. , Le Guenno, B. , Stoll, A. , Rogenmoser, P. , and Widmer, A. 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