{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T23:34:41Z","timestamp":1791588881521,"version":"4.3.4"},"posted":{"date-parts":[[2024,6,27]]},"group-title":"Microbiology","reference-count":53,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,6,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Gut microbiome is a group of microorganisms that plays important roles in contributing to health and diseases. These bacterial compositions have been demonstrated to impact bile acids (BAs) profiles, either by directly metabolizing primary BAs to secondary BAs or indirect ways through host metabolism by influencing BAs synthesis, transportation and conjugation in liver. It has been observed sexually dimorphic gut microbiome and bile acids composition, with variations in expression levels of bile acid metabolizing genes in the liver. However, associations betweensex-specific differences in gut microbiome and BAs profiles are not well understood. This study aimed to investigate whether gut microbiome could influence BAs profiles in host in a sex-specific manner. We transplanted cecum feces of male and female C57BL\/6 mice to male mice and measured BAs concentrations in feces, serum and liver samples 7 days after fecal transplantation. We found different BAs profiles between mice with male and female gut microbiome, including altering levels and proportions of secondary BAs. We also observed varied expression levels of genes related to bile acid metabolism in the liver and distal ileum.Our results highlight sex-specific effects of gut microbiome on shaping bile acid metabolism through gut bacteria and regulation of host genes.<\/jats:p>","DOI":"10.1101\/2024.06.27.601003","type":"posted-content","created":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T01:50:13Z","timestamp":1719539413000},"source":"Crossref","is-referenced-by-count":5,"title":["Sex-specific effects of gut microbiome on shaping bile acid metabolism"],"prefix":"10.64898","author":[{"given":"Yifei","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Chi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Chung","family":"Hsiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"issue":"2","key":"2024062911051107000_2024.06.27.601003v1.1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.jchromb.2008.08.018","article-title":"Quantitative-profiling of bile acids and their conjugates in mouse liver, bile, plasma, and urine using LC\u2013MS\/MS","volume":"873","year":"2008","journal-title":"Journal of Chromatography B"},{"key":"2024062911051107000_2024.06.27.601003v1.2","first-page":"2829","article-title":"OST alpha-OST beta: a key membrane transporter of bile acids and conjugated steroids","volume":"14","year":"2009","journal-title":"Frontiers in bioscience: a journal and virtual library"},{"key":"2024062911051107000_2024.06.27.601003v1.3","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.14875"},{"issue":"1","key":"2024062911051107000_2024.06.27.601003v1.4","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.mrrev.2004.08.001","article-title":"Bile acids as carcinogens in human gastrointestinal cancers","volume":"589","year":"2005","journal-title":"Mutation Research\/Reviews in Mutation Research"},{"issue":"6","key":"2024062911051107000_2024.06.27.601003v1.5","first-page":"17","article-title":"Part 1: The human gut microbiome in health and disease","volume":"13","year":"2014","journal-title":"Integrative Medicine: A Clinician\u2019s Journal"},{"issue":"6","key":"2024062911051107000_2024.06.27.601003v1.6","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1021\/acs.chemrestox.6b00066","article-title":"Sex-specific effects of arsenic exposure on the trajectory and function of the gut microbiome","volume":"29","year":"2016","journal-title":"Chemical Research in Toxicology"},{"issue":"9","key":"2024062911051107000_2024.06.27.601003v1.7","doi-asserted-by":"crossref","first-page":"097002","DOI":"10.1289\/EHP4415","article-title":"Lipid and cholesterol homeostasis after arsenic exposure and antibiotic treatment in mice: potential role of the microbiota","volume":"127","year":"2019","journal-title":"Environmental Health Perspectives"},{"issue":"3","key":"2024062911051107000_2024.06.27.601003v1.8","first-page":"1191","article-title":"Bile acid metabolism and signaling","volume":"3","year":"2013","journal-title":"Comprehensive physiology"},{"key":"2024062911051107000_2024.06.27.601003v1.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.livres.2020.05.001"},{"key":"2024062911051107000_2024.06.27.601003v1.10","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000178994.21828.a7"},{"key":"2024062911051107000_2024.06.27.601003v1.11","unstructured":"Council, N. 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