{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T16:11:12Z","timestamp":1772208672832,"version":"3.50.1"},"posted":{"date-parts":[[2024,7,30]]},"group-title":"Microbiology","reference-count":113,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T00:00:00Z","timestamp":1722297600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,12,19]]},"abstract":"<jats:p>\n                  Group B\n                  <jats:italic>Streptococcus<\/jats:italic>\n                  (GBS) is a Gram-positive pathobiont that commonly colonizes the gastrointestinal and lower female genital tracts but can cause sepsis and pneumonia in newborns and is a leading cause of neonatal meningitis. Despite the resulting disease severity, the pathogenesis of GBS is not completely understood, especially during the early phases of infection. To investigate GBS factors necessary for blood stream survival, we performed a transposon (Tn) mutant screen in our bacteremia infection model using a GBS\n                  <jats:italic>mariner<\/jats:italic>\n                  transposon mutant library previously developed by our group. We identified significantly underrepresented mutations in 623 genes that contribute to survival in the blood, including those encoding known virulence factors such as capsule, the \u03b2-hemolysin, and inorganic metal ion transport systems. Most of the underrepresented genes have not been previously characterized or studied in GBS, including\n                  <jats:italic>gloA<\/jats:italic>\n                  and\n                  <jats:italic>gloB<\/jats:italic>\n                  , which are homologs for genes involved in methylglyoxal (MG) detoxification. MG is a byproduct of glycolysis and a highly reactive toxic aldehyde that is elevated in immune cells during infection. Here, we observed MG sensitivity across multiple GBS isolates and confirm that\n                  <jats:italic>gloA<\/jats:italic>\n                  contributes to MG tolerance and invasive GBS infection. We show specifically that\n                  <jats:italic>gloA<\/jats:italic>\n                  contributes to GBS survival in the presence of neutrophils and depleting neutrophils in mice abrogates the decreased survival and infection of the\n                  <jats:italic>gloA<\/jats:italic>\n                  mutant. The requirement of the glyoxalase pathway during GBS infection suggests that MG detoxification is important for bacterial survival during host-pathogen interactions.\n                <\/jats:p>","DOI":"10.1101\/2024.07.30.605887","type":"posted-content","created":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T00:16:04Z","timestamp":1722384964000},"source":"Crossref","is-referenced-by-count":2,"title":["Identification of Glyoxalase A in Group B\n                  <i>Streptococcus<\/i>\n                  and its contribution to methylglyoxal tolerance and virulence"],"prefix":"10.64898","author":[{"given":"Madeline S","family":"Akbari","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9346-671X","authenticated-orcid":false,"given":"Luke R","family":"Joyce","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4374-6403","authenticated-orcid":false,"given":"Brady L","family":"Spencer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda","family":"Brady","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4769-4463","authenticated-orcid":false,"given":"Kevin S.","family":"McIver","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4232-6837","authenticated-orcid":false,"given":"Kelly S","family":"Doran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025010708551114000_2024.07.30.605887v3.1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04266.x"},{"key":"2025010708551114000_2024.07.30.605887v3.2","doi-asserted-by":"publisher","DOI":"10.1001\/jamainternmed.2018.7269"},{"key":"2025010708551114000_2024.07.30.605887v3.3","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.21-0300"},{"key":"2025010708551114000_2024.07.30.605887v3.4","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(85)80342-5"},{"key":"2025010708551114000_2024.07.30.605887v3.5","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2011-3453"},{"key":"2025010708551114000_2024.07.30.605887v3.6","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix660"},{"key":"2025010708551114000_2024.07.30.605887v3.7","doi-asserted-by":"publisher","DOI":"10.1530\/RAF-21-0105"},{"key":"2025010708551114000_2024.07.30.605887v3.8","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S291511"},{"key":"2025010708551114000_2024.07.30.605887v3.9","doi-asserted-by":"publisher","DOI":"10.1038\/s41390-022-02375-3"},{"key":"2025010708551114000_2024.07.30.605887v3.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2017.05.013"},{"key":"2025010708551114000_2024.07.30.605887v3.11","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01781-22"},{"key":"2025010708551114000_2024.07.30.605887v3.12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1010121"},{"key":"2025010708551114000_2024.07.30.605887v3.13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003143"},{"key":"2025010708551114000_2024.07.30.605887v3.14","doi-asserted-by":"crossref","unstructured":"M. S. Akbari , K. S. Doran , L. R. Burcham , Metal Homeostasis in Pathogenic Streptococci. Microorganisms 10, (2022).","DOI":"10.3390\/microorganisms10081501"},{"key":"2025010708551114000_2024.07.30.605887v3.15","doi-asserted-by":"crossref","unstructured":"Y. Liu , J. Liu , Group B Streptococcus: Virulence Factors and Pathogenic Mechanism. Microorganisms 10, (2022).","DOI":"10.3390\/microorganisms10122483"},{"key":"2025010708551114000_2024.07.30.605887v3.16","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.10.3212-3219.1999"},{"key":"2025010708551114000_2024.07.30.605887v3.17","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200317335"},{"key":"2025010708551114000_2024.07.30.605887v3.18","doi-asserted-by":"publisher","DOI":"10.1086\/340818"},{"key":"2025010708551114000_2024.07.30.605887v3.19","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406143101"},{"key":"2025010708551114000_2024.07.30.605887v3.20","doi-asserted-by":"publisher","DOI":"10.1086\/428946"},{"key":"2025010708551114000_2024.07.30.605887v3.21","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.5.3096-3103.2005"},{"key":"2025010708551114000_2024.07.30.605887v3.22","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.1c00182"},{"key":"2025010708551114000_2024.07.30.605887v3.23","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.3.497"},{"key":"2025010708551114000_2024.07.30.605887v3.24","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix656"},{"key":"2025010708551114000_2024.07.30.605887v3.25","doi-asserted-by":"publisher","DOI":"10.1021\/bi00519a001"},{"key":"2025010708551114000_2024.07.30.605887v3.26","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.128.3.1278"},{"key":"2025010708551114000_2024.07.30.605887v3.27","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(89)90232-6"},{"key":"2025010708551114000_2024.07.30.605887v3.28","doi-asserted-by":"crossref","unstructured":"J. Morgenstern , M. Campos Campos , P. Nawroth , T. Fleming , The Glyoxalase System-New Insights into an Ancient Metabolism. Antioxidants (Basel) 9, (2020).","DOI":"10.3390\/antiox9100939"},{"key":"2025010708551114000_2024.07.30.605887v3.29","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00701.x"},{"key":"2025010708551114000_2024.07.30.605887v3.30","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrestox.2c00160"},{"key":"2025010708551114000_2024.07.30.605887v3.31","doi-asserted-by":"crossref","unstructured":"B. L. Spencer , A. Chatterjee , B. A. Duerkop , C. J. Baker , K. S. Doran , Complete Genome Sequence of Neonatal Clinical Group B Streptococcal Isolate CJB111. Microbiol Resour Announc 10, (2021).","DOI":"10.1128\/MRA.01268-20"},{"key":"2025010708551114000_2024.07.30.605887v3.32","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.00985-22"},{"key":"2025010708551114000_2024.07.30.605887v3.33","doi-asserted-by":"crossref","unstructured":"L. R. Burcham et al., Identification of Zinc-Dependent Mechanisms Used by Group B Streptococcus To Overcome Calprotectin-Mediated Stress. mBio 11, (2020).","DOI":"10.1128\/mBio.02302-20"},{"key":"2025010708551114000_2024.07.30.605887v3.34","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2911(08)60146-0"},{"key":"2025010708551114000_2024.07.30.605887v3.35","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03655.x"},{"key":"2025010708551114000_2024.07.30.605887v3.36","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.5.2408-2413.2002"},{"key":"2025010708551114000_2024.07.30.605887v3.37","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05190.x"},{"key":"2025010708551114000_2024.07.30.605887v3.38","doi-asserted-by":"publisher","DOI":"10.1172\/JCI23829"},{"key":"2025010708551114000_2024.07.30.605887v3.39","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20122753"},{"key":"2025010708551114000_2024.07.30.605887v3.40","doi-asserted-by":"crossref","unstructured":"E. Boldenow et al., Group B Streptococcus circumvents neutrophils and neutrophil extracellular traps during amniotic cavity invasion and preterm labor. Sci Immunol 1, (2016).","DOI":"10.1126\/sciimmunol.aah4576"},{"key":"2025010708551114000_2024.07.30.605887v3.41","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0018747"},{"key":"2025010708551114000_2024.07.30.605887v3.42","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiy341"},{"key":"2025010708551114000_2024.07.30.605887v3.43","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-45336-6"},{"key":"2025010708551114000_2024.07.30.605887v3.44","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20090691"},{"key":"2025010708551114000_2024.07.30.605887v3.45","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1815572116"},{"key":"2025010708551114000_2024.07.30.605887v3.46","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00614-16"},{"key":"2025010708551114000_2024.07.30.605887v3.47","doi-asserted-by":"crossref","unstructured":"M. S. Akbari et al., The impact of nutritional immunity on Group B streptococcal pathogenesis during wound infection. mBio, e0030423 (2023).","DOI":"10.1128\/mbio.00304-23"},{"key":"2025010708551114000_2024.07.30.605887v3.48","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04974.x"},{"key":"2025010708551114000_2024.07.30.605887v3.49","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13335"},{"key":"2025010708551114000_2024.07.30.605887v3.50","first-page":"e0031521","article-title":"Copper Intoxication in Group B Streptococcus Triggers Transcriptional Activation of the cop Operon That Contributes to Enhanced Virulence during Acute Infection","volume":"203","year":"2021","journal-title":"J Bacteriol"},{"key":"2025010708551114000_2024.07.30.605887v3.51","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00870-14"},{"key":"2025010708551114000_2024.07.30.605887v3.52","doi-asserted-by":"crossref","unstructured":"L. Thomas , L. Cook , Two-Component Signal Transduction Systems in the Human Pathogen Streptococcus agalactiae. Infect Immun 88, (2020).","DOI":"10.1128\/IAI.00931-19"},{"key":"2025010708551114000_2024.07.30.605887v3.53","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00938-15"},{"key":"2025010708551114000_2024.07.30.605887v3.54","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.21.6324-6334.2001"},{"key":"2025010708551114000_2024.07.30.605887v3.55","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01216-08"},{"key":"2025010708551114000_2024.07.30.605887v3.56","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00010"},{"key":"2025010708551114000_2024.07.30.605887v3.57","doi-asserted-by":"publisher","DOI":"10.1515\/hsz-2013-0167"},{"key":"2025010708551114000_2024.07.30.605887v3.58","doi-asserted-by":"publisher","DOI":"10.1038\/srep18679"},{"key":"2025010708551114000_2024.07.30.605887v3.59","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04620.x"},{"key":"2025010708551114000_2024.07.30.605887v3.60","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.6.2877-2885.2002"},{"key":"2025010708551114000_2024.07.30.605887v3.61","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-021-00934-w"},{"key":"2025010708551114000_2024.07.30.605887v3.62","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00370-09"},{"key":"2025010708551114000_2024.07.30.605887v3.63","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2011.03.001"},{"key":"2025010708551114000_2024.07.30.605887v3.64","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.27.2.444-448.1980"},{"key":"2025010708551114000_2024.07.30.605887v3.65","doi-asserted-by":"crossref","unstructured":"M. M. Zhang , C. L. Ong , M. J. Walker , A. G. McEwan , Defence against methylglyoxal in Group A Streptococcus: a role for Glyoxylase I in bacterial virulence and survival in neutrophils? Pathog Dis 74, (2016).","DOI":"10.1093\/femspd\/ftv122"},{"key":"2025010708551114000_2024.07.30.605887v3.66","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009819"},{"key":"2025010708551114000_2024.07.30.605887v3.67","doi-asserted-by":"publisher","DOI":"10.1021\/bi000856g"},{"key":"2025010708551114000_2024.07.30.605887v3.68","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.70"},{"key":"2025010708551114000_2024.07.30.605887v3.69","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.220010"},{"key":"2025010708551114000_2024.07.30.605887v3.70","first-page":"e0036323","article-title":"Aldehyde accumulation in Mycobacterium tuberculosis with defective proteasomal degradation results in copper sensitivity","volume":"14","year":"2023","journal-title":"mBio"},{"key":"2025010708551114000_2024.07.30.605887v3.71","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000029"},{"key":"2025010708551114000_2024.07.30.605887v3.72","doi-asserted-by":"publisher","DOI":"10.4110\/in.2020.20.e46"},{"key":"2025010708551114000_2024.07.30.605887v3.73","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0407247"},{"key":"2025010708551114000_2024.07.30.605887v3.74","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200005183422007"},{"key":"2025010708551114000_2024.07.30.605887v3.75","doi-asserted-by":"crossref","unstructured":"T. A. Hooven et al., The Streptococcus agalactiae Stringent Response Enhances Virulence and Persistence in Human Blood. Infect Immun 86, (2018).","DOI":"10.1128\/IAI.00612-17"},{"key":"2025010708551114000_2024.07.30.605887v3.76","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007145"},{"key":"2025010708551114000_2024.07.30.605887v3.77","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061294"},{"key":"2025010708551114000_2024.07.30.605887v3.78","doi-asserted-by":"publisher","DOI":"10.1016\/j.eimc.2017.08.006"},{"key":"2025010708551114000_2024.07.30.605887v3.79","doi-asserted-by":"crossref","first-page":"166425","DOI":"10.1016\/j.bbadis.2022.166425","article-title":"Immunometabolism and the modulation of immune responses and host defense: A role for methylglyoxal?","volume":"1868","year":"2022","journal-title":"Biochim Biophys Acta Mol Basis Dis"},{"key":"2025010708551114000_2024.07.30.605887v3.80","doi-asserted-by":"publisher","DOI":"10.1042\/BST20130275"},{"key":"2025010708551114000_2024.07.30.605887v3.81","doi-asserted-by":"crossref","unstructured":"C. Lee , C. Park , Bacterial Responses to Glyoxal and Methylglyoxal: Reactive Electrophilic Species. Int J Mol Sci 18, (2017).","DOI":"10.3390\/ijms18010169"},{"key":"2025010708551114000_2024.07.30.605887v3.82","doi-asserted-by":"publisher","DOI":"10.1021\/bi972449j"},{"key":"2025010708551114000_2024.07.30.605887v3.83","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.820365"},{"key":"2025010708551114000_2024.07.30.605887v3.84","doi-asserted-by":"publisher","DOI":"10.1042\/bj20030271"},{"key":"2025010708551114000_2024.07.30.605887v3.85","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.251603"},{"key":"2025010708551114000_2024.07.30.605887v3.86","doi-asserted-by":"publisher","DOI":"10.1080\/20002297.2024.2322241"},{"key":"2025010708551114000_2024.07.30.605887v3.87","doi-asserted-by":"crossref","unstructured":"L. C. C. Cook , H. Hu , M. Maienschein-Cline , M. J. Federle , A Vaginal Tract Signal Detected by the Group B Streptococcus SaeRS System Elicits Transcriptomic Changes and Enhances Murine Colonization. Infect Immun 86, (2018).","DOI":"10.1128\/IAI.00762-17"},{"key":"2025010708551114000_2024.07.30.605887v3.88","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3836"},{"key":"2025010708551114000_2024.07.30.605887v3.89","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050001"},{"key":"2025010708551114000_2024.07.30.605887v3.90","doi-asserted-by":"publisher","DOI":"10.1128\/jb.108.3.1338-1347.1971"},{"key":"2025010708551114000_2024.07.30.605887v3.91","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a131472"},{"key":"2025010708551114000_2024.07.30.605887v3.92","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a131471"},{"key":"2025010708551114000_2024.07.30.605887v3.93","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(79)90529-0"},{"key":"2025010708551114000_2024.07.30.605887v3.94","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2014.129"},{"key":"2025010708551114000_2024.07.30.605887v3.95","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00001.2019"},{"key":"2025010708551114000_2024.07.30.605887v3.96","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.add3221"},{"key":"2025010708551114000_2024.07.30.605887v3.97","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1011441"},{"key":"2025010708551114000_2024.07.30.605887v3.98","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3001555"},{"key":"2025010708551114000_2024.07.30.605887v3.99","doi-asserted-by":"crossref","unstructured":"A. Banerjee et al., Bacterial Pili exploit integrin machinery to promote immune activation and efficient blood-brain barrier penetration. Nat Commun 2, 462 (2011).","DOI":"10.1038\/ncomms1474"},{"key":"2025010708551114000_2024.07.30.605887v3.100","doi-asserted-by":"publisher","DOI":"10.1172\/JCI74159"},{"key":"2025010708551114000_2024.07.30.605887v3.101","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01930"},{"key":"2025010708551114000_2024.07.30.605887v3.102","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.12688\/f1000research.15931.1","article-title":"FastQ Screen: A tool for multi-genome mapping and quality control","volume":"7","year":"2018","journal-title":"F1000Res"},{"key":"2025010708551114000_2024.07.30.605887v3.103","doi-asserted-by":"publisher","DOI":"10.14806\/ej.17.1.200"},{"key":"2025010708551114000_2024.07.30.605887v3.104","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1004401"},{"key":"2025010708551114000_2024.07.30.605887v3.105","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp324"},{"key":"2025010708551114000_2024.07.30.605887v3.106","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac240"},{"key":"2025010708551114000_2024.07.30.605887v3.107","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku316"},{"key":"2025010708551114000_2024.07.30.605887v3.108","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"2025010708551114000_2024.07.30.605887v3.109","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2005.04945.x"},{"key":"2025010708551114000_2024.07.30.605887v3.110","doi-asserted-by":"publisher","DOI":"10.1038\/S41586-021-03819-2"},{"key":"2025010708551114000_2024.07.30.605887v3.111","doi-asserted-by":"publisher","DOI":"10.1038\/S41592-022-01488-1"},{"key":"2025010708551114000_2024.07.30.605887v3.112","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.235"},{"key":"2025010708551114000_2024.07.30.605887v3.113","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adn7848"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2024.07.30.605887","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T06:47:33Z","timestamp":1768459653000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2024.07.30.605887"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,30]]},"references-count":113,"URL":"https:\/\/doi.org\/10.1101\/2024.07.30.605887","relation":{"is-preprint-of":[{"id-type":"doi","id":"10.1128\/iai.00540-24","asserted-by":"subject"}]},"subject":[],"published":{"date-parts":[[2024,7,30]]},"subtype":"preprint"}}