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The temporal and spatial regulation of Ca\u00b2\u207a is crucial for the precise activation of key biological processes. In the apicomplexan parasite\n                  <jats:italic>Toxoplasma gondii<\/jats:italic>\n                  , which infects approximately one-third of the global population, Ca\u00b2\u207a signaling governs essential aspects of the parasite\u2019s infection cycle.\n                  <jats:italic>T. gondii<\/jats:italic>\n                  relies on Ca\u00b2\u207a signals to regulate pathogenic traits, with several Ca\u00b2\u207a-signaling components playing critical roles. Ca\n                  <jats:sup>2+<\/jats:sup>\n                  entry from the extracellular environment has been demonstrated in\n                  <jats:italic>T. gondii<\/jats:italic>\n                  for both, extracellular parasites, exposed to high Ca\n                  <jats:sup>2+<\/jats:sup>\n                  , and intracellular parasites, which acquire Ca\u00b2\u207a from host cells during host Ca\u00b2\u207a signaling events. Active egress, an essential step of the parasite\u2019s infection cycle, is preceded by a large increase in cytosolic Ca\n                  <jats:sup>2+<\/jats:sup>\n                  , most likely initiated by release from intracellular stores. However, extracellular Ca\n                  <jats:sup>2+<\/jats:sup>\n                  is also necessary to reach a cytosolic Ca\n                  <jats:sup>2+<\/jats:sup>\n                  threshold required for timely egress. In this study, we investigated the mechanism of Ca\u00b2\u207a intracellular store replenishment and identified a central role for the SERCA-Ca\n                  <jats:sup>2+<\/jats:sup>\n                  -ATPase in maintaining Ca\u00b2\u207a homeostasis not only within the ER but also in other organelles. We demonstrate mitochondrial Ca\n                  <jats:sup>2+<\/jats:sup>\n                  uptake, which occurs by transfer of Ca\n                  <jats:sup>2+<\/jats:sup>\n                  from the ER, likely through membrane contact sites. Our findings suggest that the\n                  <jats:italic>T. gondii<\/jats:italic>\n                  ER plays a key role in sequestering and redistributing Ca\u00b2\u207a to intracellular organelles following Ca\u00b2\u207a influx at the PM.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>HIGHLIGHTS<\/jats:title>\n                  <jats:p>\n                    The\n                    <jats:italic>T. gondii<\/jats:italic>\n                    ER takes up Ca\n                    <jats:sup>2+<\/jats:sup>\n                    that enters the cytosol from the extracellular milieu.\n                  <\/jats:p>\n                  <jats:p>\n                    Filling of acidic stores in\n                    <jats:italic>T. gondii<\/jats:italic>\n                    depends on ER Ca\u00b2\u207a content.\n                  <\/jats:p>\n                  <jats:p>\n                    The mitochondrion of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    has no direct access to extracellular Ca\u00b2\u207a but can take it up via transfer from the ER and\/or acidic stores.\n                  <\/jats:p>\n                  <jats:p>The absence of SERCA activity results in reduced Ca\u00b2\u207a levels in the ER as well as in other organelles<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2024.08.15.608087","type":"posted-content","created":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T04:50:15Z","timestamp":1723783815000},"source":"Crossref","is-referenced-by-count":3,"title":["Calcium transfer from the ER to other organelles for optimal signaling in\n                  <i>Toxoplasma gondii<\/i>"],"prefix":"10.64898","author":[{"given":"Zhu-Hong","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beejan","family":"Asady","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miryam Andrea","family":"Hortua Triana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Coppens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2041-6295","authenticated-orcid":false,"given":"Silvia N.J.","family":"Moreno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025100604400512000_2024.08.15.608087v3.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2003.11.017"},{"key":"2025100604400512000_2024.08.15.608087v3.2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200309097"},{"key":"2025100604400512000_2024.08.15.608087v3.3","doi-asserted-by":"crossref","unstructured":"Atakpa, P. , van Marrewijk, L.M. , Apta-Smith, M. , Chakraborty, S. , and Taylor, C.W. 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