{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T22:09:58Z","timestamp":1768514998994,"version":"3.49.0"},"posted":{"date-parts":[[2024,8,19]]},"group-title":"Immunology","reference-count":0,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T00:00:00Z","timestamp":1724025600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,8,19]]},"abstract":"<jats:p>\n                  <jats:italic>Candida albicans<\/jats:italic>\n                  is a ubiquitous fungus in the human gut microbiome as well as a prevalent cause of opportunistic mucosal and systemic disease. There is currently little understanding, however, as to how crosstalk between\n                  <jats:italic>C. albicans<\/jats:italic>\n                  and the host regulates colonization of this key niche. Here, we performed expression profiling on ileal and colonic tissues in germ-free mice colonized with\n                  <jats:italic>C. albicans<\/jats:italic>\n                  to define the global response to this fungus. We reveal that\n                  <jats:italic>Duox2<\/jats:italic>\n                  and\n                  <jats:italic>Duoxa2<\/jats:italic>\n                  , encoding dual NADPH oxidase activity, are upregulated in both the ileum and colon, and that induction requires the\n                  <jats:italic>C. albicans<\/jats:italic>\n                  yeast-hyphal transition and the hyphal-specific toxin candidalysin. Hosts lacking the IL-17 receptor failed to upregulate\n                  <jats:italic>Duox2\/Duoxa2<\/jats:italic>\n                  in response to\n                  <jats:italic>C. albicans<\/jats:italic>\n                  , while addition of IL-17A to colonoids induced these genes together with the concomitant production of hydrogen peroxide. To directly define the role of\n                  <jats:italic>Duox2\/Duoxa2<\/jats:italic>\n                  in fungal colonization, antibiotic-treated mice lacking intestinal DUOX2 activity were evaluated for\n                  <jats:italic>C. albicans<\/jats:italic>\n                  colonization and host responses. Surprisingly, loss of DUOX2 function reduced fungal colonization at extended time points (&gt;17 days colonization) and increased the proportion of hyphal cells in the gut. IL-17A levels were also elevated in\n                  <jats:italic>C. albicans<\/jats:italic>\n                  -colonized mice lacking functional DUOX2 highlighting cross-regulation between this cytokine and DUOX2. Together, these experiments reveal novel links between fungal cells, candidalysin toxin and the host IL-17-DUOX2 axis, and that a complex interplay between these factors regulates\n                  <jats:italic>C. albicans<\/jats:italic>\n                  filamentation and colonization in the gut.\n                <\/jats:p>","DOI":"10.1101\/2024.08.16.608271","type":"posted-content","created":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T14:50:19Z","timestamp":1724079019000},"source":"Crossref","is-referenced-by-count":2,"title":["An IL-17-DUOX2 axis controls gastrointestinal colonization by\n                  <i>Candida albicans<\/i>"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0388-9012","authenticated-orcid":false,"given":"Pallavi","family":"Kakade","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7641-5136","authenticated-orcid":false,"given":"Juan F","family":"Burgueno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4553-6901","authenticated-orcid":false,"given":"Shabnam","family":"Sircaik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2704-0451","authenticated-orcid":false,"given":"Nicole","family":"Ponde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinke","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0390-7084","authenticated-orcid":false,"given":"Iuliana V","family":"Ene","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiwoong","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7607-0326","authenticated-orcid":false,"given":"Shen-Huan","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9212-9340","authenticated-orcid":false,"given":"Rebecca","family":"Yunker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasutada","family":"Akiba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5377-9586","authenticated-orcid":false,"given":"Shipra","family":"Vaishnava","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6064-5973","authenticated-orcid":false,"given":"Jonathan D","family":"Kaunitz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8948-1952","authenticated-orcid":false,"given":"Sing Sing","family":"Way","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2172-5126","authenticated-orcid":false,"given":"Andrew Y","family":"Koh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8511-2041","authenticated-orcid":false,"given":"Sarah","family":"Gaffen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria T","family":"Abreu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7563-484X","authenticated-orcid":false,"given":"Richard J","family":"Bennett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2024.08.16.608271","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T06:53:14Z","timestamp":1768459994000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2024.08.16.608271"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,19]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1101\/2024.08.16.608271","relation":{},"subject":[],"published":{"date-parts":[[2024,8,19]]},"subtype":"preprint"}}