{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T14:31:25Z","timestamp":1780324285715,"version":"3.54.1"},"posted":{"date-parts":[[2024,8,19]]},"group-title":"Bioengineering","reference-count":135,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T00:00:00Z","timestamp":1724025600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,8,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>This study investigates bioelectric stimulation\u2019s role in tissue regeneration by enhancing the piezoelectric properties of tissue-engineered grafts using annealed poly(vinylidene fluoride-trifluoroethylene) (PVDF-TrFE) scaffolds. Annealing at temperatures of 80\u00b0C, 100\u00b0C, 120\u00b0C, and 140\u00b0C was assessed for its impact on material properties and physiological utility. Analytical techniques such as Differential Scanning Calorimetry (DSC), Fourier-Transform Infrared Spectroscopy (FTIR), and X-ray Diffraction (XRD) revealed increased crystallinity with higher annealing temperatures, peaking in \u03b2-phase content and crystallinity at 140\u00b0C. Scanning Electron Microscopy (SEM) showed that 140\u00b0C annealed scaffolds had enhanced lamellar structures, increased porosity, and maximum piezoelectric response. Mechanical tests indicated that 140\u00b0C annealing improved elastic modulus, tensile strength, and substrate stiffness, aligning these properties with physiological soft tissues. In vitro assessments in Schwann cells demonstrated favorable responses, with increased cell proliferation, contraction, and extracellular matrix attachment. Additionally, genes linked to extracellular matrix production, vascularization, and calcium signaling were upregulated. The foreign body response in C57BL\/6 mice, evaluated through Hematoxylin and Eosin (H&amp;E) and Picrosirius Red staining, showed no differences between scaffold groups, supporting the potential for future functional evaluation of the annealed group in tissue repair.<\/jats:p>","DOI":"10.1101\/2024.08.16.608345","type":"posted-content","created":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T14:15:10Z","timestamp":1724076910000},"source":"Crossref","is-referenced-by-count":5,"title":["Enhanced Piezoelectric Performance of PVDF-TrFE Nanofibers through Annealing for Tissue Engineering Applications"],"prefix":"10.64898","author":[{"given":"Maksym","family":"Krutko","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holly M.","family":"Poling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew E.","family":"Bryan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manju","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akaljot","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hasan A.","family":"Reza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn A.","family":"Wikenheiser-Brokamp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6989-3041","authenticated-orcid":false,"given":"Takanori","family":"Takebe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Helmrath","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greg M.","family":"Harris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leyla","family":"Esfandiari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2024082206300430000_2024.08.16.608345v1.1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.180794"},{"key":"2024082206300430000_2024.08.16.608345v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2017.08.032"},{"key":"2024082206300430000_2024.08.16.608345v1.3","doi-asserted-by":"publisher","DOI":"10.4161\/cc.8.21.9888"},{"key":"2024082206300430000_2024.08.16.608345v1.4","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.846917"},{"key":"2024082206300430000_2024.08.16.608345v1.5","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2024.1368630"},{"key":"2024082206300430000_2024.08.16.608345v1.6","doi-asserted-by":"crossref","unstructured":"H. M. Poling et al., \u201cPromoting Human Intestinal Organoid Formation and Stimulation Using Piezoelectric Nanofiber Matrices,\u201d Cold Spring Harbor Laboratory, 2024.","DOI":"10.2139\/ssrn.4871265"},{"key":"2024082206300430000_2024.08.16.608345v1.7","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2020.608442"},{"key":"2024082206300430000_2024.08.16.608345v1.8","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a020487"},{"key":"2024082206300430000_2024.08.16.608345v1.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2022.11.2339"},{"key":"2024082206300430000_2024.08.16.608345v1.10","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a041236"},{"key":"2024082206300430000_2024.08.16.608345v1.11","doi-asserted-by":"publisher","DOI":"10.3390\/genes7090055"},{"key":"2024082206300430000_2024.08.16.608345v1.12","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23031464"},{"key":"2024082206300430000_2024.08.16.608345v1.13","doi-asserted-by":"publisher","DOI":"10.2174\/1574888x15666200129154747"},{"key":"2024082206300430000_2024.08.16.608345v1.14","doi-asserted-by":"publisher","DOI":"10.3390\/medsci11010011"},{"key":"2024082206300430000_2024.08.16.608345v1.15","doi-asserted-by":"publisher","DOI":"10.1089\/bioe.2020.0027"},{"key":"2024082206300430000_2024.08.16.608345v1.16","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.8b00276"},{"key":"2024082206300430000_2024.08.16.608345v1.17","doi-asserted-by":"publisher","DOI":"10.1016\/j.materresbull.2020.111083"},{"key":"2024082206300430000_2024.08.16.608345v1.18","doi-asserted-by":"publisher","DOI":"10.3390\/biom9090448"},{"key":"2024082206300430000_2024.08.16.608345v1.19","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2023.100740"},{"key":"2024082206300430000_2024.08.16.608345v1.20","doi-asserted-by":"publisher","DOI":"10.1038\/s41536-023-00334-y"},{"key":"2024082206300430000_2024.08.16.608345v1.21","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.1c00232"},{"key":"2024082206300430000_2024.08.16.608345v1.22","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.1c03087"},{"key":"2024082206300430000_2024.08.16.608345v1.23","doi-asserted-by":"publisher","DOI":"10.1016\/j.nanoen.2021.105901"},{"key":"2024082206300430000_2024.08.16.608345v1.24","doi-asserted-by":"publisher","DOI":"10.3390\/nano9070952"},{"key":"2024082206300430000_2024.08.16.608345v1.25","doi-asserted-by":"publisher","DOI":"10.1089\/ten.TEB.2015.0279"},{"key":"2024082206300430000_2024.08.16.608345v1.26","doi-asserted-by":"publisher","DOI":"10.1016\/j.nanoen.2022.107707"},{"key":"2024082206300430000_2024.08.16.608345v1.27","doi-asserted-by":"publisher","DOI":"10.1515\/bmt-2021-0265"},{"key":"2024082206300430000_2024.08.16.608345v1.28","doi-asserted-by":"publisher","DOI":"10.3390\/biomimetics9010002"},{"key":"2024082206300430000_2024.08.16.608345v1.29","doi-asserted-by":"publisher","DOI":"10.1093\/milmed\/usad018"},{"key":"2024082206300430000_2024.08.16.608345v1.30","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.202000197"},{"key":"2024082206300430000_2024.08.16.608345v1.31","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioadv.2022.213081"},{"key":"2024082206300430000_2024.08.16.608345v1.32","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2022.151277"},{"key":"2024082206300430000_2024.08.16.608345v1.33","doi-asserted-by":"publisher","DOI":"10.1089\/107632701753337645"},{"issue":"1","key":"2024082206300430000_2024.08.16.608345v1.34","first-page":"3","article-title":"Tissue engineered scaffolds in regenerative medicine,\u201d (in eng)","volume":"3","year":"2014","journal-title":"World J Plast Surg"},{"key":"2024082206300430000_2024.08.16.608345v1.35","doi-asserted-by":"publisher","DOI":"10.1002\/term.1978"},{"key":"2024082206300430000_2024.08.16.608345v1.36","doi-asserted-by":"crossref","unstructured":"L. P. P S, B. Swain , S. Rajput , S. Behera , and S. Parida , \u201cAdvances in P(VDF-TrFE) Composites: A Methodical Review on Enhanced Properties and Emerging Electronics Applications,\u201d Condensed Matter, vol. 8, no. 4, p. 105, 2023. [Online]. Available: https:\/\/www.mdpi.com\/2410-3896\/8\/4\/105.","DOI":"10.3390\/condmat8040105"},{"key":"2024082206300430000_2024.08.16.608345v1.37","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.3c17037"},{"key":"2024082206300430000_2024.08.16.608345v1.38","doi-asserted-by":"publisher","DOI":"10.3390\/ma16134610"},{"key":"2024082206300430000_2024.08.16.608345v1.39","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceramint.2021.07.107"},{"key":"2024082206300430000_2024.08.16.608345v1.40","doi-asserted-by":"publisher","DOI":"10.1063\/1.5023814"},{"key":"2024082206300430000_2024.08.16.608345v1.41","doi-asserted-by":"publisher","DOI":"10.1088\/0957-4484\/17\/10\/034"},{"issue":"41","key":"2024082206300430000_2024.08.16.608345v1.42","doi-asserted-by":"crossref","first-page":"32604","DOI":"10.1039\/C5RA05530J","article-title":"Annealing regulates the performance of an electrospun poly (\u03b5-caprolactone) membrane to accommodate tissue engineering","volume":"5","year":"2015","journal-title":"RSC Advances"},{"key":"2024082206300430000_2024.08.16.608345v1.43","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.2c02863"},{"key":"2024082206300430000_2024.08.16.608345v1.44","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/435938"},{"key":"2024082206300430000_2024.08.16.608345v1.45","doi-asserted-by":"crossref","unstructured":"Y. Ting , Suprapto, N. Bunekar , K. Sivasankar , and Y. R. Aldori , \u201cUsing Annealing Treatment on Fabrication Ionic Liquid-Based PVDF Films,\u201d Coatings, vol. 10, no. 1, p. 44, 2020. [Online]. Available: https:\/\/www.mdpi.com\/2079-6412\/10\/1\/44.","DOI":"10.3390\/coatings10010044"},{"key":"2024082206300430000_2024.08.16.608345v1.46","doi-asserted-by":"publisher","DOI":"10.1002\/app.31631"},{"key":"2024082206300430000_2024.08.16.608345v1.47","doi-asserted-by":"publisher","DOI":"10.1016\/j.matdes.2020.109415"},{"key":"2024082206300430000_2024.08.16.608345v1.48","doi-asserted-by":"publisher","DOI":"10.1021\/acs.macromol.8b02382"},{"key":"2024082206300430000_2024.08.16.608345v1.49","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymer.2019.02.064"},{"key":"2024082206300430000_2024.08.16.608345v1.50","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtla.2019.100461"},{"key":"2024082206300430000_2024.08.16.608345v1.51","doi-asserted-by":"publisher","DOI":"10.1080\/10584587.2012.694748"},{"key":"2024082206300430000_2024.08.16.608345v1.52","doi-asserted-by":"publisher","DOI":"10.3390\/ijms231810365"},{"key":"2024082206300430000_2024.08.16.608345v1.53","doi-asserted-by":"crossref","unstructured":"N. Shehata et al., \u201cStatic-Aligned Piezoelectric Poly (Vinylidene Fluoride) Electrospun Nanofibers\/MWCNT Composite Membrane: Facile Method,\u201d Polymers, vol. 10, no. 9, p. 965, 2018. [Online]. Available: https:\/\/www.mdpi.com\/2073-4360\/10\/9\/965.","DOI":"10.3390\/polym10090965"},{"key":"2024082206300430000_2024.08.16.608345v1.54","doi-asserted-by":"publisher","DOI":"10.3390\/polym10030228"},{"key":"2024082206300430000_2024.08.16.608345v1.55","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9418(03)00003-5"},{"key":"2024082206300430000_2024.08.16.608345v1.56","doi-asserted-by":"publisher","DOI":"10.3390\/nano12040588"},{"key":"2024082206300430000_2024.08.16.608345v1.57","doi-asserted-by":"publisher","DOI":"10.1021\/acsaem.8b00478"},{"key":"2024082206300430000_2024.08.16.608345v1.58","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.10.012"},{"key":"2024082206300430000_2024.08.16.608345v1.59","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(00)00061-3"},{"key":"2024082206300430000_2024.08.16.608345v1.60","doi-asserted-by":"publisher","DOI":"10.5213\/inj.1632600.318"},{"key":"2024082206300430000_2024.08.16.608345v1.61","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2021.09.025"},{"key":"2024082206300430000_2024.08.16.608345v1.62","doi-asserted-by":"publisher","DOI":"10.1177\/2041731414557112"},{"key":"2024082206300430000_2024.08.16.608345v1.63","doi-asserted-by":"publisher","DOI":"10.1097\/IJG.0000000000000108"},{"key":"2024082206300430000_2024.08.16.608345v1.64","doi-asserted-by":"publisher","DOI":"10.1080\/19491034.2020.1742066"},{"key":"2024082206300430000_2024.08.16.608345v1.65","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-012-0928-2"},{"key":"2024082206300430000_2024.08.16.608345v1.66","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-022-04320-w"},{"key":"2024082206300430000_2024.08.16.608345v1.67","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.00023"},{"key":"2024082206300430000_2024.08.16.608345v1.68","doi-asserted-by":"publisher","DOI":"10.1023\/A:1025034925152"},{"key":"2024082206300430000_2024.08.16.608345v1.69","doi-asserted-by":"publisher","DOI":"10.1089\/wound.2013.0517"},{"key":"2024082206300430000_2024.08.16.608345v1.70","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-021-02752-2"},{"key":"2024082206300430000_2024.08.16.608345v1.71","doi-asserted-by":"publisher","DOI":"10.1007\/s00429-020-02036-3"},{"key":"2024082206300430000_2024.08.16.608345v1.72","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22094821"},{"key":"2024082206300430000_2024.08.16.608345v1.73","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2016.03.004"},{"key":"2024082206300430000_2024.08.16.608345v1.74","doi-asserted-by":"publisher","DOI":"10.1002\/wsbm.1479"},{"key":"2024082206300430000_2024.08.16.608345v1.75","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2009.03.002"},{"key":"2024082206300430000_2024.08.16.608345v1.76","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2020.00148"},{"key":"2024082206300430000_2024.08.16.608345v1.77","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.90189"},{"key":"2024082206300430000_2024.08.16.608345v1.78","doi-asserted-by":"crossref","unstructured":"N. Oyama and J. Merregaert , \u201cThe Extracellular Matrix Protein 1 (ECM1) in Molecular-Based Skin Biology,\u201d in Textbook of Aging Skin, M. A. Farage , K. W. Miller , and H. I. Maibach Eds. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014, pp. 1\u201320.","DOI":"10.1007\/978-3-642-27814-3_8-2"},{"key":"2024082206300430000_2024.08.16.608345v1.79","doi-asserted-by":"publisher","DOI":"10.1101\/2022.03.16.22272464"},{"key":"2024082206300430000_2024.08.16.608345v1.80","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2018.01.005"},{"key":"2024082206300430000_2024.08.16.608345v1.81","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2017.09.008"},{"key":"2024082206300430000_2024.08.16.608345v1.82","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20767"},{"key":"2024082206300430000_2024.08.16.608345v1.83","doi-asserted-by":"publisher","DOI":"10.1515\/ntrev-2022-0082"},{"key":"2024082206300430000_2024.08.16.608345v1.84","doi-asserted-by":"publisher","DOI":"10.1039\/d0ra05478j"},{"key":"2024082206300430000_2024.08.16.608345v1.85","doi-asserted-by":"publisher","DOI":"10.3389\/fenrg.2021.621540"},{"key":"2024082206300430000_2024.08.16.608345v1.86","doi-asserted-by":"publisher","DOI":"10.1016\/j.matlet.2018.11.156"},{"key":"2024082206300430000_2024.08.16.608345v1.87","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202102087"},{"key":"2024082206300430000_2024.08.16.608345v1.88","doi-asserted-by":"publisher","DOI":"10.1063\/1.5108867"},{"key":"2024082206300430000_2024.08.16.608345v1.89","doi-asserted-by":"publisher","DOI":"10.3390\/polym9080383"},{"key":"2024082206300430000_2024.08.16.608345v1.90","doi-asserted-by":"publisher","DOI":"10.1016\/j.memsci.2021.120089"},{"key":"2024082206300430000_2024.08.16.608345v1.91","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20662-7"},{"key":"2024082206300430000_2024.08.16.608345v1.92","doi-asserted-by":"crossref","unstructured":"A. Johnston and A. Callanan , \u201cRecent Methods for Modifying Mechanical Properties of Tissue- Engineered Scaffolds for Clinical Applications,\u201d Biomimetics, vol. 8, no. 2, p. 205, 2023. [Online]. Available: https:\/\/www.mdpi.com\/2313-7673\/8\/2\/205.","DOI":"10.3390\/biomimetics8020205"},{"key":"2024082206300430000_2024.08.16.608345v1.93","doi-asserted-by":"publisher","DOI":"10.3390\/nano13243170"},{"key":"2024082206300430000_2024.08.16.608345v1.94","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-3057(03)00062-4"},{"key":"2024082206300430000_2024.08.16.608345v1.95","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymer.2017.08.053"},{"key":"2024082206300430000_2024.08.16.608345v1.96","doi-asserted-by":"publisher","DOI":"10.1088\/2053-1591\/ab4037"},{"key":"2024082206300430000_2024.08.16.608345v1.97","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2022.11.2334"},{"key":"2024082206300430000_2024.08.16.608345v1.98","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultras.2008.02.004"},{"key":"2024082206300430000_2024.08.16.608345v1.99","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201605048"},{"key":"2024082206300430000_2024.08.16.608345v1.100","doi-asserted-by":"publisher","DOI":"10.1098\/rsif.2014.0247"},{"key":"2024082206300430000_2024.08.16.608345v1.101","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2021.110777"},{"key":"2024082206300430000_2024.08.16.608345v1.102","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.1c02457"},{"key":"2024082206300430000_2024.08.16.608345v1.103","doi-asserted-by":"publisher","DOI":"10.1089\/wound.2013.0442"},{"key":"2024082206300430000_2024.08.16.608345v1.104","doi-asserted-by":"publisher","DOI":"10.1089\/wound.2019.1114"},{"key":"2024082206300430000_2024.08.16.608345v1.105","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1982.242.3.R358"},{"key":"2024082206300430000_2024.08.16.608345v1.106","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2024.1386725"},{"key":"2024082206300430000_2024.08.16.608345v1.107","doi-asserted-by":"publisher","DOI":"10.26599\/bsa.2023.9050002"},{"key":"2024082206300430000_2024.08.16.608345v1.108","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.06.006"},{"key":"2024082206300430000_2024.08.16.608345v1.109","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2024.100950"},{"key":"2024082206300430000_2024.08.16.608345v1.110","doi-asserted-by":"publisher","DOI":"10.1186\/1423-0127-16-108"},{"key":"2024082206300430000_2024.08.16.608345v1.111","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2021.617141"},{"key":"2024082206300430000_2024.08.16.608345v1.112","doi-asserted-by":"publisher","DOI":"10.4161\/21541248.2014.992262"},{"key":"2024082206300430000_2024.08.16.608345v1.113","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2021.121269"},{"key":"2024082206300430000_2024.08.16.608345v1.114","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2022.926222"},{"key":"2024082206300430000_2024.08.16.608345v1.115","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2024.01.004"},{"key":"2024082206300430000_2024.08.16.608345v1.116","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2022.01.007"},{"key":"2024082206300430000_2024.08.16.608345v1.117","doi-asserted-by":"publisher","DOI":"10.3390\/cells10113242"},{"key":"2024082206300430000_2024.08.16.608345v1.118","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2022.865266"},{"key":"2024082206300430000_2024.08.16.608345v1.119","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2019.00033"},{"key":"2024082206300430000_2024.08.16.608345v1.120","doi-asserted-by":"publisher","DOI":"10.1002\/glia.24049"},{"key":"2024082206300430000_2024.08.16.608345v1.121","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2017.07.006"},{"key":"2024082206300430000_2024.08.16.608345v1.122","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20030636"},{"key":"2024082206300430000_2024.08.16.608345v1.123","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-15-3262-7_8"},{"key":"2024082206300430000_2024.08.16.608345v1.124","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2012.03.014"},{"key":"2024082206300430000_2024.08.16.608345v1.125","doi-asserted-by":"publisher","DOI":"10.1002\/btm2.10300"},{"key":"2024082206300430000_2024.08.16.608345v1.126","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-010-4132-3"},{"key":"2024082206300430000_2024.08.16.608345v1.127","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2016.08.011"},{"key":"2024082206300430000_2024.08.16.608345v1.128","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7225(65)90019-4"},{"key":"2024082206300430000_2024.08.16.608345v1.129","doi-asserted-by":"publisher","DOI":"10.3390\/cells11132001"},{"key":"2024082206300430000_2024.08.16.608345v1.130","doi-asserted-by":"publisher","DOI":"10.14806\/ej.17.1.200"},{"key":"2024082206300430000_2024.08.16.608345v1.131","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"2024082206300430000_2024.08.16.608345v1.132","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv098"},{"key":"2024082206300430000_2024.08.16.608345v1.133","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1189"},{"key":"2024082206300430000_2024.08.16.608345v1.134","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz114"},{"key":"2024082206300430000_2024.08.16.608345v1.135","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2024.08.16.608345","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T06:53:45Z","timestamp":1768460025000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2024.08.16.608345"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,19]]},"references-count":135,"URL":"https:\/\/doi.org\/10.1101\/2024.08.16.608345","relation":{},"subject":[],"published":{"date-parts":[[2024,8,19]]},"subtype":"preprint"}}