{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"medRxiv"}],"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T14:09:35Z","timestamp":1791468575748,"version":"4.3.4"},"posted":{"date-parts":[[2024,8,28]]},"group-title":"Infectious Diseases (except HIV\/AIDS)","reference-count":18,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T00:00:00Z","timestamp":1724803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,8,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Doxycycline post-exposure prophylaxis (doxy-PEP) for sexually transmitted bacterial infections reduces the risk of syphilis and chlamydia, but effectiveness against gonorrhea is variable, likely attributable to varying resistance rates. As doxy-PEP is incorporated into clinical practice, an urgent unanswered question is whether increased doxycycline use will drive tetracycline-class resistance in\n                  <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n                  . Here, we report an updated RT-PCR molecular diagnostic to detect the\n                  <jats:italic>tetM<\/jats:italic>\n                  gene that confers high-level tetracycline resistance in\n                  <jats:italic>N. gonorrhoeae<\/jats:italic>\n                  .\n                <\/jats:p>","DOI":"10.1101\/2024.08.26.24312240","type":"posted-content","created":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T11:35:49Z","timestamp":1724844949000},"source":"Crossref","is-referenced-by-count":1,"title":["An updated molecular diagnostic for surveillance of\n                  <i>tetM<\/i>\n                  in\n                  <i>Neisseria gonorrhoeae<\/i>"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7849-8078","authenticated-orcid":false,"given":"Samantha G.","family":"Palace","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-4983-6378","authenticated-orcid":false,"given":"Jordan A.","family":"Reyes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7305-897X","authenticated-orcid":false,"given":"Eric","family":"Neubauer Vickers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9774-2435","authenticated-orcid":false,"given":"Aishani V.","family":"Aatresh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8099-8258","authenticated-orcid":false,"given":"Wei","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8466-7547","authenticated-orcid":false,"given":"Zamin","family":"Iqbal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5646-1314","authenticated-orcid":false,"given":"Yonatan H.","family":"Grad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2024083005550934000_2024.08.26.24312240v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30725-9"},{"key":"2024083005550934000_2024.08.26.24312240v1.2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2211934"},{"key":"2024083005550934000_2024.08.26.24312240v1.3","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(24)00236-6"},{"key":"2024083005550934000_2024.08.26.24312240v1.4","doi-asserted-by":"publisher","DOI":"10.1097\/olq.0000000000001471"},{"key":"2024083005550934000_2024.08.26.24312240v1.5","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3181fc695a"},{"key":"2024083005550934000_2024.08.26.24312240v1.6","doi-asserted-by":"crossref","unstructured":"Maduna LD , Kock MM , van der Veer B , Radebe O , McIntyre J , van Alphen LB , Peters RPH . 2020. Antimicrobial Resistance of Neisseria gonorrhoeae Isolates from High-Risk Men in Johannesburg, South Africa. Antimicrob Agents Chemother 64.","DOI":"10.1128\/AAC.00906-20"},{"key":"2024083005550934000_2024.08.26.24312240v1.7","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1097\/OLQ.0000000000001769","article-title":"Predominance of High-Level Tetracycline-Resistant Neisseria gonorrhoeae in Kenya: Implications for Global Implementation of Doxycycline Postexposure Prophylaxis for Prevention of Sexually Transmitted Infections","volume":"50","year":"2023","journal-title":"Sex Transm Dis"},{"key":"2024083005550934000_2024.08.26.24312240v1.8","unstructured":"Centers for Disease Control and Prevention. 2024. Gonococcal Isolate Surveillance Project (GISP) Profiles, 2022. https:\/\/www.cdc.gov\/std\/statistics\/gisp-profiles\/default.htm. Accessed 12 August 2024."},{"key":"2024083005550934000_2024.08.26.24312240v1.9","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1016\/S1473-3099(24)00408-0","article-title":"Modelling molecular and culture-based surveillance of tetracycline resistance in Neisseria gonorrhoeae","volume":"24","year":"2024","journal-title":"Lancet Infect Dis"},{"key":"2024083005550934000_2024.08.26.24312240v1.10","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-199320060-00005"},{"key":"2024083005550934000_2024.08.26.24312240v1.11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2008.01244.x"},{"key":"2024083005550934000_2024.08.26.24312240v1.12","doi-asserted-by":"crossref","first-page":"124","DOI":"10.12688\/wellcomeopenres.14826.1","article-title":"Open-access bacterial population genomics: BIGSdb software, the PubMLST.org website and their applications","volume":"3","year":"2018","journal-title":"Wellcome Open Res"},{"key":"2024083005550934000_2024.08.26.24312240v1.13","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2006.060024"},{"key":"2024083005550934000_2024.08.26.24312240v1.14","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.30.5.664"},{"key":"2024083005550934000_2024.08.26.24312240v1.15","doi-asserted-by":"crossref","first-page":"e0264149","DOI":"10.1371\/journal.pone.0264149","article-title":"Characterization of a Neisseria gonorrhoeae Ciprofloxacin panel for an antimicrobial resistant Isolate Bank","volume":"17","year":"2022","journal-title":"PLoS One"},{"key":"2024083005550934000_2024.08.26.24312240v1.16","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(13)70693-5"},{"key":"2024083005550934000_2024.08.26.24312240v1.17","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1093\/infdis\/jiad027","article-title":"Whole-Genome Sequencing to Predict Antimicrobial Susceptibility Profiles in Neisseria gonorrhoeae","volume":"227","year":"2023","journal-title":"J Infect Dis"},{"key":"2024083005550934000_2024.08.26.24312240v1.18","unstructured":"St. Cyr S , Kreisel K , Pham C. 2022. Gonococcal Isolate Surveillance Project (GISP) and Enhanced GISP (eGISP) protocol. Centers for Disease Control and Prevention, National Center for HIV\/AIDS, Viral Hepatitis, STD, and TB Prevention (U.S.), Division of STD Prevention, Surveillance & Data Management Branch and Laboratory Reference & Research Branch, https:\/\/stacks.cdc.gov\/view\/cdc\/125949."}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2024.08.26.24312240","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T20:10:16Z","timestamp":1768507816000},"score":1,"resource":{"primary":{"URL":"http:\/\/medrxiv.org\/lookup\/doi\/10.1101\/2024.08.26.24312240"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,28]]},"references-count":18,"URL":"https:\/\/doi.org\/10.1101\/2024.08.26.24312240","relation":{},"subject":[],"published":{"date-parts":[[2024,8,28]]},"subtype":"preprint"}}