{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T22:25:16Z","timestamp":1768515916588,"version":"3.49.0"},"posted":{"date-parts":[[2024,8,27]]},"group-title":"Genetics","reference-count":86,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T00:00:00Z","timestamp":1724716800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,8,27]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Sperm small RNAs are implicated in intergenerational transmission of paternal environmental effects. Small RNAs generated by cleavage of tRNAs, known as tRNA fragments (tRFs), are an abundant class of RNAs in mature sperm, and can be modulated by environmental conditions. The ribonuclease(s) responsible for the biogenesis of tRFs in the male reproductive tract remains unknown. Angiogenin, a member of the Ribonuclease A superfamily (RNase A), cleaves tRNAs to generate tRFs in response to cellular stress. Four paralogs of Angiogenin, namely\n                  <jats:italic>Rnase9<\/jats:italic>\n                  ,\n                  <jats:italic>Rnase10, Rnase11<\/jats:italic>\n                  , and\n                  <jats:italic>Rnase12<\/jats:italic>\n                  , are specifically expressed in the epididymis\u2014a long, convoluted tubule where sperm mature and acquire fertility and motility. The biological functions of these genes remain largely unknown. Here, by generating mice deleted for all four genes (\n                  <jats:italic>Rnase9-12-\/-<\/jats:italic>\n                  , termed \u201cKO\u201d for Knock Out), we report that these genes regulate fertility and RNA processing. KO mice showed complete male sterility. KO sperm fertilized oocytes\n                  <jats:italic>in vitro<\/jats:italic>\n                  but failed to efficiently fertilize oocytes\n                  <jats:italic>in vivo,<\/jats:italic>\n                  likely due to an inability of sperm to pass through the utero-tubular junction. Intriguingly, there were decreased levels of fragments of tRNAs (tRFs) and rRNAs (rRNA-derived small RNAs or rsRNAs) in the KO epididymis and epididymal luminal fluid, implying that\n                  <jats:italic>Rnase9-12<\/jats:italic>\n                  regulate the biogenesis and\/or stability of tRFs and rsRNAs. Importantly, KO sperm showed a dramatic decrease in the levels of tRFs, demonstrating a role of\n                  <jats:italic>Rnase9-12<\/jats:italic>\n                  in regulating sperm RNA composition. Together, our results reveal an unexpected role of four epididymis-specific non-canonical RNase A family genes in fertility and RNA processing.\n                <\/jats:p>","DOI":"10.1101\/2024.08.26.608813","type":"posted-content","created":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T16:05:14Z","timestamp":1724774714000},"source":"Crossref","is-referenced-by-count":2,"title":["Epididymis-specific RNase A family genes regulate fertility and small RNA processing"],"prefix":"10.64898","author":[{"given":"Joshua F.","family":"Shaffer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alka","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geetika","family":"Kharkwal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edgardo E.","family":"Linares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew D.","family":"Holmes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sol","family":"Katzman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Upasna","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024083002550793000_2024.08.26.608813v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/216816a0"},{"issue":"5","key":"2024083002550793000_2024.08.26.608813v1.2","doi-asserted-by":"crossref","first-page":"R155","DOI":"10.1530\/REP-18-0589","article-title":"The impact of epididymal proteins on sperm function","volume":"158","year":"2019","journal-title":"Reproduction"},{"key":"2024083002550793000_2024.08.26.608813v1.3","doi-asserted-by":"publisher","DOI":"10.4103\/1008-682X.165946"},{"key":"2024083002550793000_2024.08.26.608813v1.4","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmn055"},{"key":"2024083002550793000_2024.08.26.608813v1.5","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12320"},{"key":"2024083002550793000_2024.08.26.608813v1.6","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1401660210"},{"key":"2024083002550793000_2024.08.26.608813v1.7","doi-asserted-by":"publisher","DOI":"10.1016\/S0070-2153(08)60337-3"},{"key":"2024083002550793000_2024.08.26.608813v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.06.023"},{"key":"2024083002550793000_2024.08.26.608813v1.9","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad6780"},{"key":"2024083002550793000_2024.08.26.608813v1.10","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2012.141"},{"key":"2024083002550793000_2024.08.26.608813v1.11","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-021-00652-7"},{"key":"2024083002550793000_2024.08.26.608813v1.12","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.115.132209"},{"issue":"1","key":"2024083002550793000_2024.08.26.608813v1.13","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1038\/s41467-020-15305-w","article-title":"Reproductive tract extracellular vesicles are sufficient to transmit intergenerational stress and program neurodevelopment","volume":"11","year":"2020","journal-title":"Nat Commun"},{"key":"2024083002550793000_2024.08.26.608813v1.14","doi-asserted-by":"crossref","first-page":"31794","DOI":"10.1038\/srep31794","article-title":"Characterisation of mouse epididymosomes reveals a complex profile of microRNAs and a potential mechanism for modification of the sperm epigenome","volume":"6","year":"2016","journal-title":"Sci Rep"},{"key":"2024083002550793000_2024.08.26.608813v1.15","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2018.00032"},{"key":"2024083002550793000_2024.08.26.608813v1.16","doi-asserted-by":"crossref","unstructured":"Rompala, G.R. , C. 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