{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T22:35:21Z","timestamp":1768516521392,"version":"3.49.0"},"posted":{"date-parts":[[2024,8,26]]},"group-title":"Immunology","reference-count":20,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T00:00:00Z","timestamp":1724630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,8,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Several studies report that ashwagandha, a traditional Ayurvedic supplement, has anti-inflammatory properties. Type 2 (T2) asthma is characterized by eosinophilic airway inflammation. We hypothesized that allergen-induced eosinophilic airway inflammation in mice would be reduced following administration of Withaferin A (WFA), the primary active phytochemical in Ashwagandha. C57BL\/6J mice were given 10 total intra-peritoneal injections of 2 mg\/kg WFA or vehicle control, concurrent with 6 total intranasal administrations of 50 \u00b5g house dust mite extract (HDM) or saline control over 2 weeks. We observed that treatment with WFA reduced allergen-induced peribronchial inflammation and airway eosinophil counts compared to mice treated with controls. In addition, we observed that treatment with WFA reduced lung levels of interleukin-25 (IL-25) but increased lung gene expression levels of its co-receptor,\n                  <jats:italic>Il17ra<\/jats:italic>\n                  , in HDM-challenged mice compared to HDM-challenged mice that received the vehicle control. This study pinpoints a potential mechanism by which WFA modulates allergen-induced airway eosinophilia via the IL-25 signaling pathway. Future studies will investigate the effects of WFA administration on lung eosinophilia and IL-25 signaling in the context of chronic allergen-challenge.\n                <\/jats:p>","DOI":"10.1101\/2024.08.26.609636","type":"posted-content","created":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T23:25:12Z","timestamp":1724887512000},"source":"Crossref","is-referenced-by-count":0,"title":["Withaferin A reduces pulmonary eosinophilia and IL-25 production in a mouse model of allergic airways disease"],"prefix":"10.64898","author":[{"given":"Kevin","family":"Agner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoria L.","family":"McQuade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jack","family":"Womble","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marissa 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