{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T22:06:27Z","timestamp":1771625187793,"version":"3.50.1"},"posted":{"date-parts":[[2024,8,28]]},"group-title":"Neuroscience","reference-count":60,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T00:00:00Z","timestamp":1724803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,4,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>Behavioral timescale synaptic plasticity (BTSP) is a form of synaptic potentiation where a single plateau potential in hippocampal neurons forms a place field during spatial learning. We asked whether BTSP can also form non-spatial responses in the hippocampus and what roles the medial and lateral entorhinal cortex (MEC and LEC) play in driving non-spatial BTSP. Two-photon calcium imaging of dorsal CA1 neurons while mice performed an odor-cued working memory task revealed plateau-like events which formed stable odor-specific responses. These BTSP-like events were much more frequent during the first day of task learning, suggesting that BTSP may be important for early learning. Strong single-neuron stimulation through holographic optogenetics induced plateau-like events and subsequent odor-fields, causally linking BTSP with non-spatial representations. MEC chemogenetic inhibition reduced the frequency of plateau-like events, whereas LEC inhibition reduced potentiation and field-induction probability. Calcium imaging of LEC and MEC temporammonic CA1 projections revealed that MEC axons were more strongly activated by odor presentations, while LEC axons were more odor-selective, further confirming the role of MEC in driving plateau-like events and LEC in relaying odor-specific information. Altogether, odor-specific information from LEC and strong odor-timed activity from MEC are crucial for driving BTSP in CA1, which is a synaptic plasticity mechanism for generation of both spatial and non-spatial responses in the hippocampus.<\/jats:p>","DOI":"10.1101\/2024.08.27.609983","type":"posted-content","created":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T18:25:18Z","timestamp":1724869518000},"source":"Crossref","is-referenced-by-count":5,"title":["Behavioral timescale synaptic plasticity in the hippocampus creates non-spatial representations during learning and is modulated by entorhinal inputs"],"prefix":"10.64898","author":[{"given":"Conor C.","family":"Dorian","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiannis","family":"Taxidis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmet","family":"Arac","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peyman","family":"Golshani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025040307001446000_2024.08.27.609983v2.1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aab3050"},{"key":"2025040307001446000_2024.08.27.609983v2.2","doi-asserted-by":"publisher","DOI":"10.1145\/3561048"},{"key":"2025040307001446000_2024.08.27.609983v2.3","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4062"},{"key":"2025040307001446000_2024.08.27.609983v2.4","doi-asserted-by":"publisher","DOI":"10.1126\/science.aan3846"},{"key":"2025040307001446000_2024.08.27.609983v2.5","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4486"},{"key":"2025040307001446000_2024.08.27.609983v2.6","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-020-0646-2"},{"key":"2025040307001446000_2024.08.27.609983v2.7","doi-asserted-by":"publisher","DOI":"10.1146\/ANNUREV-NEURO-090919-022842"},{"key":"2025040307001446000_2024.08.27.609983v2.8","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.73046"},{"key":"2025040307001446000_2024.08.27.609983v2.9","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05378-6"},{"key":"2025040307001446000_2024.08.27.609983v2.10","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adi3088"},{"key":"2025040307001446000_2024.08.27.609983v2.11","doi-asserted-by":"crossref","unstructured":"K. C. Gonzalez , A. Negrean , Z. Liao , S. Terada , G. Zhang , S. Lee , K. \u00d3csai , B. J. R\u00f3zsa , M. Z. Lin , F. Polleux , et al., Synaptic basis of feature selectivity in hippocampal neurons, Nature (2024) 1\u20139.","DOI":"10.1038\/s41586-024-08325-9"},{"key":"2025040307001446000_2024.08.27.609983v2.12","doi-asserted-by":"crossref","unstructured":"A. D. Madar , A. Jiang , C. Dong , M. E. Sheffield , Synaptic plasticity rules driving representational shifting in the hippocampus, Nature Neuroscience (2025) 1\u201313.","DOI":"10.1038\/s41593-025-01894-6"},{"key":"2025040307001446000_2024.08.27.609983v2.13","doi-asserted-by":"crossref","unstructured":"J. O\u2019Keefe , J. Dostrovsky , The hippocampus as a spatial map: preliminary evidence from unit activity in the freely-moving rat., Brain research (1971).","DOI":"10.1016\/0006-8993(71)90358-1"},{"key":"2025040307001446000_2024.08.27.609983v2.14","doi-asserted-by":"publisher","DOI":"10.1038\/17605"},{"key":"2025040307001446000_2024.08.27.609983v2.15","doi-asserted-by":"publisher","DOI":"10.1016\/j.tics.2018.07.006"},{"key":"2025040307001446000_2024.08.27.609983v2.16","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4653"},{"key":"2025040307001446000_2024.08.27.609983v2.17","doi-asserted-by":"publisher","DOI":"10.1038\/45154"},{"key":"2025040307001446000_2024.08.27.609983v2.18","doi-asserted-by":"publisher","DOI":"10.1037\/0033-295X.99.2.195"},{"key":"2025040307001446000_2024.08.27.609983v2.19","doi-asserted-by":"publisher","DOI":"10.1038\/nature21692"},{"key":"2025040307001446000_2024.08.27.609983v2.20","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2020.08.028"},{"key":"2025040307001446000_2024.08.27.609983v2.21","doi-asserted-by":"publisher","DOI":"10.1126\/science.1159775"},{"key":"2025040307001446000_2024.08.27.609983v2.22","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2011.07.012"},{"key":"2025040307001446000_2024.08.27.609983v2.23","doi-asserted-by":"publisher","DOI":"10.1038\/nrn3827"},{"key":"2025040307001446000_2024.08.27.609983v2.24","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1973.sp010273"},{"key":"2025040307001446000_2024.08.27.609983v2.25","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.31.060407.125639"},{"key":"2025040307001446000_2024.08.27.609983v2.26","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a005751"},{"key":"2025040307001446000_2024.08.27.609983v2.27","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.08.001"},{"key":"2025040307001446000_2024.08.27.609983v2.28","first-page":"69","article-title":"Representation of non-spatial and spatial information in the lateral entorhinal cortex","volume":"5","year":"2011","journal-title":"Frontiers in behavioral neuroscience"},{"key":"2025040307001446000_2024.08.27.609983v2.29","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2013.0369"},{"key":"2025040307001446000_2024.08.27.609983v2.30","doi-asserted-by":"crossref","unstructured":"P. Andersen , R. Morris , D. Amaral , T. Bliss , J. O\u2019Keefe , The hippocampus book, Oxford university press, 2007.","DOI":"10.1093\/acprof:oso\/9780195100273.001.0001"},{"key":"2025040307001446000_2024.08.27.609983v2.31","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2614"},{"key":"2025040307001446000_2024.08.27.609983v2.32","doi-asserted-by":"publisher","DOI":"10.1038\/nature13162"},{"key":"2025040307001446000_2024.08.27.609983v2.33","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4517"},{"key":"2025040307001446000_2024.08.27.609983v2.34","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2020.04.002"},{"key":"2025040307001446000_2024.08.27.609983v2.35","doi-asserted-by":"publisher","DOI":"10.3389\/fncir.2024.1437575"},{"key":"2025040307001446000_2024.08.27.609983v2.36","doi-asserted-by":"publisher","DOI":"10.1126\/science.1099901"},{"key":"2025040307001446000_2024.08.27.609983v2.37","doi-asserted-by":"publisher","DOI":"10.1038\/nature03721"},{"key":"2025040307001446000_2024.08.27.609983v2.38","doi-asserted-by":"publisher","DOI":"10.1126\/science.1125572"},{"issue":"19","key":"2025040307001446000_2024.08.27.609983v2.39","doi-asserted-by":"crossref","first-page":"3135","DOI":"10.1016\/j.neuron.2021.09.019","article-title":"The hippocampus converts dynamic entorhinal inputs into stable spatial maps","volume":"109","year":"2021","journal-title":"Neuron"},{"key":"2025040307001446000_2024.08.27.609983v2.40","doi-asserted-by":"publisher","DOI":"10.1126\/science.1256573"},{"key":"2025040307001446000_2024.08.27.609983v2.41","doi-asserted-by":"publisher","DOI":"10.1101\/061507"},{"key":"2025040307001446000_2024.08.27.609983v2.42","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-019-0502-4"},{"key":"2025040307001446000_2024.08.27.609983v2.43","doi-asserted-by":"publisher","DOI":"10.1126\/science.aav7893"},{"issue":"7959","key":"2025040307001446000_2024.08.27.609983v2.44","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1038\/s41586-023-05928-6","article-title":"Dynamic synchronization between hippocampal representations and stepping","volume":"617","year":"2023","journal-title":"Nature"},{"key":"2025040307001446000_2024.08.27.609983v2.45","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.03.007"},{"key":"2025040307001446000_2024.08.27.609983v2.46","doi-asserted-by":"publisher","DOI":"10.1126\/science.aav5282"},{"key":"2025040307001446000_2024.08.27.609983v2.47","doi-asserted-by":"publisher","DOI":"10.1126\/science.1149381"},{"key":"2025040307001446000_2024.08.27.609983v2.48","doi-asserted-by":"publisher","DOI":"10.1038\/nature12983"},{"key":"2025040307001446000_2024.08.27.609983v2.49","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2015.08.025"},{"key":"2025040307001446000_2024.08.27.609983v2.50","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa5694"},{"key":"2025040307001446000_2024.08.27.609983v2.51","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3236"},{"key":"2025040307001446000_2024.08.27.609983v2.52","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2358-09.2009"},{"key":"2025040307001446000_2024.08.27.609983v2.53","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4418"},{"key":"2025040307001446000_2024.08.27.609983v2.54","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0087-16.2016"},{"key":"2025040307001446000_2024.08.27.609983v2.55","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaj1497"},{"key":"2025040307001446000_2024.08.27.609983v2.56","doi-asserted-by":"publisher","DOI":"10.1126\/science.abm1670"},{"issue":"1","key":"2025040307001446000_2024.08.27.609983v2.57","doi-asserted-by":"crossref","first-page":"6295","DOI":"10.1038\/s41467-024-50546-z","article-title":"Functional architecture of intracellular oscillations in hippocampal dendrites","volume":"15","year":"2024","journal-title":"Nature Communications"},{"key":"2025040307001446000_2024.08.27.609983v2.58","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-08021-8"},{"key":"2025040307001446000_2024.08.27.609983v2.59","doi-asserted-by":"crossref","unstructured":"S. D. Glasgow , T. A. Fisher , E. W. Wong , K. Lancon , K. M. Feighan , I. V. Beamish , J. Gibon , P. S\u00e9gu\u00e9la , E. S. Ruthazer , T. E. Kennedy , Acetylcholine synergizes with netrin-1 to drive persistent firing in the entorhinal cortex, Cell Reports 43 (2) (2024).","DOI":"10.1016\/j.celrep.2024.113812"},{"key":"2025040307001446000_2024.08.27.609983v2.60","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2021.12.003"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2024.08.27.609983","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T06:57:23Z","timestamp":1768460243000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2024.08.27.609983"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,28]]},"references-count":60,"URL":"https:\/\/doi.org\/10.1101\/2024.08.27.609983","relation":{},"subject":[],"published":{"date-parts":[[2024,8,28]]},"subtype":"preprint"}}