{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T22:26:49Z","timestamp":1768516009524,"version":"3.49.0"},"posted":{"date-parts":[[2024,8,30]]},"group-title":"Neuroscience","reference-count":74,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T00:00:00Z","timestamp":1724976000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,8,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Dystroglycan is a cell adhesion molecule that localizes to synapses throughout the nervous system. While Dystroglycan is required to maintain inhibitory synapses from cerebellar molecular layer interneurons (MLIs) onto Purkinje cells (PCs) whether initial synaptogenesis during development is dependent on Dystroglycan has not been examined. We show that conditional deletion of\n                  <jats:italic>Dystroglycan<\/jats:italic>\n                  from Purkinje cells prior to synaptogenesis results in impaired MLI:PC synapse formation and function due to reduced presynaptic inputs and abnormal postsynaptic GABA\n                  <jats:sub>A<\/jats:sub>\n                  receptor clustering. Using genetic manipulations that disrupt glycosylation of Dystroglycan or truncate its cytoplasmic domain, we show that Dystroglycan\u2019s role in synapse function requires both extracellular and intracellular interactions, whereas synapse formation requires only extracellular interactions. Together, these findings provide molecular insight into the mechanism of inhibitory synapse formation and maintenance in cerebellar cortex.\n                <\/jats:p>","DOI":"10.1101\/2024.08.29.610348","type":"posted-content","created":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T19:45:12Z","timestamp":1725047112000},"source":"Crossref","is-referenced-by-count":0,"title":["Distinct functional domains of Dystroglycan regulate inhibitory synapse formation and maintenance in cerebellar Purkinje cells"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2319-6109","authenticated-orcid":false,"given":"Jennifer N.","family":"Jahncke","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5623-5015","authenticated-orcid":false,"given":"Eric","family":"Schnell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5094-5270","authenticated-orcid":false,"given":"Kevin M.","family":"Wright","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024090210350562000_2024.08.29.610348v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(03)03018-X"},{"key":"2024090210350562000_2024.08.29.610348v1.2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012119"},{"key":"2024090210350562000_2024.08.29.610348v1.3","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2020.00164"},{"key":"2024090210350562000_2024.08.29.610348v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2146"},{"key":"2024090210350562000_2024.08.29.610348v1.5","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-12-04805.2002"},{"key":"2024090210350562000_2024.08.29.610348v1.6","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0946-17.2017"},{"key":"2024090210350562000_2024.08.29.610348v1.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2018.08.010"},{"key":"2024090210350562000_2024.08.29.610348v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00907-8"},{"key":"2024090210350562000_2024.08.29.610348v1.9","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02819"},{"key":"2024090210350562000_2024.08.29.610348v1.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.06.035"},{"key":"2024090210350562000_2024.08.29.610348v1.11","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1823-16.2016"},{"key":"2024090210350562000_2024.08.29.610348v1.12","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c140048"},{"key":"2024090210350562000_2024.08.29.610348v1.13","doi-asserted-by":"publisher","DOI":"10.1038\/nature12605"},{"key":"2024090210350562000_2024.08.29.610348v1.14","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4823-05.2006"},{"key":"2024090210350562000_2024.08.29.610348v1.15","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(03)00099-6"},{"key":"2024090210350562000_2024.08.29.610348v1.16","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(00)01195-9"},{"key":"2024090210350562000_2024.08.29.610348v1.17","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22572"},{"key":"2024090210350562000_2024.08.29.610348v1.18","doi-asserted-by":"publisher","DOI":"10.1038\/355696a0"},{"key":"2024090210350562000_2024.08.29.610348v1.19","doi-asserted-by":"publisher","DOI":"10.1126\/science.1214115"},{"key":"2024090210350562000_2024.08.29.610348v1.20","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.87965"},{"key":"2024090210350562000_2024.08.29.610348v1.21","doi-asserted-by":"publisher","DOI":"10.1523\/ENEURO.0149-24.2024"},{"key":"2024090210350562000_2024.08.29.610348v1.22","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.516"},{"key":"2024090210350562000_2024.08.29.610348v1.23","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.45.27305"},{"key":"2024090210350562000_2024.08.29.610348v1.24","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.1999.00887.x"},{"key":"2024090210350562000_2024.08.29.610348v1.25","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adf7084"},{"key":"2024090210350562000_2024.08.29.610348v1.26","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03220-z"},{"key":"2024090210350562000_2024.08.29.610348v1.27","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2011.05.002"},{"key":"2024090210350562000_2024.08.29.610348v1.28","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.2007.04816.x"},{"key":"2024090210350562000_2024.08.29.610348v1.29","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2024.04.010"},{"key":"2024090210350562000_2024.08.29.610348v1.30","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-11-04274.2002"},{"key":"2024090210350562000_2024.08.29.610348v1.31","doi-asserted-by":"publisher","DOI":"10.1038\/mp.2015.31"},{"key":"2024090210350562000_2024.08.29.610348v1.32","doi-asserted-by":"publisher","DOI":"10.1038\/348725a0"},{"key":"2024090210350562000_2024.08.29.610348v1.33","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.42143"},{"key":"2024090210350562000_2024.08.29.610348v1.34","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.70664"},{"key":"2024090210350562000_2024.08.29.610348v1.35","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0307228101"},{"key":"2024090210350562000_2024.08.29.610348v1.36","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3678-13.2014"},{"key":"2024090210350562000_2024.08.29.610348v1.37","doi-asserted-by":"publisher","DOI":"10.1186\/s13064-021-00153-1"},{"key":"2024090210350562000_2024.08.29.610348v1.38","doi-asserted-by":"publisher","DOI":"10.1038\/nature01755"},{"key":"2024090210350562000_2024.08.29.610348v1.39","doi-asserted-by":"publisher","DOI":"10.1186\/1478-811X-8-3"},{"key":"2024090210350562000_2024.08.29.610348v1.40","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3493-06.2006"},{"key":"2024090210350562000_2024.08.29.610348v1.41","doi-asserted-by":"publisher","DOI":"10.1097\/NEN.0b013e318274a128"},{"key":"2024090210350562000_2024.08.29.610348v1.42","doi-asserted-by":"publisher","DOI":"10.1186\/2051-5960-1-58"},{"key":"2024090210350562000_2024.08.29.610348v1.43","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.41.26032"},{"key":"2024090210350562000_2024.08.29.610348v1.44","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.035931"},{"key":"2024090210350562000_2024.08.29.610348v1.45","doi-asserted-by":"publisher","DOI":"10.3233\/JND-210696"},{"key":"2024090210350562000_2024.08.29.610348v1.46","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.113331"},{"key":"2024090210350562000_2024.08.29.610348v1.47","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0802390105"},{"key":"2024090210350562000_2024.08.29.610348v1.48","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2013.07.016"},{"key":"2024090210350562000_2024.08.29.610348v1.49","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.595413"},{"key":"2024090210350562000_2024.08.29.610348v1.50","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.0883"},{"key":"2024090210350562000_2024.08.29.610348v1.51","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3247-10.2010"},{"key":"2024090210350562000_2024.08.29.610348v1.52","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0474-09.2009"},{"key":"2024090210350562000_2024.08.29.610348v1.53","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.07.028"},{"key":"2024090210350562000_2024.08.29.610348v1.54","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"2024090210350562000_2024.08.29.610348v1.55","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-31205-7"},{"key":"2024090210350562000_2024.08.29.610348v1.56","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2018.09.040"},{"key":"2024090210350562000_2024.08.29.610348v1.57","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200105003"},{"key":"2024090210350562000_2024.08.29.610348v1.58","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2006.04170.x"},{"key":"2024090210350562000_2024.08.29.610348v1.59","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1994.tb18624.x"},{"key":"2024090210350562000_2024.08.29.610348v1.60","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1996.tb01568.x"},{"key":"2024090210350562000_2024.08.29.610348v1.61","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-36872-8"},{"key":"2024090210350562000_2024.08.29.610348v1.62","doi-asserted-by":"publisher","DOI":"10.2144\/00291bm09"},{"key":"2024090210350562000_2024.08.29.610348v1.63","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.50712"},{"key":"2024090210350562000_2024.08.29.610348v1.64","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2006.09.003"},{"key":"2024090210350562000_2024.08.29.610348v1.65","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21713"},{"key":"2024090210350562000_2024.08.29.610348v1.66","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-30977-2"},{"key":"2024090210350562000_2024.08.29.610348v1.67","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.42388"},{"key":"2024090210350562000_2024.08.29.610348v1.68","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.10.009"},{"key":"2024090210350562000_2024.08.29.610348v1.69","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1256-21.2022"},{"key":"2024090210350562000_2024.08.29.610348v1.70","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwv021"},{"key":"2024090210350562000_2024.08.29.610348v1.71","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(01)00092-6"},{"key":"2024090210350562000_2024.08.29.610348v1.72","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.89792"},{"key":"2024090210350562000_2024.08.29.610348v1.73","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2015.07.020"},{"key":"2024090210350562000_2024.08.29.610348v1.74","doi-asserted-by":"publisher","DOI":"10.1002\/gene.20062"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2024.08.29.610348","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T06:57:52Z","timestamp":1768460272000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2024.08.29.610348"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,30]]},"references-count":74,"URL":"https:\/\/doi.org\/10.1101\/2024.08.29.610348","relation":{"is-preprint-of":[{"id-type":"doi","id":"10.1038\/s42003-025-08323-1","asserted-by":"subject"}]},"subject":[],"published":{"date-parts":[[2024,8,30]]},"subtype":"preprint"}}