{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:50:05Z","timestamp":1787010605334,"version":"build-2736575974"},"posted":{"date-parts":[[2024,9,1]]},"group-title":"Neuroscience","reference-count":106,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,12,30]]},"abstract":"<jats:title>Summary<\/jats:title>\n                <jats:p>\n                  Region-specific synapse loss is an early pathological hallmark in Alzheimer\u2019s disease (AD). Emerging data in mice and humans highlight microglia, the brain-resident macrophages, as cellular mediators of synapse loss; however, the upstream modulators of microglia-synapse engulfment remain elusive. Here, we report a distinct subset of astrocytes, which are glial cells essential for maintaining synapse homeostasis, appearing in a region-specific manner with age and amyloidosis at onset of synapse loss. These astrocytes are distinguished by their peri-synaptic processes which are \u2018bulbous\u2019 in morphology, contain accumulated p62-immunoreactive bodies, and have reduced territorial domains, resulting in a decrease of astrocyte-synapse coverage. Using integrated\n                  <jats:italic>in vitro<\/jats:italic>\n                  and\n                  <jats:italic>in vivo<\/jats:italic>\n                  approaches, we show that astrocytes upregulate and secrete phagocytic modulator, milk fat globule-EGF factor 8 (MFG-E8), which is sufficient and necessary for promoting microglia-synapse engulfment in their local milieu. Finally, we show that knocking down\n                  <jats:italic>Mfge8<\/jats:italic>\n                  specifically from astrocytes using a viral CRISPR-saCas9 system prevents microglia-synapse engulfment and ameliorates synapse loss in two independent amyloidosis mouse models of AD. Altogether, our findings highlight astrocyte-microglia crosstalk in determining synapse fate in amyloid models and nominate astrocytic MFGE8 as a potential target to ameliorate synapse loss during the earliest stages of AD.\n                <\/jats:p>","DOI":"10.1101\/2024.08.31.606944","type":"posted-content","created":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T16:10:13Z","timestamp":1725207013000},"source":"Crossref","is-referenced-by-count":14,"title":["Astrocyte-derived MFG-E8 facilitates microglial synapse elimination in Alzheimer\u2019s disease mouse models"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8925-5154","authenticated-orcid":false,"given":"Dimitra","family":"Sokolova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shari","family":"Addington Ghansah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Puletti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana","family":"Georgiades","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0640-2417","authenticated-orcid":false,"given":"Sebastiaan","family":"De Schepper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjing","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0436-1332","authenticated-orcid":false,"given":"Gerard","family":"Crowley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9280-6612","authenticated-orcid":false,"given":"Javier","family":"Rueda-Carrasco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angeliki","family":"Koutsiouroumpa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Muckett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4009-282X","authenticated-orcid":false,"given":"Oliver J.","family":"Freeman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0939-1218","authenticated-orcid":false,"given":"Baljit S.","family":"Khakh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5744-4871","authenticated-orcid":false,"given":"Soyon","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025010123150875000_2024.08.31.606944v2.1","doi-asserted-by":"crossref","unstructured":"van Dyck, C. H. , et al. Synaptic density is associated with cognitive performance in Alzheimer\u2019s disease: A PET imaging study with [11C]UCB-J. Alzheimer\u2019s & Dementia 17, (2021).","DOI":"10.1002\/alz.054213"},{"key":"2025010123150875000_2024.08.31.606944v2.2","doi-asserted-by":"publisher","DOI":"10.1002\/alz.12097"},{"key":"2025010123150875000_2024.08.31.606944v2.3","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410270502"},{"key":"2025010123150875000_2024.08.31.606944v2.4","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-2011-101782"},{"key":"2025010123150875000_2024.08.31.606944v2.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2005.09.012"},{"key":"2025010123150875000_2024.08.31.606944v2.6","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-018-0221-2"},{"key":"2025010123150875000_2024.08.31.606944v2.7","first-page":"712","article-title":"Complement and microglia mediate early synapse loss in Alzheimer mouse models","volume":"352","year":"1979","journal-title":"Science"},{"key":"2025010123150875000_2024.08.31.606944v2.8","first-page":"e113246","article-title":"Microglia-synapse engulfment via PtdSer-TREM2 ameliorates neuronal hyperactivity in Alzheimer\u2019s disease models","volume":"44","year":"2023","journal-title":"EMBO J"},{"key":"2025010123150875000_2024.08.31.606944v2.9","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-023-01257-z"},{"key":"2025010123150875000_2024.08.31.606944v2.10","doi-asserted-by":"publisher","DOI":"10.1038\/s43587-022-00281-1"},{"key":"2025010123150875000_2024.08.31.606944v2.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2019.12.004"},{"key":"2025010123150875000_2024.08.31.606944v2.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.11.013"},{"key":"2025010123150875000_2024.08.31.606944v2.13","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.04.001"},{"key":"2025010123150875000_2024.08.31.606944v2.14","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2023.101175"},{"key":"2025010123150875000_2024.08.31.606944v2.15","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2018.10.014"},{"key":"2025010123150875000_2024.08.31.606944v2.16","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-018-0334-7"},{"key":"2025010123150875000_2024.08.31.606944v2.17","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(98)01349-6"},{"key":"2025010123150875000_2024.08.31.606944v2.18","doi-asserted-by":"crossref","unstructured":"Chung, W. S. , Allen, N. J. & Eroglu, C . Astrocytes control synapse formation, function, and elimination. Cold Spring Harb Perspect Biol 7, (2015).","DOI":"10.1101\/cshperspect.a020370"},{"key":"2025010123150875000_2024.08.31.606944v2.19","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2017.09.056"},{"key":"2025010123150875000_2024.08.31.606944v2.20","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2023.09.006"},{"key":"2025010123150875000_2024.08.31.606944v2.21","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25021160"},{"key":"2025010123150875000_2024.08.31.606944v2.22","doi-asserted-by":"publisher","DOI":"10.1038\/nature12776"},{"key":"2025010123150875000_2024.08.31.606944v2.23","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-03060-3"},{"issue":"1979","key":"2025010123150875000_2024.08.31.606944v2.24","first-page":"1269","article-title":"Astrocyte-derived interleukin-33 promotes microglial synapse engulfment and neural circuit development","volume":"1273","year":"2018","journal-title":"Science"},{"key":"2025010123150875000_2024.08.31.606944v2.25","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3697"},{"key":"2025010123150875000_2024.08.31.606944v2.26","doi-asserted-by":"publisher","DOI":"10.1038\/417182a"},{"key":"2025010123150875000_2024.08.31.606944v2.27","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.12288"},{"key":"2025010123150875000_2024.08.31.606944v2.28","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4837-11.2012"},{"key":"2025010123150875000_2024.08.31.606944v2.29","doi-asserted-by":"crossref","unstructured":"Shah, D. et al. Astrocyte calcium dysfunction causes early network hyperactivity in Alzheimer\u2019s disease. Cell Rep 40, (2022).","DOI":"10.1016\/j.celrep.2022.111280"},{"key":"2025010123150875000_2024.08.31.606944v2.30","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4043"},{"key":"2025010123150875000_2024.08.31.606944v2.31","unstructured":"Escartin, C. et al. Reactive astrocyte nomenclature, definitions, and future directions. Nat Neurosci (2021)."},{"key":"2025010123150875000_2024.08.31.606944v2.32","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-009-0619-8"},{"key":"2025010123150875000_2024.08.31.606944v2.33","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.201210949"},{"key":"2025010123150875000_2024.08.31.606944v2.34","doi-asserted-by":"crossref","unstructured":"Endo, F. et al. Molecular basis of astrocyte diversity and morphology across the CNS in health and disease. Science (1979) 378, (2022).","DOI":"10.1126\/science.adc9020"},{"key":"2025010123150875000_2024.08.31.606944v2.35","doi-asserted-by":"crossref","unstructured":"Zhang, Z. et al. The Appropriate Marker for Astrocytes: Comparing the Distribution and Expression of Three Astrocytic Markers in Different Mouse Cerebral Regions. Biomed Res Int 2019, 1\u201315 (2019).","DOI":"10.1155\/2019\/9605265"},{"key":"2025010123150875000_2024.08.31.606944v2.36","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-022-01409-5"},{"key":"2025010123150875000_2024.08.31.606944v2.37","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2020.101255"},{"key":"2025010123150875000_2024.08.31.606944v2.38","doi-asserted-by":"publisher","DOI":"10.1007\/BF02816112"},{"key":"2025010123150875000_2024.08.31.606944v2.39","first-page":"1443","article-title":"Age-Associated Inclusions in Normal and Transgenic Mouse Brain","volume":"255","year":"1979","journal-title":"Science"},{"key":"2025010123150875000_2024.08.31.606944v2.40","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-22624-z"},{"key":"2025010123150875000_2024.08.31.606944v2.41","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-018-0057-0"},{"key":"2025010123150875000_2024.08.31.606944v2.42","doi-asserted-by":"publisher","DOI":"10.1038\/s43587-022-00257-1"},{"key":"2025010123150875000_2024.08.31.606944v2.43","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.06.038"},{"key":"2025010123150875000_2024.08.31.606944v2.44","doi-asserted-by":"publisher","DOI":"10.1007\/BF00308809"},{"key":"2025010123150875000_2024.08.31.606944v2.45","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.119.230458"},{"key":"2025010123150875000_2024.08.31.606944v2.46","doi-asserted-by":"crossref","unstructured":"Aug\u00e9, E. , Duran, J. , Guinovart, J. J. , Pelegr\u00ed, C. & Vilaplana, J . Exploring the elusive composition of corpora amylacea of human brain. Sci Rep 8, (2018).","DOI":"10.1038\/s41598-018-31766-y"},{"key":"2025010123150875000_2024.08.31.606944v2.47","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2021.101484"},{"key":"2025010123150875000_2024.08.31.606944v2.48","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-022-02412-9"},{"key":"2025010123150875000_2024.08.31.606944v2.49","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2019.05.024"},{"issue":"553","key":"2025010123150875000_2024.08.31.606944v2.50","first-page":"e1","article-title":"Glutamate transporter variants reduce glutamate uptake in Alzheimer\u2019s disease","volume":"32","year":"2011","journal-title":"Neurobiol Aging"},{"key":"2025010123150875000_2024.08.31.606944v2.51","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-199708000-00008"},{"key":"2025010123150875000_2024.08.31.606944v2.52","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-2007-11113"},{"key":"2025010123150875000_2024.08.31.606944v2.53","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2022.10.013"},{"key":"2025010123150875000_2024.08.31.606944v2.54","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4178-07.2008"},{"key":"2025010123150875000_2024.08.31.606944v2.55","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1800165115"},{"key":"2025010123150875000_2024.08.31.606944v2.56","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-14198-8"},{"key":"2025010123150875000_2024.08.31.606944v2.57","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa1934"},{"key":"2025010123150875000_2024.08.31.606944v2.58","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.06.021"},{"key":"2025010123150875000_2024.08.31.606944v2.59","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-018-0656-4"},{"key":"2025010123150875000_2024.08.31.606944v2.60","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05440-3"},{"key":"2025010123150875000_2024.08.31.606944v2.61","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.07.072"},{"key":"2025010123150875000_2024.08.31.606944v2.62","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-023-02476-4"},{"key":"2025010123150875000_2024.08.31.606944v2.63","first-page":"1147","article-title":"Autoimmune disease and impaired uptake of apoptotic cells in MFG-E8-deficient mice","volume":"304","year":"1979","journal-title":"Science"},{"key":"2025010123150875000_2024.08.31.606944v2.64","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20092401"},{"key":"2025010123150875000_2024.08.31.606944v2.65","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.05.012"},{"key":"2025010123150875000_2024.08.31.606944v2.66","doi-asserted-by":"publisher","DOI":"10.1038\/416535a"},{"key":"2025010123150875000_2024.08.31.606944v2.67","doi-asserted-by":"publisher","DOI":"10.1038\/nm1782"},{"key":"2025010123150875000_2024.08.31.606944v2.68","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2023.12.003"},{"key":"2025010123150875000_2024.08.31.606944v2.69","doi-asserted-by":"crossref","unstructured":"Mucke, L. , et al. High-Level Neuronal Expression of A 1-42 in Wild-Type Human Amyloid Protein Precursor Transgenic Mice: Synaptotoxicity without Plaque Formation. (2000).","DOI":"10.1523\/JNEUROSCI.20-11-04050.2000"},{"key":"2025010123150875000_2024.08.31.606944v2.70","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007677003387"},{"key":"2025010123150875000_2024.08.31.606944v2.71","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-020-00783-4"},{"key":"2025010123150875000_2024.08.31.606944v2.72","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2018.03.003"},{"key":"2025010123150875000_2024.08.31.606944v2.73","doi-asserted-by":"crossref","unstructured":"Popov, A. et al. Astrocyte dystrophy in ageing brain parallels impaired synaptic plasticity. Aging Cell 20, (2021).","DOI":"10.1111\/acel.13334"},{"key":"2025010123150875000_2024.08.31.606944v2.74","doi-asserted-by":"crossref","unstructured":"Popov, A. et al. Mitochondrial malfunction and atrophy of astrocytes in the aged human cerebral cortex. Nat Commun 14, (2023).","DOI":"10.1038\/s41467-023-44192-0"},{"key":"2025010123150875000_2024.08.31.606944v2.75","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.07.025"},{"key":"2025010123150875000_2024.08.31.606944v2.76","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhz016"},{"key":"2025010123150875000_2024.08.31.606944v2.77","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1206171109"},{"key":"2025010123150875000_2024.08.31.606944v2.78","first-page":"559","article-title":"A vicious cycle of \u03b2 amyloid\u2013dependent neuronal hyperactivation","volume":"365","year":"1979","journal-title":"Science"},{"key":"2025010123150875000_2024.08.31.606944v2.79","doi-asserted-by":"publisher","DOI":"10.1126\/science.1074069"},{"key":"2025010123150875000_2024.08.31.606944v2.80","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-06502-w"},{"key":"2025010123150875000_2024.08.31.606944v2.81","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2017.188"},{"key":"2025010123150875000_2024.08.31.606944v2.82","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2014.12.032"},{"key":"2025010123150875000_2024.08.31.606944v2.83","doi-asserted-by":"crossref","unstructured":"Lee, H.-G. , Lee, J.-H. , Flausino, L. E. & Quintana, F. J . Neuroinflammation: An astrocyte perspective. Sci Transl Med 15, (2023).","DOI":"10.1126\/scitranslmed.adi7828"},{"key":"2025010123150875000_2024.08.31.606944v2.84","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-015-0152-4"},{"key":"2025010123150875000_2024.08.31.606944v2.85","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-023-01158-0"},{"key":"2025010123150875000_2024.08.31.606944v2.86","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2022.01.017"},{"key":"2025010123150875000_2024.08.31.606944v2.87","doi-asserted-by":"publisher","DOI":"10.1186\/s13041-019-0504-x"},{"key":"2025010123150875000_2024.08.31.606944v2.88","unstructured":"Paolicelli, R. C. , et al. Microglia States and Nomenclature: A Field at Its Crossroads. vol. 34 (2022)."},{"key":"2025010123150875000_2024.08.31.606944v2.89","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25021160"},{"key":"2025010123150875000_2024.08.31.606944v2.90","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.03.026"},{"key":"2025010123150875000_2024.08.31.606944v2.91","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.05.018"},{"key":"2025010123150875000_2024.08.31.606944v2.92","doi-asserted-by":"crossref","unstructured":"Damisah, E. C. et al. Astrocytes and microglia play orchestrated roles and respect phagocytic territories during neuronal corpse removal in vivo. 1\u201313 (2020).","DOI":"10.1126\/sciadv.aba3239"},{"key":"2025010123150875000_2024.08.31.606944v2.93","doi-asserted-by":"crossref","unstructured":"Konishi, H. et al. Astrocytic phagocytosis is a compensatory mechanism for microglial dysfunction. EMBO J 39, (2020).","DOI":"10.15252\/embj.2020104464"},{"key":"2025010123150875000_2024.08.31.606944v2.94","doi-asserted-by":"publisher","DOI":"10.1101\/2020.04.24.059584"},{"key":"2025010123150875000_2024.08.31.606944v2.95","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2021107915"},{"key":"2025010123150875000_2024.08.31.606944v2.96","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2022111790"},{"key":"2025010123150875000_2024.08.31.606944v2.97","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.07.095"},{"key":"2025010123150875000_2024.08.31.606944v2.98","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2019102378"},{"key":"2025010123150875000_2024.08.31.606944v2.99","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-019-0167-y"},{"key":"2025010123150875000_2024.08.31.606944v2.100","doi-asserted-by":"crossref","unstructured":"Sokolova, D. , Childs, T. & Hong, S . Insight into the role of phosphatidylserine in complement-mediated synapse loss in Alzheimer\u2019s disease. Fac Rev 10, (2021).","DOI":"10.12703\/r\/10-19"},{"key":"2025010123150875000_2024.08.31.606944v2.101","doi-asserted-by":"publisher","DOI":"10.1038\/nature21029"},{"key":"2025010123150875000_2024.08.31.606944v2.102","doi-asserted-by":"publisher","DOI":"10.1172\/jci138179"},{"key":"2025010123150875000_2024.08.31.606944v2.103","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3599"},{"key":"2025010123150875000_2024.08.31.606944v2.104","first-page":"1248","article-title":"An environment-dependent transcriptional network specifies human microglia identity","volume":"356","year":"1979","journal-title":"Science"},{"key":"2025010123150875000_2024.08.31.606944v2.105","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2010.06.001"},{"key":"2025010123150875000_2024.08.31.606944v2.106","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2018.09.017"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2024.08.31.606944","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T06:58:28Z","timestamp":1768460308000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2024.08.31.606944"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,1]]},"references-count":106,"URL":"https:\/\/doi.org\/10.1101\/2024.08.31.606944","relation":{},"subject":[],"published":{"date-parts":[[2024,9,1]]},"subtype":"preprint"}}