{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T12:28:27Z","timestamp":1777984107233,"version":"3.51.4"},"posted":{"date-parts":[[2024,9,3]]},"group-title":"Immunology","reference-count":70,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,9,3]],"date-time":"2024-09-03T00:00:00Z","timestamp":1725321600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,4,3]]},"abstract":"<jats:title>Summary<\/jats:title>\n                <jats:p>\n                  Although mechanical ventilation is a critical intervention for acute respiratory distress syndrome (ARDS), it can trigger an IL-1\u03b2-associated complication known as ventilator-induced lung injury. In mice, we found that LPS and high-volume ventilation, LPS-HVV, leads to hypoxemia with neutrophil extracellular traps (NETs) formation in the alveoli. Furthermore,\n                  <jats:italic>\n                    Il1r1\n                    <jats:sup>-\/-<\/jats:sup>\n                  <\/jats:italic>\n                  LPS-HVV mice did not develop hypoxemia and had reduced NETs, indicating that IL-1R1 signaling is important for NETs formation and hypoxemia. Therapeutic hypothermia (TH) is known to reduce the release of inflammatory mediators. In LPS-HVV mice, TH (32 \u00b0C body temperature) prevented hypoxemia development, reducing albumin leakage, IL-1\u03b2, gasdermin D (GSDMD) and NETs formation. We also observed that LPS-primed macrophages, when stimulated at 32\u00b0C with ATP or nigericin, release less IL-1\u03b2 associated with reduced GSDMD cleavage. Thus, hypothermia is an important modulating factor in the NLRP3 inflammasome activation, IL-1\u03b2 release and NETs formation, preventing LPS-HVV-induced acute respiratory failure.\n                <\/jats:p>","DOI":"10.1101\/2024.09.02.610778","type":"posted-content","created":{"date-parts":[[2024,9,3]],"date-time":"2024-09-03T17:55:21Z","timestamp":1725386121000},"source":"Crossref","is-referenced-by-count":0,"title":["Hypothermia protects against ventilator-induced lung injury by limiting IL-1\u03b2 release and NETs formation"],"prefix":"10.64898","author":[{"given":"Nobuyuki","family":"Nosaka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa","family":"Borges","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisy","family":"Martinon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy 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