{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T22:30:36Z","timestamp":1768516236247,"version":"3.49.0"},"posted":{"date-parts":[[2024,9,7]]},"group-title":"Developmental Biology","reference-count":84,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,9,7]],"date-time":"2024-09-07T00:00:00Z","timestamp":1725667200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,9,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  The surface of epithelial tissues is covered by an apical extracellular matrix (aECM). The aECMs of different tissues have distinct compositions to serve distinct functions, yet how a particular cell type assembles the proper aECM is not well understood. We used the cell-type specific matrix of the\n                  <jats:italic>C. elegans<\/jats:italic>\n                  vulva to investigate the connection between cell identity and matrix assembly. The vulva is an epithelial tube composed of seven cell types descending from EGFR\/Ras-dependent (1\u00b0) and Notch-dependent (2\u00b0) lineages. Vulva aECM contains multiple Zona Pellucida domain (ZP) proteins, which are a common component of aECMs across life. ZP proteins LET-653 and CUTL-18 assemble on 1\u00b0 cell surfaces, while NOAH-1 assembles on a subset of 2\u00b0 surfaces. All three ZP genes are broadly transcribed, indicating that cell-type specific ZP assembly must be determined by features of the destination cell surface. The paired box (Pax) transcription factor EGL-38 promotes assembly of 1\u00b0 matrix and prevents inappropriate assembly of 2\u00b0 matrix, suggesting that EGL-38 promotes expression of one or more ZP matrix organizers. Our results connect the known signaling pathways and various downstream effectors to EGL-38\/Pax expression and the ZP matrix component of vulva cell fate execution. We propose that dedicated transcriptional networks may contribute to cell-appropriate assembly of aECM in many epithelial organs.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Highlights<\/jats:title>\n                  <jats:list list-type=\"bullet\">\n                    <jats:list-item>\n                      <jats:p>\n                        <jats:italic>C. elegans<\/jats:italic>\n                        vulva apical extracellular matrix is cell-type specific\n                      <\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>Broadly transcribed Zona Pellucida domain proteins assemble in specific matrices<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>The Pax2\/5\/8 homolog EGL-38 promotes assembly of the 1\u00b0 vulva cell matrix<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>EGL-38 expression and 1\u00b0 cell matrix assembly depend on EGFR signaling<\/jats:p>\n                    <\/jats:list-item>\n                  <\/jats:list>\n                <\/jats:sec>","DOI":"10.1101\/2024.09.04.611291","type":"posted-content","created":{"date-parts":[[2024,9,7]],"date-time":"2024-09-07T04:45:20Z","timestamp":1725684320000},"source":"Crossref","is-referenced-by-count":0,"title":["The Pax transcription factor EGL-38 links EGFR signaling to assembly of a cell-type specific apical extracellular matrix in the\n                  <i>Caenorhabditis elegans<\/i>\n                  vulva"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3449-2790","authenticated-orcid":false,"given":"Helen F.","family":"Schmidt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0928-7434","authenticated-orcid":false,"given":"Chelsea B.","family":"Darwin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2940-8750","authenticated-orcid":false,"given":"Meera V.","family":"Sundaram","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024091006150630000_2024.09.04.611291v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-43058-9"},{"key":"2024091006150630000_2024.09.04.611291v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2016.01.006"},{"key":"2024091006150630000_2024.09.04.611291v1.3","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.37.28866"},{"key":"2024091006150630000_2024.09.04.611291v1.4","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.24.3149"},{"key":"2024091006150630000_2024.09.04.611291v1.5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1010944"},{"key":"2024091006150630000_2024.09.04.611291v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ctdb.2018.02.007"},{"key":"2024091006150630000_2024.09.04.611291v1.7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1519803113"},{"key":"2024091006150630000_2024.09.04.611291v1.8","doi-asserted-by":"publisher","DOI":"10.7554\/ettife.08887"},{"key":"2024091006150630000_2024.09.04.611291v1.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2019.11.015"},{"key":"2024091006150630000_2024.09.04.611291v1.10","doi-asserted-by":"publisher","DOI":"10.1242\/dev.124.20.3919"},{"key":"2024091006150630000_2024.09.04.611291v1.11","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(99)80112-2"},{"key":"2024091006150630000_2024.09.04.611291v1.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2021.01.010"},{"key":"2024091006150630000_2024.09.04.611291v1.13","unstructured":"Clark, D.V. , 1990. 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