{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T22:34:15Z","timestamp":1768516455910,"version":"3.49.0"},"posted":{"date-parts":[[2024,9,11]]},"group-title":"Cell Biology","reference-count":56,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T00:00:00Z","timestamp":1726012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,9,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Microtubule (MT) regulation is essential for oocyte development. In\n                  <jats:italic>Drosophila<\/jats:italic>\n                  , MT stability, polarity, abundance, and orientation undergo dynamic changes across developmental stages. In our effort to identify novel microtubule-associated proteins (MAPs) that regulate MTs in the\n                  <jats:italic>Drosophila<\/jats:italic>\n                  ovary, we identified a previously uncharacterized gene, CG18190, encoding a novel MT end-binding (EB) protein, which we propose to name EB-SUN. We show that EB-SUN colocalizes with EB1 at growing microtubule plus-ends in\n                  <jats:italic>Drosophila<\/jats:italic>\n                  S2 cells. Tissue-specific and developmental expression profiles from Paralog Explorer reveal that EB-SUN is predominantly expressed in the ovary and early embryos, while EB1 is ubiquitously expressed. Furthermore, as early as oocyte determination, EB-SUN comets are highly concentrated in oocytes during oogenesis. EB-SUN knockout (KO) results in a decrease in MT density at the onset of mid-oogenesis (Stage 7) and delays oocyte growth during late mid-oogenesis (Stage 9). Combining EB-SUN KO with EB1 knockdown (KD) in germ cells significantly further reduced MT density at Stage 7. Notably, all eggs from EB-SUN KO\/EB1 KD females fail to hatch, unlike single gene depletion, suggesting a functional redundancy between these two EB proteins during embryogenesis. Our findings indicate that EB-SUN and EB1 play distinct roles during early embryogenesis.\n                <\/jats:p>\n                <jats:sec id=\"s1\">\n                  <jats:title>Significance Statement<\/jats:title>\n                  <jats:list list-type=\"bullet\">\n                    <jats:list-item>\n                      <jats:p>\n                        Our study identified a novel microtubule end-binding (EB) protein, which is highly expressed in the\n                        <jats:italic>Drosophila<\/jats:italic>\n                        ovary, and we propose to name it EB-SUN.\n                      <\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>We demonstrate the functional redundancy of EB-SUN and EB1 during oocyte development, while highlighting their distinct roles in early embryogenesis.<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>\n                        Given the high conservation of MT-dependent mechanisms in oogenesis and EB proteins across species, these findings provide insights into the differential regulation and tissue-specific functions of the EB family, using\n                        <jats:italic>Drosophila<\/jats:italic>\n                        as a model system, potentially benefiting future research on MT regulation in higher organisms.\n                      <\/jats:p>\n                    <\/jats:list-item>\n                  <\/jats:list>\n                <\/jats:sec>","DOI":"10.1101\/2024.09.11.612465","type":"posted-content","created":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T18:50:15Z","timestamp":1726080615000},"source":"Crossref","is-referenced-by-count":0,"title":["EB-SUN, a New Microtubule Plus-End Tracking Protein in\n                  <i>Drosophila<\/i>"],"prefix":"10.64898","author":[{"given":"Sun K.","family":"Kim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen L.","family":"Rogers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brad S.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6361-2798","authenticated-orcid":false,"given":"Vladimir I.","family":"Gelfand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024091522282344000_2024.09.11.612465v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2369"},{"key":"2024091522282344000_2024.09.11.612465v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2005.02.001"},{"key":"2024091522282344000_2024.09.11.612465v1.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2008.09.011"},{"key":"2024091522282344000_2024.09.11.612465v1.4","doi-asserted-by":"publisher","DOI":"10.1242\/dev.118.2.401"},{"key":"2024091522282344000_2024.09.11.612465v1.5","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5487.2120"},{"key":"2024091522282344000_2024.09.11.612465v1.6","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-01803-0"},{"key":"2024091522282344000_2024.09.11.612465v1.7","doi-asserted-by":"publisher","DOI":"10.1038\/ng2049"},{"key":"2024091522282344000_2024.09.11.612465v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/s0074-7696(01)03016-9"},{"key":"2024091522282344000_2024.09.11.612465v1.9","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.068130"},{"key":"2024091522282344000_2024.09.11.612465v1.10","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.10140"},{"key":"2024091522282344000_2024.09.11.612465v1.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.03.007"},{"key":"2024091522282344000_2024.09.11.612465v1.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2021.11.005"},{"key":"2024091522282344000_2024.09.11.612465v1.13","unstructured":"Gelbart, W. , & Emmert, D . 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