{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T04:22:14Z","timestamp":1788236534823,"version":"build-2803163510"},"posted":{"date-parts":[[2024,9,15]]},"group-title":"Neuroscience","reference-count":132,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,9,15]],"date-time":"2024-09-15T00:00:00Z","timestamp":1726358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  \u03b3-aminobutyric acid (GABA) is an abundant neurotransmitter that plays multiple roles in the vertebrate central nervous system (CNS). In the early developing CNS, GABAergic signaling acts to depolarize cells. It mediates several aspects of neural development, including cell proliferation, neuronal migration, neurite growth, and synapse formation, as well as the development of critical periods. Later in CNS development, GABAergic signaling acts in an inhibitory manner when it becomes the predominant inhibitory neurotransmitter in the brain. This behavior switch occurs due to changes in chloride\/cation transporter expression. Abnormalities of GABAergic signaling appear to underlie several human neurological conditions, including seizure disorders. However, the impact of reduced GABAergic signaling on brain development has been challenging to study in mammals. Here we take advantage of zebrafish and light sheet imaging to assess the impact of reduced GABAergic signaling on the functional circuitry in the larval zebrafish optic tectum. Zebrafish have three\n                  <jats:italic>gad<\/jats:italic>\n                  genes: two\n                  <jats:italic>gad1<\/jats:italic>\n                  paralogs known as\n                  <jats:italic>gad1a<\/jats:italic>\n                  and\n                  <jats:italic>gad1b<\/jats:italic>\n                  , and\n                  <jats:italic>gad2.<\/jats:italic>\n                  The\n                  <jats:italic>gad1b<\/jats:italic>\n                  and\n                  <jats:italic>gad2<\/jats:italic>\n                  genes are expressed in the developing optic tectum. Null mutations in\n                  <jats:italic>gad1b<\/jats:italic>\n                  significantly reduce GABA levels in the brain and increase electrophysiological activity in the optic tectum. Fast light sheet imaging of genetically encoded calcium indicator (GCaMP)-expressing\n                  <jats:italic>gab1b<\/jats:italic>\n                  null larval zebrafish revealed patterns of neural activity that were different than either gad1b-normal larvae or\n                  <jats:italic>gad1b<\/jats:italic>\n                  -normal larvae acutely exposed to pentylenetetrazole (PTZ). These results demonstrate that reduced GABAergic signaling during development increases functional connectivity and concomitantly hyper-synchronization of neuronal networks.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Significance Statement<\/jats:title>\n                  <jats:p>\n                    Understanding the impact of reduced GABAergic signaling on vertebrate brain development and function will help elucidate the etiology of seizure initiation and propagation and other neurological disorders due to the altered formation of neural circuits. Here, we used fast light sheet imaging of larval zebrafish that neuronally expressed a genetically encoded calcium indicator (GCaMP) to assess the impact of reduced GABA levels through null mutation of\n                    <jats:italic>gad1b<\/jats:italic>\n                    during brain development. We show that reduced GABA levels during development result in increased functional connectivity in the brain.\n                  <\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2024.09.11.612511","type":"posted-content","created":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T01:38:26Z","timestamp":1726450706000},"source":"Crossref","is-referenced-by-count":4,"title":["Decreased GABA levels during development result in increased connectivity in the larval zebrafish tectum"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8168-5421","authenticated-orcid":false,"given":"Yang","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2800-5949","authenticated-orcid":false,"given":"Yongkai","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2747-6506","authenticated-orcid":false,"given":"Carly R.","family":"Duffy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7725-9534","authenticated-orcid":false,"given":"Ariel J","family":"VanLeuven","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2404-369X","authenticated-orcid":false,"given":"John Branson","family":"Byers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9459-5548","authenticated-orcid":false,"given":"Hannah C.","family":"Schriever","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca E.","family":"Ball","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica M.","family":"Carpenter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0007-7289","authenticated-orcid":false,"given":"Chelsea E.","family":"Gunderson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8747-5065","authenticated-orcid":false,"given":"Nikolay M.","family":"Filipov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7555-5180","authenticated-orcid":false,"given":"Peter A.","family":"Kner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7503-0528","authenticated-orcid":false,"given":"James D.","family":"Lauderdale","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2024091822501989000_2024.09.11.612511v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2346"},{"key":"2024091822501989000_2024.09.11.612511v1.2","first-page":"104","article-title":"Two-photon calcium imaging during fictive navigation in virtual environments","volume":"7","year":"2013","journal-title":"Front Neural Circuits"},{"key":"2024091822501989000_2024.09.11.612511v1.3","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2434"},{"key":"2024091822501989000_2024.09.11.612511v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2007.06.002"},{"key":"2024091822501989000_2024.09.11.612511v1.5","doi-asserted-by":"publisher","DOI":"10.1038\/srep29490"},{"key":"2024091822501989000_2024.09.11.612511v1.6","doi-asserted-by":"publisher","DOI":"10.1038\/nn2030"},{"key":"2024091822501989000_2024.09.11.612511v1.7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.12.6496"},{"key":"2024091822501989000_2024.09.11.612511v1.8","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.1898"},{"key":"2024091822501989000_2024.09.11.612511v1.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2017.06.056"},{"key":"2024091822501989000_2024.09.11.612511v1.10","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1109\/TMI.2020.3036933","article-title":"Deep Relational Reasoning for the Prediction of Language Impairment and Postoperative Seizure Outcome Using Preoperative DWI Connectome Data of Children With Focal Epilepsy","volume":"40","year":"2021","journal-title":"IEEE Trans Med Imaging"},{"key":"2024091822501989000_2024.09.11.612511v1.11","doi-asserted-by":"crossref","unstructured":"Baraban SC (2005) Modeling Epilepsy and Seizures in Developing Zebrafish Larvae. 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