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Here we tested the hypothesis that CP-AMPAR upregulation results from a form of homeostatic plasticity, previously characterized in vitro and in other brain regions, that depends on retinoic acid (RA) signaling in dendrites. Under normal conditions, ongoing synaptic transmission maintains intracellular Ca\n                  <jats:sup>2+<\/jats:sup>\n                  at levels sufficient to suppress RA synthesis. Prolonged blockade of neuronal activity results in disinhibition of RA synthesis, leading to increased GluA1 translation and synaptic insertion of homomeric GluA1 CP-AMPARs. Using slice recordings, we found that increasing RA signaling in NAcc medium spiny neurons (MSN) from drug-na\u00efve rats rapidly upregulates CP-AMPARs, and that this pathway is operative only in MSN expressing the D1 dopamine receptor. In MSN recorded from rats that have undergone incubation of craving, this effect of RA is occluded; instead, interruption of RA signaling in the slice normalizes the incubation-associated elevation of synaptic CP-AMPARs. Paralleling this in vitro finding, interruption of RA signaling in the NAcc of \u2018incubated rats\u2019 normalizes the incubation-associated elevation of cue-induced cocaine seeking. These results suggest that RA signaling becomes tonically active in the NAcc during cocaine withdrawal and, by maintaining elevated CP-AMPAR levels, contributes to the incubation of cocaine craving.\n                <\/jats:p>","DOI":"10.1101\/2024.09.12.611703","type":"posted-content","created":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T01:31:38Z","timestamp":1726450298000},"source":"Crossref","is-referenced-by-count":2,"title":["Retinoic acid-mediated homeostatic plasticity drives cell type-specific CP-AMPAR accumulation in nucleus accumbens core and incubation of cocaine craving"],"prefix":"10.64898","author":[{"given":"Eun-Kyung","family":"Hwang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda M.","family":"Wunsch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina E.","family":"Wolf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024091717050520000_2024.09.12.611703v1.1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02245266"},{"key":"2024091717050520000_2024.09.12.611703v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2004.06.027"},{"issue":"11","key":"2024091717050520000_2024.09.12.611703v1.3","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1001\/jamapsychiatry.2016.2181","article-title":"Incubation of Cue-Induced Craving in Adults Addicted to Cocaine Measured by Electroencephalography","volume":"73","year":"2016","journal-title":"JAMA Psychiatry"},{"key":"2024091717050520000_2024.09.12.611703v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2011.06.001"},{"key":"2024091717050520000_2024.09.12.611703v1.5","doi-asserted-by":"publisher","DOI":"10.1111\/adb.12205"},{"key":"2024091717050520000_2024.09.12.611703v1.6","doi-asserted-by":"crossref","unstructured":"Wolf ME . 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