{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T22:48:38Z","timestamp":1768517318545,"version":"3.49.0"},"posted":{"date-parts":[[2024,9,22]]},"group-title":"Physiology","reference-count":51,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,9,22]],"date-time":"2024-09-22T00:00:00Z","timestamp":1726963200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,9,22]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:sec id=\"s21\">\n                  <jats:title>Background &amp; Aims<\/jats:title>\n                  <jats:p>Intestinal tuft cells have recently been the interest of studies in several human gastrointestinal diseases. However, the impact of tuft cell deletion on intestinal physiological functions are not fully understood. This study investigated the effects of acute tuft cell loss on nutrient absorption and cell lineage differentiation.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec id=\"s22\">\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>\n                    Tuft cell deletion was induced in\n                    <jats:italic>DCLK1-IRES-GFP-CreERT2\/+;Rosa-DTA<\/jats:italic>\n                    (DCLK1-DTA) mice by a single tamoxifen injection concomitant with littermate controls. Intestinal tissues were analyzed two-, four-, or seven-days post tamoxifen injection.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec id=\"s23\">\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>\n                    DCLK1-DTA mice showed significantly shortened small intestinal length and body weight loss on day 4. Impaired activities of Na\n                    <jats:sup>+<\/jats:sup>\n                    -dependent glucose transporter 1 (SGLT1) and cystic fibrosis transmembrane regulator (CFTR) were observed in Ussing chamber experiments. Tissue immunostaining revealed a transient deletion of intestinal and biliary tuft cells, which was maximal on day 4 and recovered by day 7. On day 4 post tamoxifen, cholecystokinin (CCK)+ enteroendocrine cell numbers were increased particularly in the ileum. Correlated with the tuft cell reduction, the frequency of mislocalized Paneth cells, which were co-labeled by Paneth and goblet cell markers, was increased in the villus regions. In the lamina propria, fewer mast cells and leukocytes were found in the day 4 DCLK1-DTA mice than in controls.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec id=\"s24\">\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Ablation of intestinal tuft cells may induce nutrient malabsorption through alterations in epithelial cell proliferation and differentiation along with changes in mucosal defense response. These observations elucidate a new role for tuft cells in regulating intestinal absorption and mucosal regeneration.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2024.09.18.613746","type":"posted-content","created":{"date-parts":[[2024,9,22]],"date-time":"2024-09-22T16:50:17Z","timestamp":1727023817000},"source":"Crossref","is-referenced-by-count":0,"title":["Acute tuft cell ablation induces malabsorption and alterations in secretory and immune cell lineages in small intestine"],"prefix":"10.64898","author":[{"given":"Michael","family":"Momoh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisca","family":"Adeniran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia","family":"Ramos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5749-8394","authenticated-orcid":false,"given":"Kathleen E.","family":"DelGiorno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Seno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph T.","family":"Roland","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5395-0497","authenticated-orcid":false,"given":"Izumi","family":"Kaji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024092601151539000_2024.09.18.613746v1.1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.314815.118"},{"key":"2024092601151539000_2024.09.18.613746v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2820"},{"key":"2024092601151539000_2024.09.18.613746v1.3","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201010127"},{"key":"2024092601151539000_2024.09.18.613746v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-018-0237-x"},{"key":"2024092601151539000_2024.09.18.613746v1.5","doi-asserted-by":"crossref","unstructured":"McKinley ET , Sui Y , Al-Kofahi Y , Millis BA , Tyska MJ , Roland JT , et al. 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