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Corneal stromal stem cells (CSSCs) have the potential to address this global shortage by allowing a single donor cornea to treat multiple patients. To directly deliver CSSCs to corneal defects within an engineered biomatrix, we developed a UNIversal Orthogonal Network (UNION) collagen bioink that crosslinks\n                  <jats:italic>in situ<\/jats:italic>\n                  with a bioorthogonal, covalent chemistry. This cell-gel therapy is optically transparent, stable against contraction forces exerted by CSSCs, and permissive to the efficient growth of corneal epithelial cells. Furthermore, CSSCs remain viable within the UNION collagen gel precursor solution under standard storage and transportation conditions. This approach promoted corneal transparency and re-epithelialization in a rabbit anterior lamellar keratoplasty model, indicating that the UNION collagen bioink serves effectively as an\n                  <jats:italic>in situ<\/jats:italic>\n                  -forming, suture-free therapy for delivering CSSCs to corneal wounds.\n                <\/jats:p>\n                <jats:p>\n                  <jats:bold>TEASER.<\/jats:bold>\n                  Corneal stem cells are delivered within chemically crosslinked collagen as a transparent, regenerative biomaterial therapy.\n                <\/jats:p>","DOI":"10.1101\/2024.09.19.613997","type":"posted-content","created":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T11:50:13Z","timestamp":1727178613000},"source":"Crossref","is-referenced-by-count":1,"title":["In Situ UNIversal Orthogonal Network (UNION) Bioink Deposition for Direct Delivery of Corneal Stromal Stem Cells to Corneal Wounds"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0327-5635","authenticated-orcid":false,"given":"Lucia G.","family":"Brunel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2160-4167","authenticated-orcid":false,"given":"Betty","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3741-654X","authenticated-orcid":false,"given":"Sarah M.","family":"Hull","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7077-9676","authenticated-orcid":false,"given":"Uiyoung","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3461-8015","authenticated-orcid":false,"given":"Thitima","family":"Wungcharoen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9934-5310","authenticated-orcid":false,"given":"Gabriella Maria","family":"Fernandes-Cunha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youngyoon Amy","family":"Seo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1652-3781","authenticated-orcid":false,"given":"Patrik K.","family":"Johansson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9801-6304","authenticated-orcid":false,"given":"Sarah C.","family":"Heilshorn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7980-3070","authenticated-orcid":false,"given":"David","family":"Myung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024092805001835000_2024.09.19.613997v1.1","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1001\/jamaophthalmol.2015.4776","article-title":"Global Survey of Corneal Transplantation and Eye Banking","volume":"134","year":"2016","journal-title":"JAMA Ophthalmology"},{"key":"2024092805001835000_2024.09.19.613997v1.2","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.1097\/ICO.0000000000001666","article-title":"Etiology of Global Corneal Blindness and Current Practices of Corneal Transplantation: A Focused Review","volume":"37","year":"2018","journal-title":"Cornea"},{"key":"2024092805001835000_2024.09.19.613997v1.3","doi-asserted-by":"publisher","DOI":"10.1002\/stem.91"},{"key":"2024092805001835000_2024.09.19.613997v1.4","doi-asserted-by":"publisher","DOI":"10.1002\/sctm.19-0306"},{"key":"2024092805001835000_2024.09.19.613997v1.5","doi-asserted-by":"crossref","unstructured":"S. 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