{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T14:47:04Z","timestamp":1791557224376,"version":"4.3.4"},"posted":{"date-parts":[[2024,10,10]]},"group-title":"Molecular Biology","reference-count":86,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T00:00:00Z","timestamp":1728518400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,10,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  The impact of non-coding RNAs on stem cell biology and differentiation processes is an important and incompletely understood area of research. Using the testes of\n                  <jats:italic>Drosophila melanogaster<\/jats:italic>\n                  as a valuable system for investigating these processes, we identified a polycistronic locus from which two non-coding transcripts,\n                  <jats:italic>miR-317<\/jats:italic>\n                  and the long non-coding RNA (lncRNA)\n                  <jats:italic>Peony<\/jats:italic>\n                  , are produced, with alternative polyadenylation implicated in regulation of their differential expression levels. We report here that each transcript has a distinct role in Drosophila testes; the increased expression of\n                  <jats:italic>Peony<\/jats:italic>\n                  results in the disruption of the muscle sheath covering the testis, and the absence of\n                  <jats:italic>miR-317<\/jats:italic>\n                  leads to the emergence of germ-cell tumors in developing flies. The deficiency of\n                  <jats:italic>miR-317<\/jats:italic>\n                  increases Notch signaling activity in somatic cyst cells, upregulates multiple predicted targets of\n                  <jats:italic>miR-317<\/jats:italic>\n                  , and drives germline tumorigenesis. Our findings establish miR-317 as a tumor suppressor controlling Notch signaling strength and uncover alternative polyadenylation as an unrecognized mechanism governing miRNA expression.\n                <\/jats:p>","DOI":"10.1101\/2024.10.10.617551","type":"posted-content","created":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T22:50:15Z","timestamp":1728600615000},"source":"Crossref","is-referenced-by-count":1,"title":["Alternative polyadenylation of a polycistronic locus regulates the tumor-suppressive miR-317 and the lncRNA Peony in Drosophila"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1608-3368","authenticated-orcid":false,"given":"Travis D.","family":"Carney","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3855-0345","authenticated-orcid":false,"given":"Halyna R.","family":"Shcherbata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025101722401369000_2024.10.10.617551v4.1","doi-asserted-by":"crossref","unstructured":"Albert EA , Puretskaia OA , Terekhanova NV , Labudina A , Bokel C . 2018. 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