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Thus, characteristic emergent rules of collective migration can arise from cell-cell contacts locally tweaking architecture \u2013 orchestrating self-regulation during development, wound healing, and cancer progression. The new\n                  <jats:italic>Drosophila<\/jats:italic>\n                  testis-nascent-myotube-system allows dissection of contact-dependent migration in vivo at high resolution. Here, we describe a process driving gap-closure during migration: Contact-mesenchymalization via the axon guidance factor Plexin A. This is crucial for testis myotubes to migrate as a continuous sheet, allowing normal sculpting-morphogenesis. Cells must stay filopodial and dynamically ECM-tethered near cell-cell contacts to spread while collectively moving. Our data suggest Semaphorin 1B acts as a Plexin A antagonist, fine-tuning activation. Our data reveal a contact-dependent mechanism to maintain sheet-integrity during migration, driving organ-morphogenesis using a highly conserved pathway. This is relevant for understanding mesenchymal organ-sculpting and gap-closure in migratory contexts like angiogenesis.\n                <\/jats:p>","DOI":"10.1101\/2024.10.10.617649","type":"posted-content","created":{"date-parts":[[2024,10,11]],"date-time":"2024-10-11T08:15:15Z","timestamp":1728634515000},"source":"Crossref","is-referenced-by-count":0,"title":["Plexin\/Semaphorin Antagonism Orchestrates Collective Cell Migration, Gap Closure and Organ sculpting by Contact-Mesenchymalization"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6995-8159","authenticated-orcid":false,"given":"Maik C.","family":"Bischoff","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jenevieve E.","family":"Norton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1412-3987","authenticated-orcid":false,"given":"Mark","family":"Peifer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024101405100462000_2024.10.10.617649v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2720"},{"key":"2024101405100462000_2024.10.10.617649v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2015.14"},{"key":"2024101405100462000_2024.10.10.617649v1.3","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201508047"},{"key":"2024101405100462000_2024.10.10.617649v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3438"},{"issue":"1","key":"2024101405100462000_2024.10.10.617649v1.5","doi-asserted-by":"crossref","first-page":"2210","DOI":"10.1038\/s41467-017-02373-8","article-title":"Polarized actin and VE-cadherin dynamics regulate junctional remodelling and cell migration during sprouting angiogenesis","volume":"8","year":"2017","journal-title":"Nature Communications"},{"issue":"9","key":"2024101405100462000_2024.10.10.617649v1.6","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1038\/nphys3040","article-title":"Forces driving epithelial wound healing","volume":"10","year":"2014","journal-title":"Nature Physics"},{"key":"2024101405100462000_2024.10.10.617649v1.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.08.003"},{"issue":"1","key":"2024101405100462000_2024.10.10.617649v1.8","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1038\/s41467-020-20563-9","article-title":"Adhesion-mediated heterogeneous actin organization governs apoptotic cell extrusion","volume":"12","year":"2021","journal-title":"Nature Communications"},{"issue":"6","key":"2024101405100462000_2024.10.10.617649v1.9","first-page":"490","article-title":"Can mesenchymal cells undergo collective cell migration","volume":"5","year":"2011","journal-title":"The case of the neural crest"},{"issue":"11","key":"2024101405100462000_2024.10.10.617649v1.10","doi-asserted-by":"crossref","first-page":"e2100124","DOI":"10.1002\/bies.202100124","article-title":"Collective cell migration driven by filopodia-New insights from the social behavior of myotubes","volume":"43","year":"2021","journal-title":"Bioessays"},{"key":"2024101405100462000_2024.10.10.617649v1.11","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-26351-3"},{"key":"2024101405100462000_2024.10.10.617649v1.12","doi-asserted-by":"crossref","unstructured":"Bischoff, M.C. and S. 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