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Using a major efflux knockout strain, we developed a\n                  <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                  system that enables controlled expression of MexXY-OprM in the absence of background efflux complexes with overlapping substrate profiles. This system identified three groups of potential substrates: substrates, partial substrates, and nonsubstrates, and defined trimethoprim as a new substrate for MexXY-OprM.\n                <\/jats:p>","DOI":"10.1101\/2024.10.18.619101","type":"posted-content","created":{"date-parts":[[2024,10,19]],"date-time":"2024-10-19T04:25:16Z","timestamp":1729311916000},"source":"Crossref","is-referenced-by-count":0,"title":["Determination of\n                  <i>Pseudomonas aeruginosa<\/i>\n                  MexXY-OprM substrate profile in a major efflux knockout system reveals distinct antibiotic substrate classes"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9467-7175","authenticated-orcid":false,"given":"Logan G.","family":"Kavanaugh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shraddha 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