{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T23:18:27Z","timestamp":1768519107862,"version":"3.49.0"},"posted":{"date-parts":[[2024,10,23]]},"group-title":"Immunology","reference-count":59,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,10,23]],"date-time":"2024-10-23T00:00:00Z","timestamp":1729641600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,10,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  The extracellular matrix (ECM) is known to regulate innate immune cells but its role in T cell functions is poorly understood. Here, we show a protective role for ECM proteoglycans, lumican and biglycan in hapten-induced contact dermatitis that is achieved through limiting proinflammatory CD4\n                  <jats:sup>+<\/jats:sup>\n                  T cells. Lumican and biglycan-null mice develop significant inflammation with greater numbers of CD4\n                  <jats:sup>+<\/jats:sup>\n                  T cells in hapten-challenged ear pinnae, while their draining lymph nodes show increased T-bet-STAT1 signaling, Th1 commitment, and IFN-\u03b3 secreting CD4\n                  <jats:sup>+<\/jats:sup>\n                  T cell proliferation. Wild type mouse lymph node fibroblastic reticular cells secrete lumican, biglycan and decorin, a related proteoglycan, while none are expressed by naive or activated T cells.\n                  <jats:italic>In vitro<\/jats:italic>\n                  , lumican and biglycan co-localize with LFA-1 on T cell surfaces, and all three proteoglycans suppress LFA-1 mediated T cell activation. Overall, this study elucidates a novel paracrine regulation of Th1 cells by ECM proteoglycans to limit inflammation and tissue damage.\n                <\/jats:p>","DOI":"10.1101\/2024.10.20.619307","type":"posted-content","created":{"date-parts":[[2024,10,23]],"date-time":"2024-10-23T14:25:15Z","timestamp":1729693515000},"source":"Crossref","is-referenced-by-count":0,"title":["Paracrine regulations of IFN-\u03b3 secreting CD4\n                  <sup>+<\/sup>\n                  T cells by lumican and biglycan are protective in allergic contact dermatitis"],"prefix":"10.64898","author":[{"given":"George","family":"Maiti","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jihane","family":"Frikeche","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia","family":"Loomis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shukti","family":"Chakravarti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024102514200981000_2024.10.20.619307v1.1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.13119"},{"key":"2024102514200981000_2024.10.20.619307v1.2","first-page":"4.2.1","article-title":"Contact Hypersensitivity","volume":"113","year":"2016","journal-title":"Curr Protoc Immunol"},{"key":"2024102514200981000_2024.10.20.619307v1.3","doi-asserted-by":"publisher","DOI":"10.1038\/nri3150"},{"key":"2024102514200981000_2024.10.20.619307v1.4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2009.02082.x"},{"key":"2024102514200981000_2024.10.20.619307v1.5","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2262"},{"key":"2024102514200981000_2024.10.20.619307v1.6","doi-asserted-by":"crossref","unstructured":"G. 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