{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T22:30:54Z","timestamp":1773527454119,"version":"3.50.1"},"posted":{"date-parts":[[2024,10,21]]},"group-title":"Microbiology","reference-count":52,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T00:00:00Z","timestamp":1729468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,10,21]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Cystic fibrosis (CF) is a multi-organ genetic disorder that affects more than 100,000 individuals worldwide. Chronic respiratory infections are among the hallmark complications associated with CF lung disease, and these infections are often due to polymicrobial communities that colonize the airways of persons with CF (pwCF). Such infections are a significant cause of morbidity and mortality, with studies indicating that pwCF who are co-infected with more than one organism experience more frequent pulmonary exacerbations, leading to a faster decline in lung function. Previous work established an\n                  <jats:italic>in vitro<\/jats:italic>\n                  CF-relevant polymicrobial community model composed of\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  ,\n                  <jats:italic>S. aureus<\/jats:italic>\n                  ,\n                  <jats:italic>S. sanguinis<\/jats:italic>\n                  , and\n                  <jats:italic>P. melaninogenica<\/jats:italic>\n                  .\n                  <jats:italic>P. melaninogenica<\/jats:italic>\n                  cannot survive in monoculture in this model. In this study, we leverage this model to investigate the interactions between\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  and\n                  <jats:italic>P. melaninogenica<\/jats:italic>\n                  , allowing us to understand the mechanisms by which the two microbes interact to support the growth of\n                  <jats:italic>P. melaninogenica<\/jats:italic>\n                  specifically in the context of the polymicrobial community. We demonstrate a cross-feeding mechanism whereby\n                  <jats:italic>P. melaninogenica<\/jats:italic>\n                  metabolizes mucin into short-chain fatty acids that are in turn utilized by\n                  <jats:italic>P. aeruginosa<\/jats:italic>\n                  and converted into metabolites (succinate, acetate) that are cross-fed to\n                  <jats:italic>P. melaninogenica<\/jats:italic>\n                  , supporting the survival of this anaerobe in the CF lung-relevant model.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Importance<\/jats:title>\n                  <jats:p>\n                    Polymicrobial interactions impact disease outcomes in pwCF who suffer from chronic respiratory infections. Previous work established a CF-relevant polymicrobial community model that allows experimental probing of these microbial interactions to achieve a better understanding of the factors that govern the mechanisms by which CF lung microbes influence each other. In this study, we investigate the interaction between\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    and\n                    <jats:italic>P. melaninogenica<\/jats:italic>\n                    , which are two highly prevalent and abundant CF lung microbes. We uncover a cross-feeding mechanism that requires the metabolism of mucin by\n                    <jats:italic>P. melaninogenica<\/jats:italic>\n                    to generate short-chain fatty acids that are cross-fed to\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    , and into metabolized into metabolites which are then cross-fed back to\n                    <jats:italic>P. melaninogenica<\/jats:italic>\n                    to support the growth of this anaerobe.\n                  <\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2024.10.21.619475","type":"posted-content","created":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T23:05:16Z","timestamp":1729551916000},"source":"Crossref","is-referenced-by-count":3,"title":["<i>Pseudomonas aeruginosa<\/i>\n                  supports the survival of\n                  <i>Prevotella melaninogenica<\/i>\n                  in a cystic fibrosis lung polymicrobial community through metabolic cross-feeding"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3277-2948","authenticated-orcid":false,"given":"Bassam","family":"El Hafi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3056-8356","authenticated-orcid":false,"given":"Fabrice","family":"Jean-Pierre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2861-4392","authenticated-orcid":false,"given":"George A.","family":"O\u2019Toole","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024102416500827000_2024.10.21.619475v1.1","unstructured":"Cystic Fibrosis Foundation. About Cystic Fibrosis. https:\/\/www.cff.org\/intro-cf\/about-cystic-fibrosis. Retrieved 30 July 2024."},{"key":"2024102416500827000_2024.10.21.619475v1.2","doi-asserted-by":"crossref","unstructured":"Yahiaoui Y , Jablonski M , Hubert D , Mosnier-Pudar H , Noel L-H , Stern M , Grenet D , Grunfeld J-P , Chauveau D , Fakhouri F . 2009. Renal involvement in cystic fibrosis: Diseases spectrum and clinical relevance. Clinical Journal of the American Society of Nephrology 4.","DOI":"10.2215\/CJN.00750209"},{"key":"2024102416500827000_2024.10.21.619475v1.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.medcle.2023.06.013"},{"key":"2024102416500827000_2024.10.21.619475v1.4","doi-asserted-by":"crossref","unstructured":"Myer H , Chupita S , Jnah A . 2023. Cystic fibrosis: Back to the basics. Neonatal Network 23\u201330.","DOI":"10.1891\/NN-2022-0007"},{"key":"2024102416500827000_2024.10.21.619475v1.5","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra2216474"},{"key":"2024102416500827000_2024.10.21.619475v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.lpm.2023.104169"},{"key":"2024102416500827000_2024.10.21.619475v1.7","unstructured":"2020. Cystic Fibrosis Foundation Patient Registry Annual Data Report 2019. 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