{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T01:40:27Z","timestamp":1772156427811,"version":"3.50.1"},"posted":{"date-parts":[[2024,10,24]]},"group-title":"Microbiology","reference-count":36,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T00:00:00Z","timestamp":1729728000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,1,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  <jats:italic>Chlamydia trachomatis<\/jats:italic>\n                  serovar L2\n                  <jats:italic>(Ct),<\/jats:italic>\n                  an obligate intracellular bacterium that does not encode FtsZ, divides by a polarized budding process. In the absence of FtsZ, we show that FtsK, a chromosomal translocase, is critical for divisome assembly in\n                  <jats:italic>Ct<\/jats:italic>\n                  . Chlamydial FtsK forms discrete foci at the septum and at the base of the progenitor mother cell, and our data indicate that FtsK foci at the base of the mother cell mark the location of nascent divisome complexes that form at the site where a daughter cell will emerge in the next round of division. The divisome in\n                  <jats:italic>Ct<\/jats:italic>\n                  has a hybrid composition, containing elements of the divisome and elongasome from other bacteria, and FtsK is recruited to nascent divisomes prior to the other chlamydial divisome proteins assayed, including the PBP2 and PBP3 transpeptidases, and MreB and MreC. Knocking down FtsK prevents divisome assembly in\n                  <jats:italic>Ct<\/jats:italic>\n                  and inhibits cell division and septal peptidoglycan synthesis. We further show that MreB does not function like FtsZ and serve as a scaffold for the assembly of the\n                  <jats:italic>Ct<\/jats:italic>\n                  divisome. Rather, MreB is one of the last proteins recruited to the chlamydial divisome, and it is necessary for the formation of septal peptidoglycan rings. Our studies illustrate the critical role of chlamydial FtsK in coordinating divisome assembly and peptidoglycan synthesis in this obligate intracellular bacterial pathogen.\n                <\/jats:p>","DOI":"10.1101\/2024.10.24.620021","type":"posted-content","created":{"date-parts":[[2024,10,25]],"date-time":"2024-10-25T00:00:15Z","timestamp":1729814415000},"source":"Crossref","is-referenced-by-count":2,"title":["FtsK is Critical for the Assembly of the Unique Divisome Complex of the FtsZ-less\n                  <i>Chlamydia trachomatis<\/i>"],"prefix":"10.64898","author":[{"given":"McKenna","family":"Harpring","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junghoon","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangming","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3721-6839","authenticated-orcid":false,"given":"Scot P.","family":"Ouellette","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6177-0223","authenticated-orcid":false,"given":"John V.","family":"Cox","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025011023200694000_2024.10.24.620021v2.1","doi-asserted-by":"crossref","unstructured":"Abdelrahman, Y. , Ouellette, Scot P. , Belland, Robert J. , Cox, John V. (2016). \u201cPolarized Cell Division of Chlmaydia Trachomatis\u201d PLOS Pathogens","DOI":"10.1371\/journal.ppat.1005866"},{"key":"2025011023200694000_2024.10.24.620021v2.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsre.2005.03.002"},{"key":"2025011023200694000_2024.10.24.620021v2.3","doi-asserted-by":"crossref","unstructured":"Abdelrahman, Y. O. , Scot P. Belland , Robert J. Cox , John V. (2016). \u201cPolarized Cell Division of Chlamydia trachomatis.\u201d PLOS Pathogens.","DOI":"10.1371\/journal.ppat.1005866"},{"key":"2025011023200694000_2024.10.24.620021v2.4","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.111008998"},{"key":"2025011023200694000_2024.10.24.620021v2.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2020.10.013"},{"key":"2025011023200694000_2024.10.24.620021v2.6","doi-asserted-by":"publisher","DOI":"10.1021\/bi900014d"},{"key":"2025011023200694000_2024.10.24.620021v2.7","doi-asserted-by":"publisher","DOI":"10.1126\/science.aak9973"},{"key":"2025011023200694000_2024.10.24.620021v2.8","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)80076-Q"},{"key":"2025011023200694000_2024.10.24.620021v2.9","doi-asserted-by":"crossref","unstructured":"Cox, J. V. A. , Yasser Mohamed . Ouellette, Scot P. 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