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Microtubules emanating from the mitotic spindle pole bind a conserved complex of proteins at the cell cortex which orients the spindle and ultimately the cell division plane. Control of spindle orientation is of particular importance in developing tissues, such as the developing brain. Misorientation of the mitotic spindle and thus subsequent division plane misalignment can contribute to improper segregation of cell fate determinants in developing neuroblasts, leading to a rare neurological disorder known as microcephaly. We demonstrate that the nuclear transport protein importin \u03b1, when palmitoylated, plays a critical role in mitotic spindle orientation through localizing factors, such as NuMA, to the cell cortex. We also observe craniofacial developmental defects in\n                  <jats:italic>Xenopus laevis<\/jats:italic>\n                  when importin \u03b1 palmitoylation is abrogated, including smaller head and brains, a hallmark of spindle misorientation and microcephaly. These findings characterize not only a role for importin \u03b1 in spindle orientation, but also a broader role for importin \u03b1 palmitoylation which has significance for many cellular processes.\n                <\/jats:p>","DOI":"10.1101\/2024.10.25.620315","type":"posted-content","created":{"date-parts":[[2024,10,26]],"date-time":"2024-10-26T03:25:24Z","timestamp":1729913124000},"source":"Crossref","is-referenced-by-count":1,"title":["Palmitoylated Importin \u03b1 Regulates Mitotic Spindle Orientation Through Interaction with NuMA"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4954-4204","authenticated-orcid":false,"given":"Patrick James","family":"Sutton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3860-6759","authenticated-orcid":false,"given":"Natalie","family":"Mosqueda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1045-4033","authenticated-orcid":false,"given":"Christopher W.","family":"Brownlee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025020923452001000_2024.10.25.620315v2.1","doi-asserted-by":"publisher","DOI":"10.1002\/cpim.58.Proximity"},{"key":"2025020923452001000_2024.10.25.620315v2.2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyad036"},{"key":"2025020923452001000_2024.10.25.620315v2.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2024.05.022"},{"key":"2025020923452001000_2024.10.25.620315v2.4","doi-asserted-by":"publisher","DOI":"10.1038\/75556"},{"key":"2025020923452001000_2024.10.25.620315v2.5","doi-asserted-by":"publisher","DOI":"10.1242\/dev.140764"},{"key":"2025020923452001000_2024.10.25.620315v2.6","doi-asserted-by":"publisher","DOI":"10.1242\/dev.135475"},{"key":"2025020923452001000_2024.10.25.620315v2.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2014.06.014"},{"key":"2025020923452001000_2024.10.25.620315v2.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2006.05.005"},{"key":"2025020923452001000_2024.10.25.620315v2.9","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm066"},{"key":"2025020923452001000_2024.10.25.620315v2.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.12.001"},{"key":"2025020923452001000_2024.10.25.620315v2.11","doi-asserted-by":"crossref","unstructured":"Camuglia, J. , S. 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