{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:09:59Z","timestamp":1792786199843,"version":"4.5.0"},"posted":{"date-parts":[[2024,10,31]]},"group-title":"Microbiology","reference-count":38,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,10,31]],"date-time":"2024-10-31T00:00:00Z","timestamp":1730332800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"award":["HA6013\/4-1 project #393153343"],"award-info":[{"award-number":["HA6013\/4-1 project #393153343"]}],"id":[{"id":"https:\/\/ror.org\/018mejw64","id-type":"ROR","asserted-by":"publisher"}]},{"award":["DFG HA 6013\/10-1 project #512328936"],"award-info":[{"award-number":["DFG HA 6013\/10-1 project #512328936"]}],"id":[{"id":"https:\/\/ror.org\/018mejw64","id-type":"ROR","asserted-by":"publisher"}]},{"award":["DFG HA 6013\/11-1 project #530009014"],"award-info":[{"award-number":["DFG HA 6013\/11-1 project #530009014"]}],"id":[{"id":"https:\/\/ror.org\/018mejw64","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Wilhelm Sander Stiftung","award":["2019.027.1"],"award-info":[{"award-number":["2019.027.1"]}],"id":[{"id":"https:\/\/ror.org\/02q83sc19","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Cancer Institute","award":["75N91024F00011"],"award-info":[{"award-number":["75N91024F00011"]}]},{"name":"National Cancer Institute","award":["R01 CA239591"],"award-info":[{"award-number":["R01 CA239591"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,28]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Kaposi\u2019s sarcoma-associated herpesvirus (KSHV) is a human tumor virus. Common marmosets, a potential non-human primate model for vaccine development and infection studies, have previously been shown to support KSHV infection. We established infection of marmosets with BAC-derived, recombinant KSHV to analyze the role of individual glycoproteins (GPs) in tissue tropism. Three groups of four animals were infected intravenously with BAC-derived KSHV wild type (wt), EphA2-receptor-binding-deficient KSHV gH\n                  <jats:sup>ASAELAAN<\/jats:sup>\n                  , or KSHV\u0394K8.1, lacking glycoprotein K8.1. Viral DNA was detected in multiple tissues of all animals, with the highest loads in spleen, liver, heart, and bone marrow. In most tissues, viral DNA loads did not significantly differ between groups. However, KSHV\u0394K8.1-infected animals exhibited significantly lower KSHV DNA levels in liver and bone marrow. KSHV wt infection induced activation of CD4\n                  <jats:sup>+<\/jats:sup>\n                  and CD8\n                  <jats:sup>+<\/jats:sup>\n                  T-cells. In animals infected with KSHV wt, KSHV gH\n                  <jats:sup>ASAELAAN<\/jats:sup>\n                  or KSHV\u0394K8.1, different B cell subpopulations expanded after infection, which suggests that the glycoproteins themselves or the cell tropism imparted by their presence or absence might shape the host response to KSHV. In summary, our findings demonstrate that neither the Eph receptor interaction nor GP K8.1 is essential for intravenous infection, but K8.1 critically contributes to KSHV tissue tropism in vivo.\n                <\/jats:p>","DOI":"10.1101\/2024.10.31.621273","type":"posted-content","created":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:14Z","timestamp":1730419214000},"source":"Crossref","is-referenced-by-count":0,"title":["Glycoprotein K8.1 shapes KSHV liver and bone marrow tropism in the marmoset infection model"],"prefix":"10.64898","author":[{"given":"Christiane","family":"Stahl-Hennig","sequence":"first","affiliation":[{"name":"German Primate Center \u2013 Leibniz Institute for Primate Research, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Berit","family":"Roshani","sequence":"additional","affiliation":[{"name":"German Primate Center \u2013 Leibniz Institute for Primate Research, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna K.","family":"Gro\u00dfkopf","sequence":"additional","affiliation":[{"name":"German Primate Center \u2013 Leibniz Institute for Primate Research, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Schlagowski","sequence":"additional","affiliation":[{"name":"German Primate Center \u2013 Leibniz Institute for Primate Research, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel Alberto","family":"M\u00e9rida Ru\u00edz","sequence":"additional","affiliation":[{"name":"German Primate Center \u2013 Leibniz Institute for Primate Research, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramona","family":"Vestweber","sequence":"additional","affiliation":[{"name":"German Primate Center \u2013 Leibniz Institute for Primate Research, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Eymard","sequence":"additional","affiliation":[{"name":"Department of Interdisciplinary Oncology, Stanley S. Scott Cancer Center, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Wood","sequence":"additional","affiliation":[{"name":"Department of Interdisciplinary Oncology, Stanley S. Scott Cancer Center, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanchuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Harald zur Hausen Institute of Virology, Universit\u00e4tsklinikum Erlangen, Friedrich-Alexander-Universit\u00e4t Erlangen-N\u00fcrnberg, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Bleyer","sequence":"additional","affiliation":[{"name":"German Primate Center \u2013 Leibniz Institute for Primate Research, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Stolte-Leeb","sequence":"additional","affiliation":[{"name":"German Primate Center \u2013 Leibniz Institute for Primate Research, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"P\u00f6hlmann","sequence":"additional","affiliation":[{"name":"German Primate Center \u2013 Leibniz Institute for Primate Research, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise","family":"Whitby","sequence":"additional","affiliation":[{"name":"Frederick National Laboratory for Cancer Research, Viral Oncology Section, AIDS and Cancer Virus Program, Frederick, MD, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8873-3863","authenticated-orcid":false,"given":"Armin","family":"Ensser","sequence":"additional","affiliation":[{"name":"Harald zur Hausen Institute of Virology, Universit\u00e4tsklinikum Erlangen, Friedrich-Alexander-Universit\u00e4t Erlangen-N\u00fcrnberg, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4171-2744","authenticated-orcid":false,"given":"Alexander S.","family":"Hahn","sequence":"additional","affiliation":[{"name":"German Primate Center \u2013 Leibniz Institute for Primate Research, G\u00f6ttingen, Germany"},{"name":"Harald zur Hausen Institute of Virology, Universit\u00e4tsklinikum Erlangen, Friedrich-Alexander-Universit\u00e4t Erlangen-N\u00fcrnberg, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026093008551326000_2024.10.31.621273v2.1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7997879"},{"key":"2026093008551326000_2024.10.31.621273v2.2","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-019-0118-8"},{"key":"2026093008551326000_2024.10.31.621273v2.3","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21492"},{"key":"2026093008551326000_2024.10.31.621273v2.4","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-200105000-00009"},{"key":"2026093008551326000_2024.10.31.621273v2.5","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0b013e3283031a8a"},{"key":"2026093008551326000_2024.10.31.621273v2.6","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1097\/COH.0000000000000330","article-title":"Kaposi sarcoma herpesvirus-associated cancers and related diseases","volume":"12","year":"2017","journal-title":"Current Opinion in HIV and AIDS"},{"key":"2026093008551326000_2024.10.31.621273v2.7","doi-asserted-by":"crossref","unstructured":"7. 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