{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T22:45:04Z","timestamp":1768517104626,"version":"3.49.0"},"posted":{"date-parts":[[2024,11,1]]},"group-title":"Developmental Biology","reference-count":64,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,11,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Transcription factor AP-2 gamma (\n                  <jats:bold>TFAP2C<\/jats:bold>\n                  ) has been identified as a key regulator of the trophoblast cell lineage and hemochorial placentation. The rat possesses deep placentation characterized by extensive intrauterine trophoblast cell invasion, which resembles human placentation.\n                  <jats:italic>Tfap2c is<\/jats:italic>\n                  expressed in multiple trophoblast cell lineages, including invasive trophoblast cells situated within the uterine-placental interface of the rat placentation site. Global genome-editing was used to explore the biology of\n                  <jats:italic>Tfap2c<\/jats:italic>\n                  in rat placenta development. Homozygous global disruption of\n                  <jats:italic>Tfap2c<\/jats:italic>\n                  resulted in prenatal lethality. Heterozygous global disruption of\n                  <jats:italic>Tfap2c<\/jats:italic>\n                  was associated with diminished invasive trophoblast cell infiltration into the uterus. The role of TFAP2C in the invasive trophoblast cell lineage was explored using Cre-lox conditional mutagenesis. Invasive trophoblast cell-specific disruption of\n                  <jats:italic>Tfap2c<\/jats:italic>\n                  resulted in inhibition of intrauterine trophoblast cell invasion and intrauterine and postnatal growth restriction. The invasive trophoblast cell lineage was not impaired following conditional monoallelic disruption of\n                  <jats:italic>Tfap2c<\/jats:italic>\n                  . In summary, TFAP2C contributes to the progression of distinct stages of placental development. TFAP2C is a driver of early events in trophoblast cell development and reappears later in gestation as an essential regulator of the invasive trophoblast cell lineage. A subset of TFAP2C actions on trophoblast cells are dependent on gene dosage.\n                <\/jats:p>","DOI":"10.1101\/2024.10.31.621324","type":"posted-content","created":{"date-parts":[[2024,11,2]],"date-time":"2024-11-02T00:10:21Z","timestamp":1730506221000},"source":"Crossref","is-referenced-by-count":0,"title":["TFAP2C is a key regulator of intrauterine trophoblast cell invasion and deep hemochorial placentation"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3540-4411","authenticated-orcid":false,"given":"Esteban M.","family":"Dominguez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6810-6475","authenticated-orcid":false,"given":"Ayelen","family":"Moreno-Irusta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regan L.","family":"Scott","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6742-4591","authenticated-orcid":false,"given":"Khursheed","family":"Iqbal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Soares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024112100151435000_2024.10.31.621324v2.1","doi-asserted-by":"publisher","DOI":"10.1053\/plac.2001.0738"},{"key":"2024112100151435000_2024.10.31.621324v2.2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100814-125620"},{"key":"2024112100151435000_2024.10.31.621324v2.3","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00029.2015"},{"key":"2024112100151435000_2024.10.31.621324v2.4","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402660608"},{"key":"2024112100151435000_2024.10.31.621324v2.5","doi-asserted-by":"publisher","DOI":"10.1530\/REP-16-0325"},{"key":"2024112100151435000_2024.10.31.621324v2.6","unstructured":"Hertig AT ( Arthur T. 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