{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T02:01:59Z","timestamp":1791597719651,"version":"4.3.4"},"posted":{"date-parts":[[2024,11,8]]},"group-title":"Plant Biology","reference-count":47,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T00:00:00Z","timestamp":1731024000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,11,8]]},"abstract":"<jats:title>Summary<\/jats:title>\n                <jats:p>\n                  Plants activate induced defenses through the recognition of molecular patterns. Like pathogen-associated molecular patterns (PAMPs), herbivore-associated molecular patterns (HAMPs) can be recognized by cell surface pattern recognition receptors leading to defensive transcriptional changes in host plants. Herbivore-induced defensive outputs are regulated by the circadian clock, but the underlying molecular mechanisms remain unknown. To investigate how the plant circadian clock regulates transcriptional reprogramming of a specific HAMP-induced pathway, we characterized the daytime and nighttime transcriptional response to caterpillar-derived In11 peptide, in the legume crop cowpea (\n                  <jats:italic>Vigna unguiculata<\/jats:italic>\n                  ). Using diurnal and free-running conditions, we found that daytime In11 elicitation resulted in stronger late-induced gene expression than nighttime. Plants with a conditional arrhythmic phenotype in constant light (LL) conditions lost time-of-day dependent responses to In11 treatment, and this was associated with arrhythmic expression of circadian clock core transcription factor\n                  <jats:italic>Late Elongated Hypocotyl VuLHY1<\/jats:italic>\n                  and\n                  <jats:italic>VuLHY2<\/jats:italic>\n                  . Reporter assays with VuLHY homologs indicated that they interact with the promoter of daytime In11-induced\n                  <jats:italic>Kunitz Trypsin Inhibitor<\/jats:italic>\n                  (\n                  <jats:italic>VuKTI<\/jats:italic>\n                  ) via a canonical and a polymorphic CCA1\/LHY Binding Site (CBS), consistent with a mechanism of direct regulation by circadian clock transcription factors. This study improves our understanding of the time-dependent mechanisms that regulate herbivore-induced gene expression.\n                <\/jats:p>","DOI":"10.1101\/2024.11.06.622352","type":"posted-content","created":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T13:15:18Z","timestamp":1731071718000},"source":"Crossref","is-referenced-by-count":2,"title":["The circadian clock regulates receptor-mediated immune responses to an herbivore-associated molecular pattern"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3403-6661","authenticated-orcid":false,"given":"Natalia","family":"Guayaz\u00e1n 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