{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"medRxiv"}],"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T13:13:47Z","timestamp":1768569227242,"version":"3.49.0"},"posted":{"date-parts":[[2024,11,25]]},"group-title":"Infectious Diseases (except HIV\/AIDS)","reference-count":32,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T00:00:00Z","timestamp":1732492800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,11,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  <jats:italic>Mycoplasma genitalium<\/jats:italic>\n                  is a sexually transmitted pathogen, which can cause a range of reproductive tract diseases in both men and women. To disentangle the relationship between\n                  <jats:italic>M. genitalium<\/jats:italic>\n                  infection(s) and subsequent reproductive health complications at the population level, accurate serological tools are needed. The major challenge in developing specific\n                  <jats:italic>M. genitalium<\/jats:italic>\n                  serological tests is the extensive cross-reactivity with the closely related ubiquitous respiratory tract pathogen,\n                  <jats:italic>M. pneumoniae<\/jats:italic>\n                  . In this report, we describe the development of an immunoblot assay based on a recombinant fragment of the\n                  <jats:italic>M. genitalium<\/jats:italic>\n                  MG075 protein present in lipid-associated membrane extracts. A sensitivity of 87.1% was achieved based on testing antibody responses in sera from 101 adults with PCR-confirmed\n                  <jats:italic>M. genitalium<\/jats:italic>\n                  infection. A specificity of 95.2% was obtained through evaluation of sera from 166 children under 15 years of age with and without\n                  <jats:italic>M. pneumoniae<\/jats:italic>\n                  infection, who were unlikely to have been exposed to sexually transmitted\n                  <jats:italic>M. genitalium<\/jats:italic>\n                  . The development of a serological assay capable of accurately distinguishing\n                  <jats:italic>M. genitalium<\/jats:italic>\n                  and\n                  <jats:italic>M. pneumoniae<\/jats:italic>\n                  , will enable a better understanding of associations between\n                  <jats:italic>M. genitalium<\/jats:italic>\n                  and adverse reproductive sequelae.\n                <\/jats:p>","DOI":"10.1101\/2024.11.19.24317541","type":"posted-content","created":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T11:25:34Z","timestamp":1732533934000},"source":"Crossref","is-referenced-by-count":0,"title":["Detection of human IgG antibodies against\n                  <i>Mycoplasma genitalium<\/i>\n                  using a recombinant MG075 antigen"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5150-1153","authenticated-orcid":false,"given":"Anna Overgaard","family":"Kildemoes","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivia Sureya Seier\u00f8","family":"Rai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth Probst Lyng","family":"Westermann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrik Frederik Bekkevold","family":"Johansen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Bj\u00f8rnelius","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carin","family":"Anagrius","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ida","family":"Rosenkrands","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f8rgen Skov","family":"Jensen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024112702102806000_2024.11.19.24317541v1.1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00006-11"},{"key":"2024112702102806000_2024.11.19.24317541v1.2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ312"},{"key":"2024112702102806000_2024.11.19.24317541v1.3","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(81)92461-2"},{"key":"2024112702102806000_2024.11.19.24317541v1.4","doi-asserted-by":"crossref","first-page":"e0079021","DOI":"10.1128\/jcm.00790-21","article-title":"Latest Advances in Laboratory Detection of Mycoplasma genitalium","volume":"61","year":"2023","journal-title":"J Clin 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