{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T01:40:32Z","timestamp":1772156432328,"version":"3.50.1"},"posted":{"date-parts":[[2024,11,25]]},"group-title":"Microbiology","reference-count":75,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T00:00:00Z","timestamp":1732492800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,2,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Lyme disease, caused by\n                  <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                  , is the most common tick-borne infection in the United States. Arthritis is a major clinical manifestation of infection, and synovial tissue damage has been attributed to the excessive pro-inflammatory responses. The secretory leukocyte protease inhibitor (SLPI) promotes tissue repair and exerts anti-inflammatory effects. The role of SLPI in the development of Lyme arthritis in C57BL\/6 mice, which can be infected with\n                  <jats:italic>B. burgdorferi<\/jats:italic>\n                  , but only develop mild joint inflammation, was therefore examined.\n                  <jats:italic>SLPI<\/jats:italic>\n                  -deficient C57BL\/6 mice challenged with\n                  <jats:italic>B. burgdorferi<\/jats:italic>\n                  had a higher infection load in the tibiotarsal joints and marked periarticular swelling, compared to infected wild type control mice. The ankle joint tissues of\n                  <jats:italic>B. burgdorferi-<\/jats:italic>\n                  infected\n                  <jats:italic>SLPI<\/jats:italic>\n                  -deficient mice contained significantly higher percentages of infiltrating neutrophils and macrophages.\n                  <jats:italic>B. burgdorferi<\/jats:italic>\n                  -infected\n                  <jats:italic>SLPI<\/jats:italic>\n                  -deficient mice also exhibited elevated serum levels of IL-6, neutrophil elastase, and MMP-8. Moreover, using a recently developed BASEHIT (\n                  <jats:bold>BA<\/jats:bold>\n                  cterial\n                  <jats:bold>S<\/jats:bold>\n                  election to\n                  <jats:bold>E<\/jats:bold>\n                  lucidate\n                  <jats:bold>H<\/jats:bold>\n                  ost-microbe\n                  <jats:bold>I<\/jats:bold>\n                  nteractions in high\n                  <jats:bold>T<\/jats:bold>\n                  hroughput) library, we found that SLPI directly interacts with\n                  <jats:italic>B. burgdorferi<\/jats:italic>\n                  . These data demonstrate the importance of SLPI in suppressing periarticular joint inflammation in Lyme disease.\n                <\/jats:p>","DOI":"10.1101\/2024.11.24.625079","type":"posted-content","created":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T23:41:45Z","timestamp":1732578105000},"source":"Crossref","is-referenced-by-count":0,"title":["Secretory leukocyte protease inhibitor influences periarticular joint inflammation in\n                  <i>B. burgdorferi<\/i>\n                  -infected mice"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2396-2128","authenticated-orcid":false,"given":"Qian","family":"Yu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaotian","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Hart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Homer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexia A.","family":"Belperron","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda K.","family":"Bockenstedt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aaron","family":"Ring","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erol","family":"Fikrig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2025031301210136000_2024.11.24.625079v2.1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2702.202731"},{"key":"2025031301210136000_2024.11.24.625079v2.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2022.03.004"},{"key":"2025031301210136000_2024.11.24.625079v2.3","first-page":"563","article-title":"Lyme arthritis","volume":"36","year":"2022","journal-title":"Infectious Disease Clinics"},{"key":"2025031301210136000_2024.11.24.625079v2.4","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.ss6622a1"},{"key":"2025031301210136000_2024.11.24.625079v2.5","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-107-5-725"},{"key":"2025031301210136000_2024.11.24.625079v2.6","doi-asserted-by":"publisher","DOI":"10.1097\/RHU.0000000000001513"},{"key":"2025031301210136000_2024.11.24.625079v2.7","doi-asserted-by":"publisher","DOI":"10.1038\/s41584-021-00648-5"},{"key":"2025031301210136000_2024.11.24.625079v2.8","doi-asserted-by":"publisher","DOI":"10.1002\/art.22441"},{"key":"2025031301210136000_2024.11.24.625079v2.9","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12992"},{"key":"2025031301210136000_2024.11.24.625079v2.10","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.160.2.1022"},{"key":"2025031301210136000_2024.11.24.625079v2.11","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12954"},{"key":"2025031301210136000_2024.11.24.625079v2.12","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/162.1.133"},{"key":"2025031301210136000_2024.11.24.625079v2.13","unstructured":"Barthold, S.W. 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