{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"medRxiv"}],"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T16:11:24Z","timestamp":1772035884740,"version":"3.50.1"},"posted":{"date-parts":[[2024,11,30]]},"group-title":"Neurology","reference-count":57,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,11,30]],"date-time":"2024-11-30T00:00:00Z","timestamp":1732924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.medrxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,11,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>Reliable, circulating biomarkers for Duchenne, Becker and facioscapulohumeral muscular dystrophies (DBMD and FSHD) remain unvalidated. Here, we investigated the plasma extracellular vesicle (EV) proteome to identify disease-specific biomarkers that could accelerate therapy approvals.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We extracted EVs from the plasma of DBMD and FSHD patients and healthy controls using size-exclusion chromatography, conducted mass spectrometry on the extracted EV proteins, and performed comparative analysis to identify disease-specific biomarkers. We correlated the levels of these biomarkers with clinical outcome measures and confounding factors.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The muscle-associated proteins PYGM, MYOM3, FLNC, MYH2 and TTN were exclusively present in DBMD EVs. PYGM, MYOM3, and TTN negatively correlated with age. PYGM and MYOM3 levels were elevated in patients without cardiomyopathy, and PYGM levels were specifically elevated in ambulatory DMD patients. On the other hand, female FSHD patients displayed significantly higher MBL2 and lower GPLD1 levels. However, male FSHD patients exhibited higher C9 and lower C4BPB levels. Additionally, desmosome proteins JUP and DSP were uniquely found in FSHD males. MBL2 positively correlated with age and C4BPB negatively correlated with FSHD severity in male patients.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Interpretation<\/jats:title>\n                  <jats:p>Our findings underscore the sensitivity of analyzing circulating EV content to identify disease-specific protein biomarkers for DBMD and FSHD. Our results also emphasize the potential of EV-based biomarker discovery as a promising approach to monitor disease progression as well as effectiveness of therapies in muscular dystrophy, potentially contributing to their approval. Further research with larger cohorts is needed to validate these biomarkers and explore their clinical implications.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2024.11.29.24317861","type":"posted-content","created":{"date-parts":[[2024,11,30]],"date-time":"2024-11-30T12:15:41Z","timestamp":1732968941000},"source":"Crossref","is-referenced-by-count":2,"title":["Identification of disease-specific extracellular vesicle-associated plasma protein biomarkers for Duchenne Muscular Dystrophy and Facioscapulohumeral Muscular Dystrophy"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3935-0302","authenticated-orcid":false,"given":"Mustafa Bilal","family":"Bayazit","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Don","family":"Henderson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7114-6395","authenticated-orcid":false,"given":"Kim Truc","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo","family":"Re\u00e1tegui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rabi","family":"Tawil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6440-3376","authenticated-orcid":false,"given":"Kevin M.","family":"Flanigan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott Q.","family":"Harper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5453-1152","authenticated-orcid":false,"given":"Nizar Y.","family":"Saad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024120212250947000_2024.11.29.24317861v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-021-00248-3"},{"issue":"1","key":"2024120212250947000_2024.11.29.24317861v1.2","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1159\/000512647","article-title":"Duchenne and Becker Muscular Dystrophies\u2019 Prevalence in MD STARnet Surveillance Sites: An Examination of Racial and Ethnic Differences","volume":"55","year":"2021","journal-title":"Neuroepidemiology"},{"key":"2024120212250947000_2024.11.29.24317861v1.3","doi-asserted-by":"publisher","DOI":"10.1002\/ana.23528"},{"key":"2024120212250947000_2024.11.29.24317861v1.4","doi-asserted-by":"publisher","DOI":"10.1002\/humu.22758"},{"key":"2024120212250947000_2024.11.29.24317861v1.5","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-022-00762-2"},{"key":"2024120212250947000_2024.11.29.24317861v1.6","doi-asserted-by":"crossref","unstructured":"Wilton-Clark, H. and T. 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