{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:57:56Z","timestamp":1785412676446,"version":"3.56.0"},"posted":{"date-parts":[[2024,12,4]]},"group-title":"Cell Biology","reference-count":87,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,12,4]],"date-time":"2024-12-04T00:00:00Z","timestamp":1733270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["R01NS105621"],"award-info":[{"award-number":["R01NS105621"]}],"id":[{"id":"https:\/\/ror.org\/01s5ya894","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01NS129219"],"award-info":[{"award-number":["R01NS129219"]}],"id":[{"id":"https:\/\/ror.org\/01s5ya894","id-type":"ROR","asserted-by":"publisher"}]},{"award":["AL170061(W81XWH1810684)"],"award-info":[{"award-number":["AL170061(W81XWH1810684)"]}],"id":[{"id":"https:\/\/ror.org\/0447fe631","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,10,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Different muscles exhibit varied susceptibility to degeneration in Amyotrophic Lateral Sclerosis (ALS), a fatal neuromuscular disorder. Extraocular muscles (EOMs) are particularly resistant to ALS progression, and exploring the underlying molecular nature may offer significant therapeutic value. Reactive aldehyde 4-hydroxynonenal (HNE) is implicated in ALS pathogenesis, and Aldh3a1 is an inactivation-resistant intracellular aldehyde dehydrogenase that detoxifies 4-HNE to protect eyes against UV-induced oxidative stress. We detected prominently higher levels of\n                  <jats:italic>Aldh3a1<\/jats:italic>\n                  in mouse EOMs compared to other muscles under normal physiological conditions. In an ALS mouse model (hSOD1\n                  <jats:sup>G93A<\/jats:sup>\n                  ) reaching end-stage,\n                  <jats:italic>Aldh3a1<\/jats:italic>\n                  expression was maintained high in EOMs, substantially elevated in soleus and diaphragm, but only moderately increased in extensor digitorum longus (EDL) muscle, which endured the most severe pathological remodeling, as demonstrated by unparalleled upregulation of a denervation marker\n                  <jats:italic>Ankrd1<\/jats:italic>\n                  . Importantly, sciatic nerve transection in wildtype mice further confirmed induced\n                  <jats:italic>Aldh3a1<\/jats:italic>\n                  and\n                  <jats:italic>Ankrd1<\/jats:italic>\n                  expression in an inverse manner across muscle types in response to denervation. Mechanistically, whole-muscle RNA-Seq and pharmacological tests indicate that higher basal levels of lipid oxidation in soleus and diaphragm muscles may predispose them to stronger Nrf2 antioxidant responses under pathological stress compared to EDL, leading to more prominent\n                  <jats:italic>Aldh3a1<\/jats:italic>\n                  upregulation. Additionally, the identification of the myoblast fusion marker\n                  <jats:italic>Mymk<\/jats:italic>\n                  as an EOM signature gene suggests that the spontaneous activation of satellite cells contributes to high levels of\n                  <jats:italic>Aldh3a1<\/jats:italic>\n                  in EOMs. Functionally, adeno-associated virus-mediated overexpression of\n                  <jats:italic>Aldh3a1<\/jats:italic>\n                  protected myotubes from 4-HNE-induced DNA fragmentation and plasma membrane leakage. It also restored MG53-mediated membrane repair, highlighting its potential for clinical applications.\n                <\/jats:p>","DOI":"10.1101\/2024.12.02.626422","type":"posted-content","created":{"date-parts":[[2024,12,4]],"date-time":"2024-12-04T12:50:11Z","timestamp":1733316611000},"source":"Crossref","is-referenced-by-count":0,"title":["Aldh3a1-mediated detoxification of reactive aldehydes contributes to distinct muscle responses to Amyotrophic Lateral Sclerosis progression"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8784-4702","authenticated-orcid":false,"given":"Ang","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuejun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianxun","family":"Yi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianjie","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5684-7177","authenticated-orcid":false,"given":"Jingsong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025102702150437000_2024.12.02.626422v2.1","unstructured":"Domell\u00f6f, F.P. (2019). The Extraocular Muscles Are Selectively Spared in ALS. In Amyotrophic Lateral Sclerosis-Recent Advances and Therapeutic Challenges. (IntechOpen)."},{"key":"2025102702150437000_2024.12.02.626422v2.2","first-page":"1547","article-title":"Unaffected motor endplate occupancy in eye muscles of ALS G93A mouse model","volume":"4","year":"2012","journal-title":"Frontiers in bioscience (Scholar edition"},{"key":"2025102702150437000_2024.12.02.626422v2.3","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.09-5030"},{"key":"2025102702150437000_2024.12.02.626422v2.4","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034640"},{"key":"2025102702150437000_2024.12.02.626422v2.5","doi-asserted-by":"crossref","unstructured":"Li, A. , Yi, J. , Li, X. , Dong, L. , Ostrow, L.W. , Ma, J. , and Zhou, J . (2024). Distinct transcriptomic profile of satellite cells contributes to preservation of neuromuscular junctions in extraocular muscles of ALS mice. Elife 12, RP92644.","DOI":"10.7554\/eLife.92644.4"},{"key":"2025102702150437000_2024.12.02.626422v2.6","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-9961(03)00041-X"},{"key":"2025102702150437000_2024.12.02.626422v2.7","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.1995.1027"},{"key":"2025102702150437000_2024.12.02.626422v2.8","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-07-02534.2000"},{"key":"2025102702150437000_2024.12.02.626422v2.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2016.10.490"},{"key":"2025102702150437000_2024.12.02.626422v2.10","doi-asserted-by":"crossref","unstructured":"Li, Y. , Zhao, T. , Li, J. , Xia, M. , Li, Y. , Wang, X. , Liu, C. , Zheng, T. , Chen, R. , and Kan, D . (2022). Oxidative Stress and 4-hydroxy-2-nonenal (4-HNE): Implications in the Pathogenesis and Treatment of Aging- related Diseases. Journal of Immunology Research 2022.","DOI":"10.1155\/2022\/2233906"},{"key":"2025102702150437000_2024.12.02.626422v2.11","doi-asserted-by":"publisher","DOI":"10.3109\/10715762.2010.498477"},{"key":"2025102702150437000_2024.12.02.626422v2.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2017.01.036"},{"key":"2025102702150437000_2024.12.02.626422v2.13","doi-asserted-by":"publisher","DOI":"10.1021\/tx800465m"},{"key":"2025102702150437000_2024.12.02.626422v2.14","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1111\/j.1365-2621.1993.tb01278.x","article-title":"Interaction of malondialdehyde with biological molecules\u2014new trends about reactivity and significance","volume":"28","year":"1993","journal-title":"International journal of food science & technology"},{"key":"2025102702150437000_2024.12.02.626422v2.15","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/57.5.779S"},{"key":"2025102702150437000_2024.12.02.626422v2.16","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.mrfmmm.2011.03.006","article-title":"DNA damage induced by endogenous aldehydes: current state of knowledge","volume":"711","year":"2011","journal-title":"Mutation Research\/Fundamental and Molecular Mechanisms of Mutagenesis"},{"key":"2025102702150437000_2024.12.02.626422v2.17","doi-asserted-by":"publisher","DOI":"10.1021\/tx800169a"},{"key":"2025102702150437000_2024.12.02.626422v2.18","doi-asserted-by":"publisher","DOI":"10.1021\/tx800369y"},{"key":"2025102702150437000_2024.12.02.626422v2.19","doi-asserted-by":"publisher","DOI":"10.3109\/15376510903530845"},{"key":"2025102702150437000_2024.12.02.626422v2.20","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(03)00070-4"},{"key":"2025102702150437000_2024.12.02.626422v2.21","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1218(86)90051-0"},{"key":"2025102702150437000_2024.12.02.626422v2.22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/biof.5520200101","article-title":"Resveratrol protects against 4-HNE induced oxidative stress and apoptosis in Swiss 3T3 fibroblasts","volume":"20","year":"2004","journal-title":"Biofactors"},{"key":"2025102702150437000_2024.12.02.626422v2.23","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/j.freeradbiomed.2019.08.027","article-title":"Covalent modification of phosphatidylethanolamine by 4-hydroxy-2-nonenal increases sodium permeability across phospholipid bilayer membranes","volume":"143","year":"2019","journal-title":"Free Radical Biology and Medicine"},{"key":"2025102702150437000_2024.12.02.626422v2.24","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.76.2.293"},{"key":"2025102702150437000_2024.12.02.626422v2.25","first-page":"2132","article-title":"The 4-hydroxynonenal mediated oxidative damage of blood proteins and lipids involves secondary lipid peroxidation reactions","volume":"16","year":"2018","journal-title":"Experimental and Therapeutic Medicine"},{"key":"2025102702150437000_2024.12.02.626422v2.26","doi-asserted-by":"crossref","first-page":"4171","DOI":"10.3390\/nu16234171","article-title":"4-Hydroxynonenal from Mitochondrial and Dietary Sources Causes Lysosomal Cell Death for Lifestyle-Related Diseases","volume":"16","year":"2024","journal-title":"Nutrients"},{"key":"2025102702150437000_2024.12.02.626422v2.27","doi-asserted-by":"publisher","DOI":"10.1021\/tx2005184"},{"key":"2025102702150437000_2024.12.02.626422v2.28","first-page":"42","article-title":"Distribution of messenger RNAs for aldehyde dehydrogenase 1, aldehyde dehydrogenase 2, and aldehyde dehydrogenase 5 in human tissues","volume":"44","year":"1996","journal-title":"Journal of investigative medicine: the official publication of the American Federation for Clinical Research"},{"key":"2025102702150437000_2024.12.02.626422v2.29","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2009.08.017"},{"key":"2025102702150437000_2024.12.02.626422v2.30","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M702076200"},{"key":"2025102702150437000_2024.12.02.626422v2.31","doi-asserted-by":"publisher","DOI":"10.1126\/science.8209258"},{"key":"2025102702150437000_2024.12.02.626422v2.32","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2008.06814.x"},{"key":"2025102702150437000_2024.12.02.626422v2.33","first-page":"197","article-title":"Altered expression of cardiac ankyrin repeat protein and its homologue, ankyrin repeat protein with PEST and proline-rich region, in atrophic muscles in amyotrophic lateral sclerosis","volume":"70","year":"2003","journal-title":"Pathobiology"},{"key":"2025102702150437000_2024.12.02.626422v2.34","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1812"},{"key":"2025102702150437000_2024.12.02.626422v2.35","doi-asserted-by":"crossref","unstructured":"Yi, J. , Li, A. , Li, X. , Park, K. , Zhou, X. , Yi, F. , Xiao, Y. , Yoon, D. , Tan, T. , and Ostrow, L.W. (2021). MG53 preserves neuromuscular junction integrity and alleviates ALS disease progression. Antioxidants 10, 1522.","DOI":"10.3390\/antiox10101522"},{"key":"2025102702150437000_2024.12.02.626422v2.36","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E17-06-0359"},{"key":"2025102702150437000_2024.12.02.626422v2.37","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2016.04.002"},{"key":"2025102702150437000_2024.12.02.626422v2.38","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2018.09.008"},{"key":"2025102702150437000_2024.12.02.626422v2.39","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s13395-017-0123-0","article-title":"Absence of physiological Ca 2+ transients is an initial trigger for mitochondrial dysfunction in skeletal muscle following denervation","volume":"7","year":"2017","journal-title":"Skeletal muscle"},{"key":"2025102702150437000_2024.12.02.626422v2.40","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00767.2006"},{"key":"2025102702150437000_2024.12.02.626422v2.41","doi-asserted-by":"crossref","unstructured":"Davis, J. , Davis, D. , Norman, B. , and Piatigorsky, J . (2008). Gene expression of the mouse corneal crystallin Aldh3a1: activation by Pax6, Oct1, and p300. Investigative ophthalmology & visual science 49, 1814-1826.","DOI":"10.1167\/iovs.07-1057"},{"key":"2025102702150437000_2024.12.02.626422v2.42","doi-asserted-by":"publisher","DOI":"10.1038\/nature12343"},{"key":"2025102702150437000_2024.12.02.626422v2.43","first-page":"119","article-title":"Activated satellite cells are present in uninjured extraocular muscles of mature mice","volume":"100","year":"2002","journal-title":"Transactions of the American Ophthalmological Society"},{"key":"2025102702150437000_2024.12.02.626422v2.44","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1038\/s42003-021-02334-4","article-title":"Nuclear factor E2-related factor 2 (NRF2) deficiency accelerates fast fibre type transition in soleus muscle during space flight","volume":"4","year":"2021","journal-title":"Communications Biology"},{"key":"2025102702150437000_2024.12.02.626422v2.45","first-page":"247","article-title":"Three different stable human breast adenocarcinoma sublines that overexpress ALDH3A1 and certain other enzymes, apparently as a consequence of constitutively upregulated gene transcription mediated by transactivated EpREs (electrophile responsive elements) present in the 5\u2019 upstream regions of these genes","volume":"130","year":"2001","journal-title":"Chemico-Biological Interactions"},{"key":"2025102702150437000_2024.12.02.626422v2.46","doi-asserted-by":"crossref","unstructured":"Baird, L. , and Yamamoto, M . (2020). The Molecular Mechanisms Regulating the KEAP1-NRF2 Pathway. Mol Cell Biol 40.","DOI":"10.1128\/MCB.00099-20"},{"key":"2025102702150437000_2024.12.02.626422v2.47","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(91)90192-6"},{"key":"2025102702150437000_2024.12.02.626422v2.48","doi-asserted-by":"publisher","DOI":"10.1007\/BF03044961"},{"key":"2025102702150437000_2024.12.02.626422v2.49","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(83)90438-6"},{"key":"2025102702150437000_2024.12.02.626422v2.50","doi-asserted-by":"publisher","DOI":"10.3390\/cells11203263"},{"key":"2025102702150437000_2024.12.02.626422v2.51","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M503698200"},{"key":"2025102702150437000_2024.12.02.626422v2.52","doi-asserted-by":"crossref","first-page":"5845","DOI":"10.3390\/ijms24065845","article-title":"Investigating the functional roles of aldehyde dehydrogenase 3A1 in human corneal epithelial cells","volume":"24","year":"2023","journal-title":"International Journal of Molecular Sciences"},{"key":"2025102702150437000_2024.12.02.626422v2.53","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2006.08.009"},{"key":"2025102702150437000_2024.12.02.626422v2.54","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2020.01.183"},{"key":"2025102702150437000_2024.12.02.626422v2.55","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5383.1677"},{"key":"2025102702150437000_2024.12.02.626422v2.56","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.169730"},{"key":"2025102702150437000_2024.12.02.626422v2.57","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00380.2013"},{"key":"2025102702150437000_2024.12.02.626422v2.58","doi-asserted-by":"crossref","first-page":"101966","DOI":"10.1016\/j.redox.2021.101966","article-title":"Skeletal muscle-specific Keap1 disruption modulates fatty acid utilization and enhances exercise capacity in female mice","volume":"43","year":"2021","journal-title":"Redox Biology"},{"key":"2025102702150437000_2024.12.02.626422v2.59","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00347.2019"},{"key":"2025102702150437000_2024.12.02.626422v2.60","doi-asserted-by":"publisher","DOI":"10.1002\/path.4418"},{"key":"2025102702150437000_2024.12.02.626422v2.61","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201500153"},{"key":"2025102702150437000_2024.12.02.626422v2.62","first-page":"5977","article-title":"Nrf2 deficiency promotes the increasing trend of autophagy during aging in skeletal muscle: a potential mechanism for the development of sarcopenia","volume":"12","year":"2020","journal-title":"Aging (Albany NY"},{"key":"2025102702150437000_2024.12.02.626422v2.63","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.redox.2018.04.004","article-title":"Nrf2 deficiency exacerbates age-related contractile dysfunction and loss of skeletal muscle mass","volume":"17","year":"2018","journal-title":"Redox biology"},{"key":"2025102702150437000_2024.12.02.626422v2.64","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2021.07.022"},{"key":"2025102702150437000_2024.12.02.626422v2.65","doi-asserted-by":"publisher","DOI":"10.14740\/jnr589"},{"key":"2025102702150437000_2024.12.02.626422v2.66","first-page":"0","article-title":"Skeletal muscle fiber types in C57BL6J mice","volume":"21","year":"2017","journal-title":"Journal of Morphological Sciences"},{"key":"2025102702150437000_2024.12.02.626422v2.67","doi-asserted-by":"crossref","first-page":"R817","DOI":"10.1152\/ajpregu.00058.2003","article-title":"Fiber-type differences in muscle mitochondrial profiles. American Journal of Physiology-Regulatory","volume":"285","year":"2003","journal-title":"Integrative and Comparative Physiology"},{"key":"2025102702150437000_2024.12.02.626422v2.68","first-page":"3904645","article-title":"Aldehyde dehydrogenases: not just markers, but functional regulators of stem cells","volume":"2019","year":"2019","journal-title":"Stem cells international"},{"key":"2025102702150437000_2024.12.02.626422v2.69","doi-asserted-by":"crossref","first-page":"237","DOI":"10.2174\/157488808786734006","article-title":"Aldehyde dehydrogenase as a marker for stem cells","volume":"3","year":"2008","journal-title":"Current stem cell research & therapy"},{"key":"2025102702150437000_2024.12.02.626422v2.70","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2011.11.033"},{"key":"2025102702150437000_2024.12.02.626422v2.71","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410440518"},{"key":"2025102702150437000_2024.12.02.626422v2.72","doi-asserted-by":"crossref","first-page":"1306","DOI":"10.1021\/acs.chemrestox.4c00052","article-title":"Quantitative Analysis of Glutathione and Carnosine Adducts with 4-Hydroxy-2-nonenal in Muscle in a hSOD1G93A ALS Rat Model","volume":"37","year":"2024","journal-title":"Chemical Research in Toxicology"},{"key":"2025102702150437000_2024.12.02.626422v2.73","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2004.12.021"},{"key":"2025102702150437000_2024.12.02.626422v2.74","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.109.215822"},{"key":"2025102702150437000_2024.12.02.626422v2.75","doi-asserted-by":"crossref","unstructured":"Milkovic, L. , Zarkovic, N. , Marusic, Z. , Zarkovic, K. , and Jaganjac, M. (2023). The 4-Hydroxynonenal\u2013 Protein Adducts and Their Biological Relevance: Are Some Proteins Preferred Targets? Antioxidants 12, 856.","DOI":"10.3390\/antiox12040856"},{"key":"2025102702150437000_2024.12.02.626422v2.76","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2013.138"},{"key":"2025102702150437000_2024.12.02.626422v2.77","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-017-1708-8"},{"key":"2025102702150437000_2024.12.02.626422v2.78","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201695709"},{"key":"2025102702150437000_2024.12.02.626422v2.79","doi-asserted-by":"publisher","DOI":"10.1080\/10520290701488113"},{"key":"2025102702150437000_2024.12.02.626422v2.80","doi-asserted-by":"publisher","DOI":"10.1080\/01478885.2022.2111744"},{"key":"2025102702150437000_2024.12.02.626422v2.81","doi-asserted-by":"publisher","DOI":"10.1093\/NAR\/GKZ114"},{"key":"2025102702150437000_2024.12.02.626422v2.82","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"2025102702150437000_2024.12.02.626422v2.83","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-019-2669-9"},{"key":"2025102702150437000_2024.12.02.626422v2.84","doi-asserted-by":"crossref","first-page":"e411","DOI":"10.1002\/cpz1.411","article-title":"Interactive and Reproducible Workflows for Exploring and Modeling RNA-seq Data with pcaExplorer","volume":"2","year":"2022","journal-title":"Ideal, and GeneTonic. Current protocols"},{"key":"2025102702150437000_2024.12.02.626422v2.85","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2011.0118"},{"key":"2025102702150437000_2024.12.02.626422v2.86","doi-asserted-by":"crossref","first-page":"5432","DOI":"10.1038\/s41467-020-19230-w","article-title":"Identification of a myotropic AAV by massively parallel in vivo evaluation of barcoded capsid variants","volume":"11","year":"2020","journal-title":"Nature communications"},{"key":"2025102702150437000_2024.12.02.626422v2.87","doi-asserted-by":"publisher","DOI":"10.1038\/gt.2008.104"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2024.12.02.626422","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T05:09:16Z","timestamp":1782277756000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2024.12.02.626422"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,4]]},"references-count":87,"URL":"https:\/\/doi.org\/10.1101\/2024.12.02.626422","relation":{"is-preprint-of":[{"id-type":"doi","id":"10.1016\/j.freeradbiomed.2026.03.037","asserted-by":"subject"}]},"subject":[],"published":{"date-parts":[[2024,12,4]]},"subtype":"preprint"}}