{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T18:29:11Z","timestamp":1774549751885,"version":"3.50.1"},"posted":{"date-parts":[[2024,12,3]]},"group-title":"Molecular Biology","reference-count":63,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T00:00:00Z","timestamp":1733184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,12,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  The obligate intracellular bacterial pathogen,\n                  <jats:italic>Chlamydia trachomatis<\/jats:italic>\n                  (Ct), has a distinct DNA topoisomerase I (TopA) with a C-terminal domain (CTD) homologous to eukaryotic SWIB domains. Despite the lack of sequence similarity at the CTDs between\n                  <jats:italic>C. trachomatis<\/jats:italic>\n                  TopA (CtTopA) and\n                  <jats:italic>Escherichia coli<\/jats:italic>\n                  TopA (EcTopA), full-length CtTopA removed negative DNA supercoils\n                  <jats:italic>in vitro<\/jats:italic>\n                  and complemented the growth defect of an\n                  <jats:italic>E. coli topA<\/jats:italic>\n                  mutant. We demonstrated that CtTopA is less processive in DNA relaxation than EcTopA in dose-response and time course studies. An antibody generated against the SWIB domain of CtTopA specifically recognized CtTopA but not EcTopA or\n                  <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                  TopA (MtTopA), consistent with the sequence differences in their CTDs. The endogenous CtTopA protein is expressed at a relatively high level during the middle and late developmental stages of\n                  <jats:italic>C. trachomatis<\/jats:italic>\n                  . Conditional knockdown of\n                  <jats:italic>topA<\/jats:italic>\n                  expression using CRISPRi in\n                  <jats:italic>C. trachomatis<\/jats:italic>\n                  resulted in not only a developmental defect but also in the downregulation of genes linked to nucleotide acquisition from the host cells. Because SWIB-containing proteins are not found in prokaryotes beyond\n                  <jats:italic>Chlamydia<\/jats:italic>\n                  spp., these results imply a significant function for the SWIB-containing CtTopA in facilitating the energy metabolism of\n                  <jats:italic>C. trachomatis<\/jats:italic>\n                  for its unique intracellular growth.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Importance<\/jats:title>\n                  <jats:p>\n                    <jats:italic>C. trachomatis<\/jats:italic>\n                    (Ct) is a medically important bacterial pathogen that is responsible for the most prevalent sexually transmitted bacterial infection. Bioinformatics, genetics, and biochemical analyses have established that the presence of a SWIB domain in CtTopA, a DNA topoisomerase I, is relevant to chlamydial physiology. Further defining the mechanisms of the C-terminal SWIB domain on the catalytic function of CtTopA in an intracellular pathogen is warranted for a more complete understanding of the interactions between\n                    <jats:italic>C. trachomatis<\/jats:italic>\n                    and its host cells.\n                  <\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2024.12.03.626651","type":"posted-content","created":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T22:50:36Z","timestamp":1733266236000},"source":"Crossref","is-referenced-by-count":1,"title":["The SWIB domain-containing DNA topoisomerase I of\n                  <i>Chlamydia trachomatis<\/i>\n                  mediates DNA relaxation"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9869-7317","authenticated-orcid":false,"given":"Li","family":"Shen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caitlynn","family":"Diggs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shomita","family":"Ferdous","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda","family":"Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neol","family":"Wolf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Terrebonne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Lorenzo","family":"Carvajal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7053-5009","authenticated-orcid":false,"given":"Guangming","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3721-6839","authenticated-orcid":false,"given":"Scot P.","family":"Ouellette","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuk-Ching","family":"Tse-Dinh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024120506554170000_2024.12.03.626651v1.1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.202000286"},{"key":"2024120506554170000_2024.12.03.626651v1.2","doi-asserted-by":"publisher","DOI":"10.3390\/cells13060553"},{"key":"2024120506554170000_2024.12.03.626651v1.3","doi-asserted-by":"publisher","DOI":"10.1038\/nrm831"},{"key":"2024120506554170000_2024.12.03.626651v1.4","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9010086"},{"key":"2024120506554170000_2024.12.03.626651v1.5","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.4c00128"},{"key":"2024120506554170000_2024.12.03.626651v1.6","doi-asserted-by":"publisher","DOI":"10.1038\/nri1551"},{"key":"2024120506554170000_2024.12.03.626651v1.7","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiab610"},{"key":"2024120506554170000_2024.12.03.626651v1.8","unstructured":"8. 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