{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T22:46:20Z","timestamp":1768517180293,"version":"3.49.0"},"posted":{"date-parts":[[2024,12,4]]},"group-title":"Microbiology","reference-count":98,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,12,4]],"date-time":"2024-12-04T00:00:00Z","timestamp":1733270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,12,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:sec>\n                  <jats:title>Purpose<\/jats:title>\n                  <jats:p>\n                    Healthy corneas resist colonization by virtually all microbes yet contact lens wear can predispose the cornea to sight-threatening infection with\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    . Here, we explored how lens wear changes corneal epithelium transcriptional responses to\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    and its impact on bacterial gene expression.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>\n                    Male and female C57BL\/6J mice were fitted with a contact lens on one eye for 24 h. After lens removal, corneas were immediately challenged for 4 h with\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    . A separate group of na\u00efve mice were similarly challenged with bacteria. Bacteria-challenged eyes were compared to uninoculated naive controls as was lens wear alone. Total RNA-sequencing determined corneal epithelium and bacterial gene expression.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>\n                    Prior lens wear profoundly altered the corneal response to\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    , including: upregulated pattern-recognition receptors (\n                    <jats:italic>tlr3, nod1<\/jats:italic>\n                    ), downregulated lectin pathway of complement activation (\n                    <jats:italic>masp1<\/jats:italic>\n                    ), amplified upregulation of\n                    <jats:italic>tcf7<\/jats:italic>\n                    ,\n                    <jats:italic>gpr55, ifi205, wfdc2<\/jats:italic>\n                    (immune defense) and further suppression of\n                    <jats:italic>efemp1<\/jats:italic>\n                    (corneal stromal integrity). Without lens wear,\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    upregulated mitochondrial and ubiquinone metabolism genes. Lens wear alone upregulated\n                    <jats:italic>axl, grn, tcf7, gpr55<\/jats:italic>\n                    (immune defense) and downregulated Ca2\n                    <jats:sup>+<\/jats:sup>\n                    -dependent genes\n                    <jats:italic>necab1, snx31 and npr3<\/jats:italic>\n                    .\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    exposure to prior lens wearing vs. na\u00efve corneas upregulated bacterial genes of virulence (\n                    <jats:italic>popD<\/jats:italic>\n                    ), its regulation (\n                    <jats:italic>rsmY<\/jats:italic>\n                    , PA1226) and antimicrobial resistance (\n                    <jats:italic>arnB<\/jats:italic>\n                    ,\n                    <jats:italic>oprR<\/jats:italic>\n                    ).\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>\n                    Prior lens wear impacts corneal epithelium gene expression altering its responses to\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    and how\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    responds to it favoring virulence, survival and adaptation. Impacted genes and associated networks provide avenues for research to better understand infection pathogenesis.\n                  <\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2024.12.03.626720","type":"posted-content","created":{"date-parts":[[2024,12,4]],"date-time":"2024-12-04T22:55:13Z","timestamp":1733352913000},"source":"Crossref","is-referenced-by-count":0,"title":["Contact Lens Wear Alters Transcriptional Responses to\n                  <i>Pseudomonas aeruginosa<\/i>\n                  in Both the Corneal Epithelium and the Bacteria"],"prefix":"10.64898","author":[{"given":"Naren G.","family":"Kumar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melinda R","family":"Grosser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie","family":"Wan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Schator","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugene","family":"Ahn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Jedel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent","family":"Nieto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David J.","family":"Evans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzanne M. 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