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The study included healthy volunteers who underwent MRI scanning with specific VSSL parameters optimized for CSF flow quantification. The VSSL sequence was calibrated against phase-contrast MRI (PC-MRI) to ensure accurate flow velocity measurements. The CSF flow patterns observed in the ventricles were consistent with those obtained using 3D amplified MRI and other advanced MRI techniques, verifying the reliability of the VSSL method. The VSSL method successfully measured CSF flow in the SAS along major arteries, including the middle cerebral artery (MCA), anterior cerebral artery (ACA), and posterior cerebral artery (PCA), with an average flow velocity of 0.339 \u00b1 0.117\n                  <jats:italic>cm<\/jats:italic>\n                  \/\n                  <jats:italic>s<\/jats:italic>\n                  . The diffusion component was well suppressed by flow-compensated gradients, enabling comprehensive mapping of the rapid CSF flow pattern in the SAS system and ventricles. The flow pattern in the SAS system closely resembles the recently discovered perivascular subarachnoid space (PVSAS) system. CSF flow around the MCA, PCA, and ACA arteries in the SAS exhibited a weak orientation dependency. CSF flow in the ventricles was also measured, with an average flow velocity of 0.309 \u00b1 0.116\n                  <jats:italic>cm<\/jats:italic>\n                  \/\n                  <jats:italic>s<\/jats:italic>\n                  , and the highest velocity observed along the superior-inferior direction. This study underscores the potential of VSSL MRI as a non-invasive tool for investigating CSF dynamics in both SAS and ventricles.\n                <\/jats:p>","DOI":"10.1101\/2024.12.09.24318672","type":"posted-content","created":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T18:25:21Z","timestamp":1733855121000},"source":"Crossref","is-referenced-by-count":1,"title":["Cerebrospinal Fluid Flow within Ventricles and Subarachnoid Space Evaluated by Velocity Selective Spin Labeling MRI"],"prefix":"10.64898","author":[{"given":"Yihan","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kexin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"Qin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiadi","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024121220004702000_2024.12.09.24318672v1.1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3003748"},{"key":"2024121220004702000_2024.12.09.24318672v1.2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI67677"},{"key":"2024121220004702000_2024.12.09.24318672v1.3","doi-asserted-by":"crossref","unstructured":"Absinta M , Ha SK , Nair G , Sati P , Luciano NJ , Palisoc M , Louveau A , Zaghloul KA , Pittaluga S , Kipnis J , Reich DS . 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