{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T05:56:22Z","timestamp":1782280582075,"version":"3.54.5"},"posted":{"date-parts":[[2024,12,12]]},"group-title":"Cell Biology","reference-count":30,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T00:00:00Z","timestamp":1733961600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,12,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Airway multiciliated cells (MCs) maintain respiratory health by clearing mucus and trapped particles through the beating of motile cilia. While it is known that ciliary lengths decrease along the proximal-distal (P-D) axis of the tracheobronchial tree, how this is regulated is unclear. Here, we demonstrate that canonical Notch signaling in MCs plays a critical role in stabilizing ciliary length. Inhibition of Notch signaling in MCs results in ciliary shortening in the trachea, lengthening in the distal airway, and to region-specific alterations in gene expression. We probe how environmental challenges impact MC homeostasis using germ-free and\n                  <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                  (\n                  <jats:italic>M. tb<\/jats:italic>\n                  ) infection models. While germ-free conditions do not perturb ciliary lengths,\n                  <jats:italic>M. tb<\/jats:italic>\n                  infection leads to lengthening of distal airway cilia, correlating with a downregulation of Notch signaling. These findings reveal that ciliary length and the P-D gradient in the airways are actively regulated, with Notch signaling serving as a stabilizing mechanism.\n                <\/jats:p>","DOI":"10.1101\/2024.12.12.628112","type":"posted-content","created":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T23:14:44Z","timestamp":1734045284000},"source":"Crossref","is-referenced-by-count":3,"title":["Notch signaling stabilizes lengths of motile cilia in multiciliated cells in the lung"],"prefix":"10.64898","author":[{"given":"Neenu","family":"Joy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aditya","family":"Deshpande","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0555-7727","authenticated-orcid":false,"given":"Sai Manoz","family":"Lingamallu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasam Manjveekar","family":"Prabantu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"CN","family":"Naveenkumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Bharathkumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukanya","family":"Bhat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zabdiel","family":"Alvarado-Martinez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Livraghi-Butrico","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James S.","family":"Hagood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard C.","family":"Boucher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Lafkas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin M.","family":"Byrd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shridhar","family":"Narayanan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.K.","family":"Shandil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arjun","family":"Guha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2024121405452882000_2024.12.12.628112v1.1","doi-asserted-by":"crossref","unstructured":"Ishikawa T . 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Temporal and spatial distribution of ciliogenesis in the tracheobronchial airways of mice. American Journal of Physiology-lung Cellular and Molecular Physiology. 2005.","DOI":"10.1152\/ajplung.00036.2005"},{"key":"2024121405452882000_2024.12.12.628112v1.6","doi-asserted-by":"crossref","unstructured":"Hayashi T , Kawakami M , Sasaki S et al. ATP regulation of ciliary beat frequency in rat tracheal and distal airway epithelium. Exp Physiol. 2005.","DOI":"10.1113\/expphysiol.2004.028746"},{"key":"2024121405452882000_2024.12.12.628112v1.7","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2005-0417OC"},{"key":"2024121405452882000_2024.12.12.628112v1.8","doi-asserted-by":"crossref","unstructured":"Serra CFH , Liu H , Qian J , et al. 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