{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T16:09:17Z","timestamp":1782576557292,"version":"3.54.5"},"posted":{"date-parts":[[2024,12,17]]},"group-title":"Immunology","reference-count":21,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T00:00:00Z","timestamp":1734393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2024,12,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Atherosclerosis, a major contributor to cardiovascular disease, involves lipid accumulation and inflammatory processes in arterial walls, with oxidized low-density lipoprotein (OxLDL) playing a central role. OxLDL is increased during aging and stimulates monocyte transformation into foam cells and induces metabolic reprogramming and pro-inflammatory responses, accelerating atherosclerosis progression and contributing to other age-related diseases. This study investigated the effects of Mdivi-1, a mitochondrial fission inhibitor, and S1QEL, a selective complex I-associated reactive oxygen species (ROS) inhibitor, on OxLDL-induced responses in monocytes. Healthy monocytes isolated from participants were treated with OxLDL, with or without Mdivi-1 or S1QEL, and assessed for metabolic shifts, inflammatory cytokine expression, foam cell formation, and ROS production. OxLDL treatment elevated glycolytic activity (ECAR) and expression of pro-inflammatory cytokines IL1B and CXCL8, promoting foam cell formation and mitochondrial ROS (mtROS) production. Mdivi-1 and S1QEL effectively reduced OxLDL-induced glycolytic reprogramming, inflammatory cytokine levels, and foam cell formation while limiting mtROS. These findings suggest that both Mdivi-1 and S1QEL modulate key monocyte responses to OxLDL, providing insights into potential therapeutic approaches for age-related diseases.<\/jats:p>","DOI":"10.1101\/2024.12.12.628145","type":"posted-content","created":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T16:55:55Z","timestamp":1734713755000},"source":"Crossref","is-referenced-by-count":1,"title":["Modulatory Effects of Mdivi-1 on OxLDL-Induced Metabolic Alterations, Inflammatory Responses, and Foam Cell Formation in Human Monocytes"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7518-8066","authenticated-orcid":false,"given":"Negin","family":"Mosalmanzadeh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1759-1778","authenticated-orcid":false,"given":"Rafael Moura","family":"Maurmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kierstin","family":"Davis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5823-2611","authenticated-orcid":false,"given":"Brenda Landvoigt","family":"Schmitt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5337-8037","authenticated-orcid":false,"given":"Liza","family":"Makowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4059-9092","authenticated-orcid":false,"given":"Brandt D.","family":"Pence","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2024122100283322000_2024.12.12.628145v1.1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2001.tb11378.x"},{"key":"2024122100283322000_2024.12.12.628145v1.2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2001.tb03943.x"},{"key":"2024122100283322000_2024.12.12.628145v1.3","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.16.1.4"},{"key":"2024122100283322000_2024.12.12.628145v1.4","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.114.302721"},{"key":"2024122100283322000_2024.12.12.628145v1.5","doi-asserted-by":"publisher","DOI":"10.1007\/s10522-009-9234-2"},{"key":"2024122100283322000_2024.12.12.628145v1.6","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00003.2017"},{"key":"2024122100283322000_2024.12.12.628145v1.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2016.03.008"},{"key":"2024122100283322000_2024.12.12.628145v1.8","doi-asserted-by":"publisher","DOI":"10.1038\/nri3070"},{"key":"2024122100283322000_2024.12.12.628145v1.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2017.08.006"},{"key":"2024122100283322000_2024.12.12.628145v1.10","doi-asserted-by":"crossref","unstructured":"Faas, M. 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